Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS04500 and HSERO_RS04505 are separated by 147 nucleotides HSERO_RS04505 and HSERO_RS04510 are separated by 30 nucleotides HSERO_RS04510 and HSERO_RS04515 overlap by 4 nucleotides HSERO_RS04515 and HSERO_RS04520 overlap by 47 nucleotides
HSERO_RS04500: HSERO_RS04500 - oxidoreductase, at 988,651 to 989,295
_RS04500
HSERO_RS04505: HSERO_RS04505 - hypothetical protein, at 989,443 to 989,748
_RS04505
HSERO_RS04510: HSERO_RS04510 - tail protein, at 989,779 to 990,246
_RS04510
HSERO_RS04515: HSERO_RS04515 - tail fiber protein, at 990,243 to 990,728
_RS04515
HSERO_RS04520: HSERO_RS04520 - lysozyme, at 990,682 to 991,251
_RS04520
Position (kb)
989
990
991 Strain fitness (log2 ratio)
-1
0
1 at 988.800 kb on + strand, within HSERO_RS04500 at 989.050 kb on - strand, within HSERO_RS04500 at 989.050 kb on - strand, within HSERO_RS04500 at 989.064 kb on - strand, within HSERO_RS04500 at 989.099 kb on + strand, within HSERO_RS04500 at 989.219 kb on + strand, within HSERO_RS04500 at 989.272 kb on - strand at 989.274 kb on + strand at 989.342 kb on + strand at 989.361 kb on + strand at 989.417 kb on + strand at 989.417 kb on + strand at 989.430 kb on - strand at 989.516 kb on - strand, within HSERO_RS04505 at 989.572 kb on + strand, within HSERO_RS04505 at 989.593 kb on - strand, within HSERO_RS04505 at 989.635 kb on - strand, within HSERO_RS04505 at 989.655 kb on + strand, within HSERO_RS04505 at 989.657 kb on - strand, within HSERO_RS04505 at 989.718 kb on - strand at 989.727 kb on + strand at 989.728 kb on - strand at 989.745 kb on - strand at 989.765 kb on - strand at 989.857 kb on - strand, within HSERO_RS04510 at 989.961 kb on - strand, within HSERO_RS04510 at 990.287 kb on - strand at 990.405 kb on - strand, within HSERO_RS04515 at 990.438 kb on + strand, within HSERO_RS04515 at 990.438 kb on + strand, within HSERO_RS04515 at 990.446 kb on - strand, within HSERO_RS04515 at 990.577 kb on - strand, within HSERO_RS04515 at 990.659 kb on + strand, within HSERO_RS04515 at 990.666 kb on + strand, within HSERO_RS04515 at 991.069 kb on + strand, within HSERO_RS04520 at 991.156 kb on + strand, within HSERO_RS04520 at 991.156 kb on + strand, within HSERO_RS04520
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 988,800 + HSERO_RS04500 0.23 +0.5 989,050 - HSERO_RS04500 0.62 -0.2 989,050 - HSERO_RS04500 0.62 -0.4 989,064 - HSERO_RS04500 0.64 +0.3 989,099 + HSERO_RS04500 0.69 -0.3 989,219 + HSERO_RS04500 0.88 +0.6 989,272 - +0.6 989,274 + -0.5 989,342 + -0.9 989,361 + -0.5 989,417 + +0.1 989,417 + -0.6 989,430 - -0.5 989,516 - HSERO_RS04505 0.24 -0.7 989,572 + HSERO_RS04505 0.42 -0.8 989,593 - HSERO_RS04505 0.49 +0.3 989,635 - HSERO_RS04505 0.63 +1.2 989,655 + HSERO_RS04505 0.69 -1.0 989,657 - HSERO_RS04505 0.70 +0.4 989,718 - +0.8 989,727 + +0.5 989,728 - -0.1 989,745 - +0.1 989,765 - -0.8 989,857 - HSERO_RS04510 0.17 +0.4 989,961 - HSERO_RS04510 0.39 +0.4 990,287 - -0.5 990,405 - HSERO_RS04515 0.33 -0.4 990,438 + HSERO_RS04515 0.40 +0.3 990,438 + HSERO_RS04515 0.40 +0.3 990,446 - HSERO_RS04515 0.42 -0.1 990,577 - HSERO_RS04515 0.69 -0.2 990,659 + HSERO_RS04515 0.86 -0.3 990,666 + HSERO_RS04515 0.87 -0.2 991,069 + HSERO_RS04520 0.68 -0.2 991,156 + HSERO_RS04520 0.83 +0.5 991,156 + HSERO_RS04520 0.83 -0.1
Or see this region's nucleotide sequence