Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS00080 and HSERO_RS00085 are separated by 47 nucleotides HSERO_RS00085 and HSERO_RS00090 are separated by 263 nucleotides HSERO_RS00090 and HSERO_RS00095 are separated by 19 nucleotides
HSERO_RS00080: HSERO_RS00080 - histidine kinase, at 24,046 to 25,449
_RS00080
HSERO_RS00085: HSERO_RS00085 - transcriptional regulator, at 25,497 to 25,766
_RS00085
HSERO_RS00090: HSERO_RS00090 - hypothetical protein, at 26,030 to 27,307
_RS00090
HSERO_RS00095: HSERO_RS00095 - AMP-dependent synthetase, at 27,327 to 28,946
_RS00095
Position (kb) 26
27
28 Strain fitness (log2 ratio) -2
-1
0
1
2 at 25.060 kb on + strand, within HSERO_RS00080 at 25.068 kb on - strand, within HSERO_RS00080 at 25.114 kb on + strand, within HSERO_RS00080 at 25.341 kb on - strand at 25.441 kb on + strand at 25.444 kb on + strand at 25.446 kb on + strand at 25.447 kb on - strand at 25.519 kb on + strand at 25.519 kb on + strand at 25.519 kb on + strand at 25.519 kb on + strand at 25.519 kb on + strand at 25.527 kb on - strand, within HSERO_RS00085 at 25.527 kb on - strand, within HSERO_RS00085 at 25.527 kb on - strand, within HSERO_RS00085 at 25.576 kb on + strand, within HSERO_RS00085 at 25.588 kb on + strand, within HSERO_RS00085 at 25.588 kb on + strand, within HSERO_RS00085 at 25.640 kb on + strand, within HSERO_RS00085 at 25.647 kb on - strand, within HSERO_RS00085 at 25.691 kb on + strand, within HSERO_RS00085 at 25.698 kb on + strand, within HSERO_RS00085 at 25.698 kb on - strand, within HSERO_RS00085 at 25.699 kb on - strand, within HSERO_RS00085 at 25.736 kb on - strand, within HSERO_RS00085 at 25.767 kb on - strand at 25.784 kb on - strand at 25.846 kb on - strand at 25.899 kb on + strand at 25.902 kb on - strand at 25.955 kb on - strand at 25.980 kb on + strand at 25.991 kb on - strand at 26.166 kb on - strand, within HSERO_RS00090 at 26.176 kb on - strand, within HSERO_RS00090 at 26.282 kb on + strand, within HSERO_RS00090 at 26.283 kb on + strand, within HSERO_RS00090 at 26.287 kb on + strand, within HSERO_RS00090 at 26.290 kb on - strand, within HSERO_RS00090 at 26.290 kb on - strand, within HSERO_RS00090 at 26.291 kb on - strand, within HSERO_RS00090 at 26.479 kb on - strand, within HSERO_RS00090 at 26.557 kb on + strand, within HSERO_RS00090 at 26.623 kb on - strand, within HSERO_RS00090 at 26.850 kb on - strand, within HSERO_RS00090 at 26.912 kb on + strand, within HSERO_RS00090 at 27.007 kb on + strand, within HSERO_RS00090 at 27.500 kb on + strand, within HSERO_RS00095 at 27.500 kb on - strand, within HSERO_RS00095 at 27.824 kb on - strand, within HSERO_RS00095 at 27.824 kb on - strand, within HSERO_RS00095 at 27.845 kb on + strand, within HSERO_RS00095 at 27.845 kb on - strand, within HSERO_RS00095 at 27.860 kb on - strand, within HSERO_RS00095 at 27.955 kb on + strand, within HSERO_RS00095 at 28.183 kb on + strand, within HSERO_RS00095 at 28.194 kb on - strand, within HSERO_RS00095 at 28.249 kb on + strand, within HSERO_RS00095 at 28.292 kb on + strand, within HSERO_RS00095
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 25,060 + HSERO_RS00080 0.72 +0.1 25,068 - HSERO_RS00080 0.73 -0.4 25,114 + HSERO_RS00080 0.76 -1.2 25,341 - +0.4 25,441 + +0.3 25,444 + -0.8 25,446 + -1.3 25,447 - -0.9 25,519 + -0.5 25,519 + +0.9 25,519 + -0.5 25,519 + -0.1 25,519 + -0.1 25,527 - HSERO_RS00085 0.11 +0.2 25,527 - HSERO_RS00085 0.11 +1.0 25,527 - HSERO_RS00085 0.11 +0.7 25,576 + HSERO_RS00085 0.29 -0.2 25,588 + HSERO_RS00085 0.34 -0.2 25,588 + HSERO_RS00085 0.34 -0.7 25,640 + HSERO_RS00085 0.53 -0.3 25,647 - HSERO_RS00085 0.56 +0.4 25,691 + HSERO_RS00085 0.72 -0.2 25,698 + HSERO_RS00085 0.74 +0.0 25,698 - HSERO_RS00085 0.74 +0.4 25,699 - HSERO_RS00085 0.75 -0.7 25,736 - HSERO_RS00085 0.89 +0.7 25,767 - +0.9 25,784 - -1.1 25,846 - +0.9 25,899 + -0.4 25,902 - -2.0 25,955 - -0.2 25,980 + -0.2 25,991 - -0.2 26,166 - HSERO_RS00090 0.11 +0.0 26,176 - HSERO_RS00090 0.11 +0.8 26,282 + HSERO_RS00090 0.20 -0.3 26,283 + HSERO_RS00090 0.20 -0.2 26,287 + HSERO_RS00090 0.20 +0.3 26,290 - HSERO_RS00090 0.20 +1.3 26,290 - HSERO_RS00090 0.20 -0.3 26,291 - HSERO_RS00090 0.20 +1.3 26,479 - HSERO_RS00090 0.35 +0.1 26,557 + HSERO_RS00090 0.41 +0.3 26,623 - HSERO_RS00090 0.46 -0.2 26,850 - HSERO_RS00090 0.64 +0.4 26,912 + HSERO_RS00090 0.69 +1.0 27,007 + HSERO_RS00090 0.76 +1.7 27,500 + HSERO_RS00095 0.11 +0.2 27,500 - HSERO_RS00095 0.11 -1.5 27,824 - HSERO_RS00095 0.31 +0.8 27,824 - HSERO_RS00095 0.31 -0.1 27,845 + HSERO_RS00095 0.32 +0.4 27,845 - HSERO_RS00095 0.32 +2.0 27,860 - HSERO_RS00095 0.33 +0.3 27,955 + HSERO_RS00095 0.39 -0.2 28,183 + HSERO_RS00095 0.53 +0.0 28,194 - HSERO_RS00095 0.54 -0.1 28,249 + HSERO_RS00095 0.57 +0.2 28,292 + HSERO_RS00095 0.60 -0.1
Or see this region's nucleotide sequence