Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS01040 and HSERO_RS01045 overlap by 4 nucleotides HSERO_RS01045 and HSERO_RS01050 are separated by 68 nucleotides HSERO_RS01050 and HSERO_RS01055 are separated by 5 nucleotides
HSERO_RS01040: HSERO_RS01040 - tail fiber protein, at 221,503 to 222,669
_RS01040
HSERO_RS01045: HSERO_RS01045 - bacteriophage baseplate assembly protein W, at 222,666 to 223,091
_RS01045
HSERO_RS01050: HSERO_RS01050 - baseplate protein, at 223,160 to 223,744
_RS01050
HSERO_RS01055: HSERO_RS01055 - bacteriophage regulatory protein, at 223,750 to 224,766
_RS01055
Position (kb)
222
223
224 Strain fitness (log2 ratio)
-1
0
1 at 221.670 kb on - strand, within HSERO_RS01040 at 221.751 kb on - strand, within HSERO_RS01040 at 221.752 kb on - strand, within HSERO_RS01040 at 221.769 kb on + strand, within HSERO_RS01040 at 221.777 kb on - strand, within HSERO_RS01040 at 221.777 kb on - strand, within HSERO_RS01040 at 221.858 kb on + strand, within HSERO_RS01040 at 221.858 kb on + strand, within HSERO_RS01040 at 221.858 kb on + strand, within HSERO_RS01040 at 221.866 kb on - strand, within HSERO_RS01040 at 221.881 kb on + strand, within HSERO_RS01040 at 222.020 kb on - strand, within HSERO_RS01040 at 222.047 kb on + strand, within HSERO_RS01040 at 222.057 kb on + strand, within HSERO_RS01040 at 222.082 kb on + strand, within HSERO_RS01040 at 222.179 kb on + strand, within HSERO_RS01040 at 222.192 kb on - strand, within HSERO_RS01040 at 222.216 kb on - strand, within HSERO_RS01040 at 222.307 kb on + strand, within HSERO_RS01040 at 222.335 kb on - strand, within HSERO_RS01040 at 222.459 kb on - strand, within HSERO_RS01040 at 222.494 kb on - strand, within HSERO_RS01040 at 222.524 kb on + strand, within HSERO_RS01040 at 222.775 kb on + strand, within HSERO_RS01045 at 222.778 kb on - strand, within HSERO_RS01045 at 222.803 kb on + strand, within HSERO_RS01045 at 222.917 kb on - strand, within HSERO_RS01045 at 222.937 kb on - strand, within HSERO_RS01045 at 222.938 kb on + strand, within HSERO_RS01045 at 223.165 kb on - strand at 223.206 kb on + strand at 223.221 kb on - strand, within HSERO_RS01050 at 223.410 kb on + strand, within HSERO_RS01050 at 223.418 kb on - strand, within HSERO_RS01050 at 223.987 kb on - strand, within HSERO_RS01055 at 223.993 kb on - strand, within HSERO_RS01055 at 224.024 kb on - strand, within HSERO_RS01055
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 221,670 - HSERO_RS01040 0.14 +1.6 221,751 - HSERO_RS01040 0.21 -0.4 221,752 - HSERO_RS01040 0.21 -0.9 221,769 + HSERO_RS01040 0.23 +0.5 221,777 - HSERO_RS01040 0.23 -0.1 221,777 - HSERO_RS01040 0.23 -0.0 221,858 + HSERO_RS01040 0.30 +0.8 221,858 + HSERO_RS01040 0.30 -0.5 221,858 + HSERO_RS01040 0.30 -1.0 221,866 - HSERO_RS01040 0.31 -0.1 221,881 + HSERO_RS01040 0.32 -0.3 222,020 - HSERO_RS01040 0.44 +0.6 222,047 + HSERO_RS01040 0.47 +1.5 222,057 + HSERO_RS01040 0.47 +0.2 222,082 + HSERO_RS01040 0.50 +0.4 222,179 + HSERO_RS01040 0.58 -0.4 222,192 - HSERO_RS01040 0.59 +1.0 222,216 - HSERO_RS01040 0.61 -0.1 222,307 + HSERO_RS01040 0.69 -0.5 222,335 - HSERO_RS01040 0.71 +0.4 222,459 - HSERO_RS01040 0.82 -1.3 222,494 - HSERO_RS01040 0.85 -0.5 222,524 + HSERO_RS01040 0.87 +0.1 222,775 + HSERO_RS01045 0.26 +0.9 222,778 - HSERO_RS01045 0.26 +0.1 222,803 + HSERO_RS01045 0.32 +0.1 222,917 - HSERO_RS01045 0.59 +0.8 222,937 - HSERO_RS01045 0.64 -0.2 222,938 + HSERO_RS01045 0.64 +0.7 223,165 - -0.6 223,206 + -0.4 223,221 - HSERO_RS01050 0.10 -0.9 223,410 + HSERO_RS01050 0.43 +0.6 223,418 - HSERO_RS01050 0.44 -0.0 223,987 - HSERO_RS01055 0.23 -0.1 223,993 - HSERO_RS01055 0.24 -1.7 224,024 - HSERO_RS01055 0.27 +0.7
Or see this region's nucleotide sequence