Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS00055 and HSERO_RS00060 are separated by 362 nucleotides HSERO_RS00060 and HSERO_RS00065 are separated by 37 nucleotides
HSERO_RS00055: HSERO_RS00055 - amidase, at 17,667 to 19,115
_RS00055
HSERO_RS00060: HSERO_RS00060 - hypothetical protein, at 19,478 to 20,413
_RS00060
HSERO_RS00065: HSERO_RS00065 - hypothetical protein, at 20,451 to 22,787
_RS00065
Position (kb) 19
20
21 Strain fitness (log2 ratio) -2
-1
0
1
2 at 18.513 kb on - strand, within HSERO_RS00055 at 18.558 kb on + strand, within HSERO_RS00055 at 18.593 kb on + strand, within HSERO_RS00055 at 18.683 kb on - strand, within HSERO_RS00055 at 18.685 kb on + strand, within HSERO_RS00055 at 18.714 kb on + strand, within HSERO_RS00055 at 18.714 kb on + strand, within HSERO_RS00055 at 18.727 kb on - strand, within HSERO_RS00055 at 18.823 kb on + strand, within HSERO_RS00055 at 18.889 kb on + strand, within HSERO_RS00055 at 18.900 kb on - strand, within HSERO_RS00055 at 18.909 kb on + strand, within HSERO_RS00055 at 18.909 kb on + strand, within HSERO_RS00055 at 18.919 kb on - strand, within HSERO_RS00055 at 19.029 kb on + strand at 19.048 kb on - strand at 19.116 kb on + strand at 19.117 kb on - strand at 19.148 kb on + strand at 19.190 kb on + strand at 19.226 kb on - strand at 19.245 kb on + strand at 19.257 kb on - strand at 19.281 kb on + strand at 19.289 kb on - strand at 19.289 kb on - strand at 19.321 kb on + strand at 19.321 kb on + strand at 19.336 kb on - strand at 19.405 kb on - strand at 19.406 kb on - strand at 19.445 kb on - strand at 19.468 kb on + strand at 19.468 kb on + strand at 19.475 kb on + strand at 19.493 kb on - strand at 19.514 kb on - strand at 19.538 kb on + strand at 19.754 kb on - strand, within HSERO_RS00060 at 19.781 kb on + strand, within HSERO_RS00060 at 20.310 kb on + strand, within HSERO_RS00060 at 20.365 kb on + strand at 20.399 kb on + strand at 20.433 kb on - strand at 20.440 kb on - strand at 20.450 kb on - strand at 20.510 kb on + strand at 20.516 kb on + strand at 20.553 kb on + strand at 20.628 kb on - strand at 20.653 kb on - strand at 20.656 kb on + strand at 20.677 kb on - strand at 20.747 kb on + strand, within HSERO_RS00065 at 20.781 kb on - strand, within HSERO_RS00065 at 21.032 kb on + strand, within HSERO_RS00065 at 21.032 kb on + strand, within HSERO_RS00065 at 21.034 kb on + strand, within HSERO_RS00065 at 21.298 kb on + strand, within HSERO_RS00065 at 21.301 kb on - strand, within HSERO_RS00065
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 18,513 - HSERO_RS00055 0.58 -0.1 18,558 + HSERO_RS00055 0.61 +0.4 18,593 + HSERO_RS00055 0.64 +0.4 18,683 - HSERO_RS00055 0.70 -0.5 18,685 + HSERO_RS00055 0.70 +0.2 18,714 + HSERO_RS00055 0.72 -1.0 18,714 + HSERO_RS00055 0.72 -2.0 18,727 - HSERO_RS00055 0.73 -0.1 18,823 + HSERO_RS00055 0.80 -0.4 18,889 + HSERO_RS00055 0.84 +0.2 18,900 - HSERO_RS00055 0.85 +0.3 18,909 + HSERO_RS00055 0.86 +0.2 18,909 + HSERO_RS00055 0.86 -0.1 18,919 - HSERO_RS00055 0.86 +0.3 19,029 + +2.0 19,048 - +0.5 19,116 + +0.5 19,117 - -0.4 19,148 + -0.4 19,190 + +0.4 19,226 - +0.2 19,245 + +0.3 19,257 - +0.5 19,281 + +0.9 19,289 - -0.9 19,289 - +0.8 19,321 + +0.4 19,321 + +0.7 19,336 - +0.2 19,405 - -1.2 19,406 - -1.0 19,445 - -0.1 19,468 + +0.3 19,468 + -0.1 19,475 + -0.2 19,493 - -0.7 19,514 - +0.1 19,538 + -0.1 19,754 - HSERO_RS00060 0.29 +0.3 19,781 + HSERO_RS00060 0.32 +1.0 20,310 + HSERO_RS00060 0.89 -0.0 20,365 + -0.3 20,399 + -1.0 20,433 - -0.2 20,440 - +0.6 20,450 - +0.4 20,510 + +1.5 20,516 + -0.1 20,553 + +0.2 20,628 - -0.1 20,653 - +0.1 20,656 + +0.4 20,677 - +0.4 20,747 + HSERO_RS00065 0.13 +0.3 20,781 - HSERO_RS00065 0.14 -0.1 21,032 + HSERO_RS00065 0.25 -1.2 21,032 + HSERO_RS00065 0.25 -0.5 21,034 + HSERO_RS00065 0.25 -0.0 21,298 + HSERO_RS00065 0.36 -2.2 21,301 - HSERO_RS00065 0.36 +0.4
Or see this region's nucleotide sequence