Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3067 and Dshi_3068 are separated by 302 nucleotides Dshi_3068 and Dshi_3069 are separated by 59 nucleotides Dshi_3069 and Dshi_3070 are separated by 95 nucleotides
Dshi_3067: Dshi_3067 - acetoacetyl-CoA reductase (RefSeq), at 3,235,779 to 3,236,501
_3067
Dshi_3068: Dshi_3068 - transcriptional regulator, LysR family (RefSeq), at 3,236,804 to 3,237,751
_3068
Dshi_3069: Dshi_3069 - protein of unknown function DUF465 (RefSeq), at 3,237,811 to 3,237,978
_3069
Dshi_3070: Dshi_3070 - methyltransferase small (RefSeq), at 3,238,074 to 3,238,844
_3070
Position (kb)
3236
3237
3238 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3235.855 kb on + strand, within Dshi_3067 at 3235.863 kb on - strand, within Dshi_3067 at 3235.930 kb on - strand, within Dshi_3067 at 3236.036 kb on - strand, within Dshi_3067 at 3236.065 kb on - strand, within Dshi_3067 at 3236.134 kb on - strand, within Dshi_3067 at 3236.238 kb on + strand, within Dshi_3067 at 3236.526 kb on - strand at 3236.645 kb on + strand at 3237.023 kb on + strand, within Dshi_3068 at 3237.132 kb on + strand, within Dshi_3068 at 3237.140 kb on - strand, within Dshi_3068 at 3237.201 kb on - strand, within Dshi_3068 at 3237.532 kb on + strand, within Dshi_3068 at 3237.598 kb on - strand, within Dshi_3068 at 3237.661 kb on - strand at 3237.755 kb on - strand at 3237.785 kb on - strand at 3237.827 kb on + strand at 3237.901 kb on + strand, within Dshi_3069 at 3237.939 kb on + strand, within Dshi_3069 at 3238.014 kb on + strand at 3238.409 kb on + strand, within Dshi_3070 at 3238.435 kb on - strand, within Dshi_3070 at 3238.454 kb on + strand, within Dshi_3070 at 3238.459 kb on + strand, within Dshi_3070 at 3238.494 kb on - strand, within Dshi_3070 at 3238.655 kb on + strand, within Dshi_3070 at 3238.655 kb on + strand, within Dshi_3070 at 3238.748 kb on + strand, within Dshi_3070
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 3,235,855 + Dshi_3067 0.11 -2.0 3,235,863 - Dshi_3067 0.12 -0.7 3,235,930 - Dshi_3067 0.21 -2.9 3,236,036 - Dshi_3067 0.36 -2.7 3,236,065 - Dshi_3067 0.40 -0.9 3,236,134 - Dshi_3067 0.49 -0.8 3,236,238 + Dshi_3067 0.63 -2.5 3,236,526 - +0.1 3,236,645 + -0.1 3,237,023 + Dshi_3068 0.23 -0.7 3,237,132 + Dshi_3068 0.35 +0.1 3,237,140 - Dshi_3068 0.35 -0.2 3,237,201 - Dshi_3068 0.42 -0.3 3,237,532 + Dshi_3068 0.77 +0.1 3,237,598 - Dshi_3068 0.84 -0.0 3,237,661 - +0.3 3,237,755 - -0.0 3,237,785 - -1.6 3,237,827 + +2.0 3,237,901 + Dshi_3069 0.54 +0.1 3,237,939 + Dshi_3069 0.76 -1.8 3,238,014 + -0.1 3,238,409 + Dshi_3070 0.43 -1.9 3,238,435 - Dshi_3070 0.47 -0.5 3,238,454 + Dshi_3070 0.49 -1.3 3,238,459 + Dshi_3070 0.50 -1.3 3,238,494 - Dshi_3070 0.54 -0.7 3,238,655 + Dshi_3070 0.75 -0.9 3,238,655 + Dshi_3070 0.75 -2.0 3,238,748 + Dshi_3070 0.87 -0.8
Or see this region's nucleotide sequence