Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS00415 and HSERO_RS00420 are separated by 12 nucleotides HSERO_RS00420 and HSERO_RS00425 are separated by 15 nucleotides HSERO_RS00425 and HSERO_RS00430 are separated by 347 nucleotides
HSERO_RS00415: HSERO_RS00415 - hypothetical protein, at 98,251 to 99,039
_RS00415
HSERO_RS00420: HSERO_RS00420 - nitrogen regulatory protein P-II 1, at 99,052 to 99,390
_RS00420
HSERO_RS00425: HSERO_RS00425 - ammonia channel protein, at 99,406 to 100,938
_RS00425
HSERO_RS00430: HSERO_RS00430 - glutamate--cysteine ligase, at 101,286 to 102,587
_RS00430
Position (kb) 99
100
101 Strain fitness (log2 ratio) -2
-1
0
1
2 at 98.444 kb on - strand, within HSERO_RS00415 at 98.500 kb on + strand, within HSERO_RS00415 at 98.607 kb on + strand, within HSERO_RS00415 at 98.620 kb on - strand, within HSERO_RS00415 at 98.630 kb on - strand, within HSERO_RS00415 at 98.630 kb on - strand, within HSERO_RS00415 at 98.809 kb on - strand, within HSERO_RS00415 at 98.835 kb on + strand, within HSERO_RS00415 at 98.859 kb on + strand, within HSERO_RS00415 at 98.912 kb on + strand, within HSERO_RS00415 at 98.920 kb on - strand, within HSERO_RS00415 at 98.926 kb on - strand at 98.928 kb on + strand, within HSERO_RS00415 at 99.055 kb on + strand at 99.058 kb on - strand at 99.058 kb on - strand at 99.393 kb on - strand at 99.711 kb on + strand, within HSERO_RS00425 at 99.711 kb on + strand, within HSERO_RS00425 at 99.719 kb on - strand, within HSERO_RS00425 at 99.719 kb on - strand, within HSERO_RS00425 at 99.729 kb on - strand, within HSERO_RS00425 at 99.884 kb on - strand, within HSERO_RS00425 at 99.979 kb on - strand, within HSERO_RS00425 at 100.173 kb on + strand, within HSERO_RS00425 at 100.208 kb on - strand, within HSERO_RS00425 at 100.237 kb on - strand, within HSERO_RS00425 at 100.378 kb on + strand, within HSERO_RS00425 at 100.433 kb on - strand, within HSERO_RS00425 at 100.616 kb on + strand, within HSERO_RS00425 at 100.675 kb on + strand, within HSERO_RS00425 at 100.675 kb on + strand, within HSERO_RS00425 at 100.773 kb on + strand, within HSERO_RS00425 at 100.793 kb on - strand at 100.795 kb on + strand at 100.795 kb on + strand at 100.905 kb on - strand at 100.940 kb on - strand at 100.970 kb on - strand at 101.072 kb on + strand at 101.739 kb on - strand, within HSERO_RS00430 at 101.773 kb on + strand, within HSERO_RS00430
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 98,444 - HSERO_RS00415 0.24 -0.1 98,500 + HSERO_RS00415 0.32 +0.4 98,607 + HSERO_RS00415 0.45 +0.6 98,620 - HSERO_RS00415 0.47 +0.1 98,630 - HSERO_RS00415 0.48 -0.1 98,630 - HSERO_RS00415 0.48 -0.9 98,809 - HSERO_RS00415 0.71 -0.2 98,835 + HSERO_RS00415 0.74 -0.3 98,859 + HSERO_RS00415 0.77 +0.1 98,912 + HSERO_RS00415 0.84 +0.6 98,920 - HSERO_RS00415 0.85 +0.4 98,926 - +1.8 98,928 + HSERO_RS00415 0.86 -0.1 99,055 + +1.0 99,058 - -0.8 99,058 - +0.1 99,393 - -0.1 99,711 + HSERO_RS00425 0.20 +0.0 99,711 + HSERO_RS00425 0.20 -0.1 99,719 - HSERO_RS00425 0.20 -0.4 99,719 - HSERO_RS00425 0.20 -0.9 99,729 - HSERO_RS00425 0.21 +0.5 99,884 - HSERO_RS00425 0.31 -0.9 99,979 - HSERO_RS00425 0.37 -0.3 100,173 + HSERO_RS00425 0.50 +0.6 100,208 - HSERO_RS00425 0.52 +0.9 100,237 - HSERO_RS00425 0.54 +0.3 100,378 + HSERO_RS00425 0.63 +0.6 100,433 - HSERO_RS00425 0.67 +0.5 100,616 + HSERO_RS00425 0.79 +0.3 100,675 + HSERO_RS00425 0.83 +0.3 100,675 + HSERO_RS00425 0.83 -0.5 100,773 + HSERO_RS00425 0.89 -0.1 100,793 - -0.2 100,795 + -1.5 100,795 + -0.4 100,905 - -2.3 100,940 - +0.1 100,970 - +0.1 101,072 + -1.3 101,739 - HSERO_RS00430 0.35 -1.3 101,773 + HSERO_RS00430 0.37 +0.3
Or see this region's nucleotide sequence