Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS16295 and HSERO_RS16300 are separated by 264 nucleotides HSERO_RS16300 and HSERO_RS16305 are separated by 132 nucleotides HSERO_RS16305 and HSERO_RS16310 are separated by 229 nucleotides
HSERO_RS16295: HSERO_RS16295 - LysR family transcriptional regulator, at 3,725,660 to 3,726,604
_RS16295
HSERO_RS16300: HSERO_RS16300 - peptidase M20, at 3,726,869 to 3,728,632
_RS16300
HSERO_RS16305: HSERO_RS16305 - hypothetical protein, at 3,728,765 to 3,729,154
_RS16305
HSERO_RS16310: HSERO_RS16310 - hydroxydechloroatrazine ethylaminohydrolase, at 3,729,384 to 3,730,760
_RS16310
Position (kb)
3726
3727
3728
3729 Strain fitness (log2 ratio)
-1
0
1 at 3725.926 kb on + strand, within HSERO_RS16295 at 3726.174 kb on - strand, within HSERO_RS16295 at 3726.260 kb on - strand, within HSERO_RS16295 at 3726.516 kb on - strand at 3726.613 kb on - strand at 3726.939 kb on - strand at 3727.138 kb on + strand, within HSERO_RS16300 at 3727.154 kb on + strand, within HSERO_RS16300 at 3727.154 kb on + strand, within HSERO_RS16300 at 3727.455 kb on - strand, within HSERO_RS16300 at 3727.471 kb on - strand, within HSERO_RS16300 at 3727.478 kb on - strand, within HSERO_RS16300 at 3727.501 kb on - strand, within HSERO_RS16300 at 3727.841 kb on - strand, within HSERO_RS16300 at 3727.979 kb on - strand, within HSERO_RS16300 at 3728.133 kb on + strand, within HSERO_RS16300 at 3728.155 kb on + strand, within HSERO_RS16300 at 3728.168 kb on + strand, within HSERO_RS16300 at 3728.173 kb on + strand, within HSERO_RS16300 at 3728.352 kb on + strand, within HSERO_RS16300 at 3728.470 kb on + strand at 3728.482 kb on + strand at 3728.546 kb on - strand at 3728.593 kb on - strand at 3728.593 kb on - strand at 3728.697 kb on - strand at 3728.789 kb on + strand at 3728.844 kb on - strand, within HSERO_RS16305 at 3728.911 kb on + strand, within HSERO_RS16305 at 3728.933 kb on - strand, within HSERO_RS16305 at 3729.215 kb on + strand at 3729.223 kb on - strand at 3729.242 kb on + strand at 3729.266 kb on - strand at 3729.269 kb on + strand at 3729.382 kb on - strand at 3729.424 kb on - strand at 3729.426 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 3,725,926 + HSERO_RS16295 0.28 -0.1 3,726,174 - HSERO_RS16295 0.54 -0.4 3,726,260 - HSERO_RS16295 0.63 +1.7 3,726,516 - +0.3 3,726,613 - -0.0 3,726,939 - +1.2 3,727,138 + HSERO_RS16300 0.15 +0.5 3,727,154 + HSERO_RS16300 0.16 -0.5 3,727,154 + HSERO_RS16300 0.16 -0.8 3,727,455 - HSERO_RS16300 0.33 -0.0 3,727,471 - HSERO_RS16300 0.34 -0.6 3,727,478 - HSERO_RS16300 0.35 -0.2 3,727,501 - HSERO_RS16300 0.36 +0.9 3,727,841 - HSERO_RS16300 0.55 +0.4 3,727,979 - HSERO_RS16300 0.63 -0.2 3,728,133 + HSERO_RS16300 0.72 +1.0 3,728,155 + HSERO_RS16300 0.73 +1.1 3,728,168 + HSERO_RS16300 0.74 +0.7 3,728,173 + HSERO_RS16300 0.74 +0.7 3,728,352 + HSERO_RS16300 0.84 +0.4 3,728,470 + +0.2 3,728,482 + +0.4 3,728,546 - +0.2 3,728,593 - -1.7 3,728,593 - -0.6 3,728,697 - +0.3 3,728,789 + -0.2 3,728,844 - HSERO_RS16305 0.20 -0.2 3,728,911 + HSERO_RS16305 0.37 -0.4 3,728,933 - HSERO_RS16305 0.43 -1.3 3,729,215 + -0.7 3,729,223 - +0.1 3,729,242 + +0.0 3,729,266 - +0.1 3,729,269 + +0.5 3,729,382 - -0.0 3,729,424 - -0.6 3,729,426 + +0.3
Or see this region's nucleotide sequence