Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS02420 and HSERO_RS02425 are separated by 24 nucleotides HSERO_RS02425 and HSERO_RS02430 are separated by 182 nucleotides HSERO_RS02430 and HSERO_RS02435 are separated by 19 nucleotides HSERO_RS02435 and HSERO_RS02440 are separated by 151 nucleotides
HSERO_RS02420: HSERO_RS02420 - TetR family transcriptional regulator, at 531,912 to 532,547
_RS02420
HSERO_RS02425: HSERO_RS02425 - transcriptional activator, at 532,572 to 533,069
_RS02425
HSERO_RS02430: HSERO_RS02430 - sulfurtransferase, at 533,252 to 533,575
_RS02430
HSERO_RS02435: HSERO_RS02435 - protein-L-isoaspartate O-methyltransferase, at 533,595 to 534,260
_RS02435
HSERO_RS02440: HSERO_RS02440 - TetR family transcriptional regulator, at 534,412 to 535,041
_RS02440
Position (kb) 533
534 Strain fitness (log2 ratio) -1
0
1
2 at 532.470 kb on - strand, within HSERO_RS02420 at 532.568 kb on + strand at 532.643 kb on + strand, within HSERO_RS02425 at 532.732 kb on - strand, within HSERO_RS02425 at 532.872 kb on + strand, within HSERO_RS02425 at 532.881 kb on + strand, within HSERO_RS02425 at 532.889 kb on - strand at 533.023 kb on + strand at 533.031 kb on - strand at 533.072 kb on - strand at 533.309 kb on - strand, within HSERO_RS02430 at 533.392 kb on + strand, within HSERO_RS02430 at 533.413 kb on + strand, within HSERO_RS02430 at 533.564 kb on + strand at 534.329 kb on - strand at 534.366 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 532,470 - HSERO_RS02420 0.88 -0.7 532,568 + -0.1 532,643 + HSERO_RS02425 0.14 -0.4 532,732 - HSERO_RS02425 0.32 -0.4 532,872 + HSERO_RS02425 0.60 +0.4 532,881 + HSERO_RS02425 0.62 -0.1 532,889 - +0.1 533,023 + -0.2 533,031 - -1.4 533,072 - +0.3 533,309 - HSERO_RS02430 0.18 -0.0 533,392 + HSERO_RS02430 0.43 +0.5 533,413 + HSERO_RS02430 0.50 +2.1 533,564 + +0.5 534,329 - -0.3 534,366 - +0.5
Or see this region's nucleotide sequence