Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS02095 and HSERO_RS02100 are separated by 179 nucleotides HSERO_RS02100 and HSERO_RS02105 are separated by 19 nucleotides HSERO_RS02105 and HSERO_RS02110 are separated by 11 nucleotides
HSERO_RS02095: HSERO_RS02095 - Holliday junction DNA helicase RuvB, at 456,149 to 457,204
_RS02095
HSERO_RS02100: HSERO_RS02100 - 4-oxalocrotonate decarboxylase, at 457,384 to 458,226
_RS02100
HSERO_RS02105: HSERO_RS02105 - hypothetical protein, at 458,246 to 458,701
_RS02105
HSERO_RS02110: HSERO_RS02110 - hypothetical protein, at 458,713 to 459,177
_RS02110
Position (kb) 457
458
459 Strain fitness (log2 ratio) -1
0
1 at 456.552 kb on - strand, within HSERO_RS02095 at 456.723 kb on - strand, within HSERO_RS02095 at 457.251 kb on - strand at 457.271 kb on - strand at 457.477 kb on - strand, within HSERO_RS02100 at 457.917 kb on + strand, within HSERO_RS02100 at 458.235 kb on - strand at 458.540 kb on + strand, within HSERO_RS02105 at 458.540 kb on + strand, within HSERO_RS02105 at 458.541 kb on - strand, within HSERO_RS02105 at 458.588 kb on - strand, within HSERO_RS02105 at 458.698 kb on - strand at 458.739 kb on - strand at 458.873 kb on + strand, within HSERO_RS02110 at 459.035 kb on + strand, within HSERO_RS02110 at 459.180 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 456,552 - HSERO_RS02095 0.38 +0.1 456,723 - HSERO_RS02095 0.54 -0.9 457,251 - -0.4 457,271 - -0.0 457,477 - HSERO_RS02100 0.11 +0.3 457,917 + HSERO_RS02100 0.63 +0.9 458,235 - -0.6 458,540 + HSERO_RS02105 0.64 -0.1 458,540 + HSERO_RS02105 0.64 +1.1 458,541 - HSERO_RS02105 0.65 +0.7 458,588 - HSERO_RS02105 0.75 -1.4 458,698 - -0.6 458,739 - +0.5 458,873 + HSERO_RS02110 0.34 -0.3 459,035 + HSERO_RS02110 0.69 -1.3 459,180 - +0.2
Or see this region's nucleotide sequence