Experiment: Iron excess (2.5x) , H2 media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06875 and MMJJ_RS06880 are separated by 21 nucleotides MMJJ_RS06880 and MMJJ_RS06885 overlap by 1 nucleotides MMJJ_RS06885 and MMJJ_RS06890 are separated by 1 nucleotides MMJJ_RS06890 and MMJJ_RS06895 are separated by 8 nucleotides MMJJ_RS06895 and MMJJ_RS06900 are separated by 5 nucleotides MMJJ_RS06900 and MMJJ_RS06905 are separated by 0 nucleotides
MMJJ_RS06875: MMJJ_RS06875 - hypothetical protein, at 1,266,311 to 1,266,973
_RS06875
MMJJ_RS06880: MMJJ_RS06880 - EhaG family protein, at 1,266,995 to 1,267,681
_RS06880
MMJJ_RS06885: MMJJ_RS06885 - EhaF family protein, at 1,267,681 to 1,268,154
_RS06885
MMJJ_RS06890: MMJJ_RS06890 - EhaE family protein, at 1,268,156 to 1,268,410
_RS06890
MMJJ_RS06895: MMJJ_RS06895 - DUF2108 domain-containing protein, at 1,268,419 to 1,268,697
_RS06895
MMJJ_RS06900: MMJJ_RS06900 - DUF2109 domain-containing protein, at 1,268,703 to 1,268,969
_RS06900
MMJJ_RS06905: MMJJ_RS06905 - hypothetical protein, at 1,268,970 to 1,269,461
_RS06905
Position (kb)
1267
1268
1269 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1266.741 kb on + strand, within MMJJ_RS06875 at 1266.751 kb on - strand, within MMJJ_RS06875 at 1266.752 kb on + strand, within MMJJ_RS06875 at 1266.888 kb on + strand, within MMJJ_RS06875 at 1266.905 kb on - strand, within MMJJ_RS06875 at 1266.909 kb on - strand at 1266.914 kb on - strand at 1266.959 kb on + strand at 1266.960 kb on - strand at 1267.224 kb on - strand, within MMJJ_RS06880 at 1267.557 kb on + strand, within MMJJ_RS06880 at 1267.583 kb on + strand, within MMJJ_RS06880 at 1267.781 kb on - strand at 1267.782 kb on + strand, within MMJJ_RS06885 at 1267.782 kb on + strand, within MMJJ_RS06885 at 1267.785 kb on + strand at 1267.932 kb on - strand, within MMJJ_RS06885 at 1267.951 kb on - strand at 1267.952 kb on + strand at 1268.075 kb on + strand, within MMJJ_RS06885 at 1268.075 kb on + strand, within MMJJ_RS06885 at 1268.206 kb on - strand, within MMJJ_RS06890 at 1268.236 kb on - strand, within MMJJ_RS06890 at 1268.236 kb on - strand, within MMJJ_RS06890 at 1268.256 kb on - strand, within MMJJ_RS06890 at 1268.268 kb on + strand, within MMJJ_RS06890 at 1268.424 kb on - strand at 1268.427 kb on - strand at 1268.471 kb on + strand, within MMJJ_RS06895 at 1268.475 kb on + strand, within MMJJ_RS06895 at 1268.480 kb on - strand at 1268.791 kb on - strand, within MMJJ_RS06900 at 1268.861 kb on - strand at 1268.972 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Iron excess (2.5x) , H2 media remove 1,266,741 + MMJJ_RS06875 0.65 +0.1 1,266,751 - MMJJ_RS06875 0.66 -1.4 1,266,752 + MMJJ_RS06875 0.67 -2.2 1,266,888 + MMJJ_RS06875 0.87 -2.7 1,266,905 - MMJJ_RS06875 0.90 -3.9 1,266,909 - -0.0 1,266,914 - -2.2 1,266,959 + -1.3 1,266,960 - -1.2 1,267,224 - MMJJ_RS06880 0.33 -1.9 1,267,557 + MMJJ_RS06880 0.82 -1.3 1,267,583 + MMJJ_RS06880 0.86 -0.6 1,267,781 - -0.0 1,267,782 + MMJJ_RS06885 0.21 +0.3 1,267,782 + MMJJ_RS06885 0.21 -1.4 1,267,785 + -0.0 1,267,932 - MMJJ_RS06885 0.53 -1.8 1,267,951 - -0.0 1,267,952 + -1.0 1,268,075 + MMJJ_RS06885 0.83 -1.4 1,268,075 + MMJJ_RS06885 0.83 -1.6 1,268,206 - MMJJ_RS06890 0.20 -0.8 1,268,236 - MMJJ_RS06890 0.31 -0.8 1,268,236 - MMJJ_RS06890 0.31 -0.8 1,268,256 - MMJJ_RS06890 0.39 -1.8 1,268,268 + MMJJ_RS06890 0.44 -0.8 1,268,424 - -2.3 1,268,427 - -0.8 1,268,471 + MMJJ_RS06895 0.19 -2.0 1,268,475 + MMJJ_RS06895 0.20 -1.4 1,268,480 - -1.2 1,268,791 - MMJJ_RS06900 0.33 -1.8 1,268,861 - -0.4 1,268,972 + -1.0
Or see this region's nucleotide sequence