Experiment: Iron excess (2.5x) , H2 media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06050 and MMJJ_RS06055 are separated by 140 nucleotides MMJJ_RS06055 and MMJJ_RS06060 are separated by 194 nucleotides
MMJJ_RS06050: MMJJ_RS06050 - molybdopterin molybdotransferase MoeA, at 1,123,271 to 1,124,479
_RS06050
MMJJ_RS06055: MMJJ_RS06055 - S-methyl-5-thioribose-1-phosphate isomerase, at 1,124,620 to 1,125,585
_RS06055
MMJJ_RS06060: MMJJ_RS06060 - aspartate--tRNA(Asn) ligase, at 1,125,780 to 1,127,096
_RS06060
Position (kb) 1124
1125
1126 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 1123.635 kb on + strand, within MMJJ_RS06050 at 1123.673 kb on + strand, within MMJJ_RS06050 at 1123.676 kb on - strand, within MMJJ_RS06050 at 1123.724 kb on + strand, within MMJJ_RS06050 at 1123.757 kb on - strand, within MMJJ_RS06050 at 1123.798 kb on - strand, within MMJJ_RS06050 at 1123.798 kb on - strand, within MMJJ_RS06050 at 1123.849 kb on - strand, within MMJJ_RS06050 at 1123.920 kb on + strand, within MMJJ_RS06050 at 1123.959 kb on - strand, within MMJJ_RS06050 at 1124.033 kb on + strand, within MMJJ_RS06050 at 1124.039 kb on + strand, within MMJJ_RS06050 at 1124.103 kb on - strand, within MMJJ_RS06050 at 1124.103 kb on - strand, within MMJJ_RS06050 at 1124.104 kb on + strand, within MMJJ_RS06050 at 1124.131 kb on - strand, within MMJJ_RS06050 at 1124.136 kb on - strand, within MMJJ_RS06050 at 1124.137 kb on + strand, within MMJJ_RS06050 at 1124.227 kb on - strand, within MMJJ_RS06050 at 1124.227 kb on - strand, within MMJJ_RS06050 at 1124.231 kb on - strand, within MMJJ_RS06050 at 1124.272 kb on - strand, within MMJJ_RS06050 at 1124.273 kb on + strand, within MMJJ_RS06050 at 1124.375 kb on + strand at 1124.379 kb on + strand at 1124.408 kb on - strand at 1124.468 kb on - strand at 1124.515 kb on - strand at 1124.515 kb on - strand at 1124.515 kb on - strand at 1124.529 kb on - strand at 1124.529 kb on - strand at 1124.529 kb on - strand at 1124.529 kb on - strand at 1124.554 kb on - strand at 1124.559 kb on - strand at 1124.560 kb on + strand at 1124.560 kb on + strand at 1124.581 kb on - strand at 1124.582 kb on + strand at 1124.590 kb on - strand at 1124.590 kb on - strand at 1124.591 kb on + strand at 1124.601 kb on - strand at 1124.601 kb on - strand at 1124.601 kb on - strand at 1124.605 kb on - strand at 1124.606 kb on + strand at 1124.606 kb on + strand at 1124.672 kb on - strand at 1124.672 kb on - strand at 1124.708 kb on - strand at 1124.813 kb on - strand, within MMJJ_RS06055 at 1124.848 kb on - strand, within MMJJ_RS06055 at 1124.976 kb on + strand, within MMJJ_RS06055 at 1125.030 kb on - strand, within MMJJ_RS06055 at 1125.033 kb on - strand, within MMJJ_RS06055 at 1125.034 kb on + strand, within MMJJ_RS06055 at 1125.048 kb on - strand, within MMJJ_RS06055 at 1125.090 kb on + strand, within MMJJ_RS06055 at 1125.099 kb on - strand, within MMJJ_RS06055 at 1125.100 kb on + strand, within MMJJ_RS06055 at 1125.112 kb on - strand, within MMJJ_RS06055 at 1125.113 kb on + strand, within MMJJ_RS06055 at 1125.113 kb on + strand, within MMJJ_RS06055 at 1125.134 kb on - strand, within MMJJ_RS06055 at 1125.134 kb on - strand, within MMJJ_RS06055 at 1125.134 kb on - strand, within MMJJ_RS06055 at 1125.134 kb on - strand, within MMJJ_RS06055 at 1125.135 kb on + strand, within MMJJ_RS06055 at 1125.135 kb on + strand, within MMJJ_RS06055 at 1125.138 kb on + strand, within MMJJ_RS06055 at 1125.138 kb on + strand, within MMJJ_RS06055 at 1125.212 kb on - strand, within MMJJ_RS06055 at 1125.213 kb on + strand, within MMJJ_RS06055 at 1125.273 kb on + strand, within MMJJ_RS06055 at 1125.276 kb on + strand, within MMJJ_RS06055 at 1125.345 kb on + strand, within MMJJ_RS06055 at 1125.388 kb on - strand, within MMJJ_RS06055 at 1125.393 kb on - strand, within MMJJ_RS06055 at 1125.394 kb on + strand, within MMJJ_RS06055 at 1125.423 kb on - strand, within MMJJ_RS06055 at 1125.423 kb on - strand, within MMJJ_RS06055 at 1125.424 kb on + strand, within MMJJ_RS06055 at 1125.424 kb on + strand, within MMJJ_RS06055 at 1125.462 kb on - strand, within MMJJ_RS06055 at 1125.462 kb on - strand, within MMJJ_RS06055 at 1125.468 kb on - strand, within MMJJ_RS06055 at 1125.473 kb on - strand, within MMJJ_RS06055 at 1125.473 kb on - strand, within MMJJ_RS06055 at 1125.503 kb on + strand at 1125.540 kb on - strand at 1125.567 kb on + strand at 1125.579 kb on - strand at 1125.579 kb on - strand at 1125.579 kb on - strand at 1125.584 kb on + strand at 1125.613 kb on + strand at 1125.643 kb on - strand at 1125.644 kb on + strand at 1125.648 kb on - strand at 1125.649 kb on + strand at 1125.653 kb on - strand at 1125.653 kb on - strand at 1125.653 kb on - strand at 1125.653 kb on - strand at 1125.664 kb on + strand at 1125.668 kb on - strand at 1125.668 kb on - strand at 1125.669 kb on + strand at 1125.669 kb on + strand at 1125.670 kb on - strand at 1125.670 kb on - strand at 1125.670 kb on - strand at 1125.670 kb on - strand at 1125.670 kb on - strand at 1125.673 kb on - strand at 1125.674 kb on + strand at 1125.674 kb on + strand at 1125.682 kb on - strand at 1125.682 kb on - strand at 1125.682 kb on - strand at 1125.682 kb on - strand at 1125.687 kb on - strand at 1125.688 kb on + strand at 1125.688 kb on + strand at 1125.688 kb on + strand at 1125.688 kb on + strand at 1125.688 kb on + strand at 1125.688 kb on + strand at 1125.688 kb on + strand at 1125.688 kb on + strand at 1125.692 kb on - strand at 1125.693 kb on + strand at 1125.699 kb on - strand at 1125.699 kb on - strand at 1125.699 kb on - strand at 1125.700 kb on + strand at 1125.700 kb on + strand at 1125.700 kb on + strand at 1125.700 kb on + strand at 1125.700 kb on + strand at 1125.755 kb on - strand at 1125.769 kb on + strand at 1126.289 kb on + strand, within MMJJ_RS06060
Per-strain Table
Position Strand Gene LocusTag Fraction Iron excess (2.5x) , H2 media remove 1,123,635 + MMJJ_RS06050 0.30 -1.5 1,123,673 + MMJJ_RS06050 0.33 -3.8 1,123,676 - MMJJ_RS06050 0.33 -0.9 1,123,724 + MMJJ_RS06050 0.37 -2.5 1,123,757 - MMJJ_RS06050 0.40 -2.9 1,123,798 - MMJJ_RS06050 0.44 -2.4 1,123,798 - MMJJ_RS06050 0.44 -0.2 1,123,849 - MMJJ_RS06050 0.48 -2.0 1,123,920 + MMJJ_RS06050 0.54 -3.8 1,123,959 - MMJJ_RS06050 0.57 -3.5 1,124,033 + MMJJ_RS06050 0.63 -3.6 1,124,039 + MMJJ_RS06050 0.64 +0.1 1,124,103 - MMJJ_RS06050 0.69 -0.9 1,124,103 - MMJJ_RS06050 0.69 -4.3 1,124,104 + MMJJ_RS06050 0.69 -3.0 1,124,131 - MMJJ_RS06050 0.71 -1.8 1,124,136 - MMJJ_RS06050 0.72 -3.4 1,124,137 + MMJJ_RS06050 0.72 -0.7 1,124,227 - MMJJ_RS06050 0.79 -1.7 1,124,227 - MMJJ_RS06050 0.79 -3.5 1,124,231 - MMJJ_RS06050 0.79 -0.5 1,124,272 - MMJJ_RS06050 0.83 -2.3 1,124,273 + MMJJ_RS06050 0.83 -4.6 1,124,375 + -3.6 1,124,379 + -1.4 1,124,408 - -1.6 1,124,468 - +1.2 1,124,515 - +0.2 1,124,515 - -0.2 1,124,515 - -0.0 1,124,529 - +0.1 1,124,529 - +0.9 1,124,529 - +1.7 1,124,529 - +0.5 1,124,554 - +0.7 1,124,559 - +2.2 1,124,560 + -0.6 1,124,560 + -0.4 1,124,581 - -0.8 1,124,582 + -0.7 1,124,590 - +0.8 1,124,590 - +1.2 1,124,591 + -0.7 1,124,601 - +0.2 1,124,601 - +1.4 1,124,601 - +0.6 1,124,605 - -0.5 1,124,606 + +1.0 1,124,606 + -1.1 1,124,672 - +1.1 1,124,672 - -2.3 1,124,708 - +1.8 1,124,813 - MMJJ_RS06055 0.20 +0.1 1,124,848 - MMJJ_RS06055 0.24 +0.8 1,124,976 + MMJJ_RS06055 0.37 -1.8 1,125,030 - MMJJ_RS06055 0.42 +0.1 1,125,033 - MMJJ_RS06055 0.43 -0.3 1,125,034 + MMJJ_RS06055 0.43 +1.7 1,125,048 - MMJJ_RS06055 0.44 +0.3 1,125,090 + MMJJ_RS06055 0.49 -0.6 1,125,099 - MMJJ_RS06055 0.50 +0.1 1,125,100 + MMJJ_RS06055 0.50 +1.1 1,125,112 - MMJJ_RS06055 0.51 -0.1 1,125,113 + MMJJ_RS06055 0.51 +1.5 1,125,113 + MMJJ_RS06055 0.51 -1.1 1,125,134 - MMJJ_RS06055 0.53 -1.5 1,125,134 - MMJJ_RS06055 0.53 -0.8 1,125,134 - MMJJ_RS06055 0.53 -1.4 1,125,134 - MMJJ_RS06055 0.53 +0.9 1,125,135 + MMJJ_RS06055 0.53 +1.3 1,125,135 + MMJJ_RS06055 0.53 -0.6 1,125,138 + MMJJ_RS06055 0.54 +0.5 1,125,138 + MMJJ_RS06055 0.54 +0.6 1,125,212 - MMJJ_RS06055 0.61 +0.0 1,125,213 + MMJJ_RS06055 0.61 +0.6 1,125,273 + MMJJ_RS06055 0.68 +0.1 1,125,276 + MMJJ_RS06055 0.68 +2.8 1,125,345 + MMJJ_RS06055 0.75 +0.8 1,125,388 - MMJJ_RS06055 0.80 +0.7 1,125,393 - MMJJ_RS06055 0.80 +1.3 1,125,394 + MMJJ_RS06055 0.80 +1.0 1,125,423 - MMJJ_RS06055 0.83 +3.2 1,125,423 - MMJJ_RS06055 0.83 +0.1 1,125,424 + MMJJ_RS06055 0.83 +0.5 1,125,424 + MMJJ_RS06055 0.83 +0.8 1,125,462 - MMJJ_RS06055 0.87 +0.5 1,125,462 - MMJJ_RS06055 0.87 +2.1 1,125,468 - MMJJ_RS06055 0.88 -1.2 1,125,473 - MMJJ_RS06055 0.88 +0.3 1,125,473 - MMJJ_RS06055 0.88 -0.1 1,125,503 + +0.5 1,125,540 - -0.2 1,125,567 + +1.0 1,125,579 - -0.4 1,125,579 - -0.1 1,125,579 - -0.3 1,125,584 + +1.1 1,125,613 + -0.8 1,125,643 - +1.5 1,125,644 + +0.9 1,125,648 - +0.4 1,125,649 + -3.1 1,125,653 - +0.8 1,125,653 - +1.3 1,125,653 - +0.7 1,125,653 - -0.3 1,125,664 + +2.4 1,125,668 - +0.8 1,125,668 - +1.0 1,125,669 + +1.2 1,125,669 + -1.1 1,125,670 - -0.2 1,125,670 - +1.2 1,125,670 - +0.9 1,125,670 - +0.2 1,125,670 - +0.1 1,125,673 - -2.4 1,125,674 + +0.6 1,125,674 + +5.1 1,125,682 - +1.0 1,125,682 - +3.0 1,125,682 - -2.0 1,125,682 - +0.1 1,125,687 - -0.0 1,125,688 + -0.7 1,125,688 + +0.5 1,125,688 + -0.2 1,125,688 + +0.1 1,125,688 + +2.1 1,125,688 + +0.6 1,125,688 + +1.7 1,125,688 + +0.7 1,125,692 - +0.3 1,125,693 + -3.9 1,125,699 - +0.0 1,125,699 - +0.8 1,125,699 - +0.2 1,125,700 + +0.8 1,125,700 + +2.1 1,125,700 + +1.0 1,125,700 + +1.1 1,125,700 + +1.2 1,125,755 - -1.7 1,125,769 + +1.5 1,126,289 + MMJJ_RS06060 0.39 -1.1
Or see this region's nucleotide sequence