Experiment: MoYLS4 with 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt frdB and DvMF_2461 are separated by 306 nucleotides DvMF_2461 and DvMF_2462 are separated by 314 nucleotides DvMF_2462 and DvMF_2463 are separated by 44 nucleotides
DvMF_2460: frdB - fumarate reductase iron-sulfur subunit (RefSeq), at 3,103,544 to 3,104,287
frdB
DvMF_2461: DvMF_2461 - fumarate hydratase (RefSeq), at 3,104,594 to 3,105,433
_2461
DvMF_2462: DvMF_2462 - hydro-lyase, Fe-S type, tartrate/fumarate subfamily, beta subunit (RefSeq), at 3,105,748 to 3,106,275
_2462
DvMF_2463: DvMF_2463 - hypothetical protein (RefSeq), at 3,106,320 to 3,107,615
_2463
Position (kb)
3104
3105
3106 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3103.679 kb on + strand, within frdB at 3103.819 kb on - strand, within frdB at 3104.071 kb on - strand, within frdB at 3104.241 kb on + strand at 3104.249 kb on - strand at 3104.435 kb on + strand at 3104.856 kb on - strand, within DvMF_2461 at 3104.944 kb on + strand, within DvMF_2461 at 3105.343 kb on - strand, within DvMF_2461 at 3105.348 kb on + strand, within DvMF_2461 at 3105.555 kb on + strand at 3105.767 kb on + strand at 3105.834 kb on + strand, within DvMF_2462 at 3105.834 kb on + strand, within DvMF_2462 at 3105.842 kb on - strand, within DvMF_2462 at 3105.842 kb on - strand, within DvMF_2462 at 3105.842 kb on - strand, within DvMF_2462 at 3105.868 kb on - strand, within DvMF_2462 at 3105.902 kb on + strand, within DvMF_2462 at 3105.972 kb on - strand, within DvMF_2462 at 3106.013 kb on - strand, within DvMF_2462 at 3106.085 kb on + strand, within DvMF_2462 at 3106.093 kb on - strand, within DvMF_2462 at 3106.311 kb on + strand at 3106.336 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with 5-Hydroxymethylfurfural 10 mM remove 3,103,679 + frdB DvMF_2460 0.18 -0.7 3,103,819 - frdB DvMF_2460 0.37 -1.2 3,104,071 - frdB DvMF_2460 0.71 -0.1 3,104,241 + -1.0 3,104,249 - -0.8 3,104,435 + +0.2 3,104,856 - DvMF_2461 0.31 -1.0 3,104,944 + DvMF_2461 0.42 -1.1 3,105,343 - DvMF_2461 0.89 -3.7 3,105,348 + DvMF_2461 0.90 -0.4 3,105,555 + -0.9 3,105,767 + -0.3 3,105,834 + DvMF_2462 0.16 -0.1 3,105,834 + DvMF_2462 0.16 +0.5 3,105,842 - DvMF_2462 0.18 -0.5 3,105,842 - DvMF_2462 0.18 -0.9 3,105,842 - DvMF_2462 0.18 +0.0 3,105,868 - DvMF_2462 0.23 -1.4 3,105,902 + DvMF_2462 0.29 -0.0 3,105,972 - DvMF_2462 0.42 -1.4 3,106,013 - DvMF_2462 0.50 -0.7 3,106,085 + DvMF_2462 0.64 -0.6 3,106,093 - DvMF_2462 0.65 -0.8 3,106,311 + +1.4 3,106,336 + -0.3
Or see this region's nucleotide sequence