Experiment: MoYLS4 with 5-Hydroxymethylfurfural 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1006 and DvMF_1007 overlap by 4 nucleotides DvMF_1007 and DvMF_1008 are separated by 26 nucleotides DvMF_1008 and DvMF_1009 overlap by 4 nucleotides DvMF_1009 and DvMF_1010 are separated by 171 nucleotides
DvMF_1006: DvMF_1006 - Beta-ketoacyl synthase (RefSeq), at 1,220,843 to 1,222,219
_1006
DvMF_1007: DvMF_1007 - hypothetical protein (RefSeq), at 1,222,216 to 1,222,743
_1007
DvMF_1008: DvMF_1008 - aminotransferase class I and II (RefSeq), at 1,222,770 to 1,224,110
_1008
DvMF_1009: DvMF_1009 - dethiobiotin synthase (RefSeq), at 1,224,107 to 1,224,850
_1009
DvMF_1010: DvMF_1010 - diaminopimelate decarboxylase (RefSeq), at 1,225,022 to 1,226,287
_1010
Position (kb)
1222
1223
1224
1225 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1221.985 kb on + strand, within DvMF_1006 at 1222.042 kb on + strand, within DvMF_1006 at 1222.113 kb on - strand at 1222.493 kb on - strand, within DvMF_1007 at 1222.626 kb on + strand, within DvMF_1007 at 1222.867 kb on + strand at 1223.206 kb on - strand, within DvMF_1008 at 1223.262 kb on + strand, within DvMF_1008 at 1223.606 kb on + strand, within DvMF_1008 at 1223.942 kb on + strand, within DvMF_1008 at 1223.950 kb on - strand, within DvMF_1008 at 1224.000 kb on - strand at 1224.042 kb on + strand at 1224.309 kb on + strand, within DvMF_1009 at 1224.364 kb on + strand, within DvMF_1009 at 1224.443 kb on - strand, within DvMF_1009 at 1224.572 kb on + strand, within DvMF_1009 at 1224.575 kb on - strand, within DvMF_1009 at 1224.685 kb on - strand, within DvMF_1009 at 1224.721 kb on + strand, within DvMF_1009 at 1224.858 kb on + strand at 1224.878 kb on + strand at 1224.880 kb on + strand at 1224.888 kb on - strand at 1224.888 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with 5-Hydroxymethylfurfural 10 mM remove 1,221,985 + DvMF_1006 0.83 +0.2 1,222,042 + DvMF_1006 0.87 +0.2 1,222,113 - -0.2 1,222,493 - DvMF_1007 0.52 -0.7 1,222,626 + DvMF_1007 0.78 -0.0 1,222,867 + +0.7 1,223,206 - DvMF_1008 0.33 -0.8 1,223,262 + DvMF_1008 0.37 -1.1 1,223,606 + DvMF_1008 0.62 -3.0 1,223,942 + DvMF_1008 0.87 -1.5 1,223,950 - DvMF_1008 0.88 -0.4 1,224,000 - -0.3 1,224,042 + -0.5 1,224,309 + DvMF_1009 0.27 -0.9 1,224,364 + DvMF_1009 0.35 -0.2 1,224,443 - DvMF_1009 0.45 -0.6 1,224,572 + DvMF_1009 0.62 -1.9 1,224,575 - DvMF_1009 0.63 -1.6 1,224,685 - DvMF_1009 0.78 -0.7 1,224,721 + DvMF_1009 0.83 -0.9 1,224,858 + -0.6 1,224,878 + -0.5 1,224,880 + -0.7 1,224,888 - -0.2 1,224,888 - +0.0
Or see this region's nucleotide sequence