Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS01495 and HSERO_RS01500 are separated by 116 nucleotides HSERO_RS01500 and HSERO_RS01505 overlap by 4 nucleotides HSERO_RS01505 and HSERO_RS01510 are separated by 55 nucleotides
HSERO_RS01495: HSERO_RS01495 - Fe-S osidoreductase, at 323,802 to 325,328
_RS01495
HSERO_RS01500: HSERO_RS01500 - transketolase, at 325,445 to 326,263
_RS01500
HSERO_RS01505: HSERO_RS01505 - transketolase, at 326,260 to 327,225
_RS01505
HSERO_RS01510: HSERO_RS01510 - membrane protein, at 327,281 to 328,219
_RS01510
Position (kb) 326
327
328 Strain fitness (log2 ratio) -1
0
1 at 325.348 kb on - strand at 325.388 kb on + strand at 325.803 kb on + strand, within HSERO_RS01500 at 325.811 kb on - strand, within HSERO_RS01500 at 325.816 kb on - strand, within HSERO_RS01500 at 325.877 kb on - strand, within HSERO_RS01500 at 326.145 kb on - strand, within HSERO_RS01500 at 326.145 kb on - strand, within HSERO_RS01500 at 326.225 kb on - strand at 326.330 kb on + strand at 326.476 kb on + strand, within HSERO_RS01505 at 326.814 kb on + strand, within HSERO_RS01505 at 326.899 kb on - strand, within HSERO_RS01505 at 326.996 kb on - strand, within HSERO_RS01505 at 327.181 kb on + strand at 327.271 kb on + strand at 327.332 kb on - strand at 327.714 kb on + strand, within HSERO_RS01510 at 327.724 kb on + strand, within HSERO_RS01510 at 327.840 kb on + strand, within HSERO_RS01510 at 327.856 kb on - strand, within HSERO_RS01510 at 327.916 kb on + strand, within HSERO_RS01510 at 327.946 kb on + strand, within HSERO_RS01510 at 328.145 kb on + strand at 328.145 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 325,348 - +0.3 325,388 + -1.4 325,803 + HSERO_RS01500 0.44 +0.2 325,811 - HSERO_RS01500 0.45 -0.3 325,816 - HSERO_RS01500 0.45 +0.3 325,877 - HSERO_RS01500 0.53 -0.3 326,145 - HSERO_RS01500 0.85 -0.7 326,145 - HSERO_RS01500 0.85 -0.2 326,225 - -0.9 326,330 + -1.4 326,476 + HSERO_RS01505 0.22 +0.7 326,814 + HSERO_RS01505 0.57 -0.0 326,899 - HSERO_RS01505 0.66 +1.5 326,996 - HSERO_RS01505 0.76 +0.3 327,181 + -0.9 327,271 + +0.6 327,332 - -0.4 327,714 + HSERO_RS01510 0.46 +0.0 327,724 + HSERO_RS01510 0.47 +0.3 327,840 + HSERO_RS01510 0.60 -0.4 327,856 - HSERO_RS01510 0.61 +0.9 327,916 + HSERO_RS01510 0.68 -0.6 327,946 + HSERO_RS01510 0.71 -0.8 328,145 + -0.2 328,145 + +0.5
Or see this region's nucleotide sequence