Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS02340 and HSERO_RS02345 are separated by 114 nucleotides HSERO_RS02345 and HSERO_RS02350 overlap by 71 nucleotides HSERO_RS02350 and HSERO_RS02355 are separated by 226 nucleotides
HSERO_RS02340: HSERO_RS02340 - dihydroorotase, at 510,166 to 511,452
_RS02340
HSERO_RS02345: HSERO_RS02345 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 511,567 to 512,367
_RS02345
HSERO_RS02350: HSERO_RS02350 - diadenosine tetraphosphatase, at 512,297 to 513,121
_RS02350
HSERO_RS02355: HSERO_RS02355 - phosphoglucomutase, at 513,348 to 514,724
_RS02355
Position (kb) 511
512
513 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 510.567 kb on + strand, within HSERO_RS02340 at 510.576 kb on - strand, within HSERO_RS02340 at 510.576 kb on - strand, within HSERO_RS02340 at 510.645 kb on - strand, within HSERO_RS02340 at 510.726 kb on - strand, within HSERO_RS02340 at 510.800 kb on + strand, within HSERO_RS02340 at 510.808 kb on - strand, within HSERO_RS02340 at 510.900 kb on + strand, within HSERO_RS02340 at 510.905 kb on + strand, within HSERO_RS02340 at 510.908 kb on + strand, within HSERO_RS02340 at 510.955 kb on - strand, within HSERO_RS02340 at 511.076 kb on + strand, within HSERO_RS02340 at 511.081 kb on + strand, within HSERO_RS02340 at 511.084 kb on - strand, within HSERO_RS02340 at 511.129 kb on + strand, within HSERO_RS02340 at 511.216 kb on - strand, within HSERO_RS02340 at 511.218 kb on + strand, within HSERO_RS02340 at 511.229 kb on + strand, within HSERO_RS02340 at 511.272 kb on - strand, within HSERO_RS02340 at 511.347 kb on - strand at 511.605 kb on + strand at 511.646 kb on + strand at 511.740 kb on - strand, within HSERO_RS02345 at 511.925 kb on - strand, within HSERO_RS02345 at 511.996 kb on + strand, within HSERO_RS02345 at 512.062 kb on + strand, within HSERO_RS02345 at 512.070 kb on - strand, within HSERO_RS02345 at 512.070 kb on - strand, within HSERO_RS02345 at 512.075 kb on - strand, within HSERO_RS02345 at 512.077 kb on - strand, within HSERO_RS02345 at 512.173 kb on + strand, within HSERO_RS02345 at 512.208 kb on + strand, within HSERO_RS02345 at 512.216 kb on - strand, within HSERO_RS02345 at 512.361 kb on + strand at 512.369 kb on - strand at 512.445 kb on + strand, within HSERO_RS02350 at 512.485 kb on + strand, within HSERO_RS02350 at 512.506 kb on - strand, within HSERO_RS02350 at 512.585 kb on + strand, within HSERO_RS02350 at 512.842 kb on - strand, within HSERO_RS02350 at 512.914 kb on + strand, within HSERO_RS02350 at 512.914 kb on + strand, within HSERO_RS02350 at 512.922 kb on - strand, within HSERO_RS02350 at 513.070 kb on + strand at 513.189 kb on + strand at 513.364 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 510,567 + HSERO_RS02340 0.31 -4.3 510,576 - HSERO_RS02340 0.32 -0.5 510,576 - HSERO_RS02340 0.32 -5.8 510,645 - HSERO_RS02340 0.37 -4.8 510,726 - HSERO_RS02340 0.44 -3.4 510,800 + HSERO_RS02340 0.49 -3.6 510,808 - HSERO_RS02340 0.50 -5.8 510,900 + HSERO_RS02340 0.57 -0.5 510,905 + HSERO_RS02340 0.57 -5.6 510,908 + HSERO_RS02340 0.58 -4.9 510,955 - HSERO_RS02340 0.61 -3.5 511,076 + HSERO_RS02340 0.71 -4.7 511,081 + HSERO_RS02340 0.71 -4.5 511,084 - HSERO_RS02340 0.71 -5.9 511,129 + HSERO_RS02340 0.75 -1.9 511,216 - HSERO_RS02340 0.82 -2.2 511,218 + HSERO_RS02340 0.82 -5.8 511,229 + HSERO_RS02340 0.83 -3.5 511,272 - HSERO_RS02340 0.86 -2.1 511,347 - -5.2 511,605 + +0.3 511,646 + -0.4 511,740 - HSERO_RS02345 0.22 +1.8 511,925 - HSERO_RS02345 0.45 +0.6 511,996 + HSERO_RS02345 0.54 +0.2 512,062 + HSERO_RS02345 0.62 +0.0 512,070 - HSERO_RS02345 0.63 +0.6 512,070 - HSERO_RS02345 0.63 +0.0 512,075 - HSERO_RS02345 0.63 +1.7 512,077 - HSERO_RS02345 0.64 -2.5 512,173 + HSERO_RS02345 0.76 -0.9 512,208 + HSERO_RS02345 0.80 +0.6 512,216 - HSERO_RS02345 0.81 +0.2 512,361 + +0.1 512,369 - -2.0 512,445 + HSERO_RS02350 0.18 +0.0 512,485 + HSERO_RS02350 0.23 -0.5 512,506 - HSERO_RS02350 0.25 -0.4 512,585 + HSERO_RS02350 0.35 -0.2 512,842 - HSERO_RS02350 0.66 -0.8 512,914 + HSERO_RS02350 0.75 -0.7 512,914 + HSERO_RS02350 0.75 -0.5 512,922 - HSERO_RS02350 0.76 +0.3 513,070 + -0.2 513,189 + -0.8 513,364 - +0.1
Or see this region's nucleotide sequence