Experiment: L-Methionine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0277 and Dshi_0278 overlap by 4 nucleotides Dshi_0278 and Dshi_0279 are separated by 95 nucleotides Dshi_0279 and Dshi_0280 are separated by 291 nucleotides Dshi_0280 and Dshi_0281 are separated by 163 nucleotides
Dshi_0277: Dshi_0277 - ribosomal protein L4/L1e (RefSeq), at 280,131 to 280,748
_0277
Dshi_0278: Dshi_0278 - Ribosomal protein L25/L23 (RefSeq), at 280,745 to 281,041
_0278
Dshi_0279: Dshi_0279 - hypothetical protein (RefSeq), at 281,137 to 281,487
_0279
Dshi_0280: Dshi_0280 - hypothetical protein (RefSeq), at 281,779 to 282,159
_0280
Dshi_0281: Dshi_0281 - YHS domain protein (RefSeq), at 282,323 to 282,814
_0281
Position (kb) 281
282 Strain fitness (log2 ratio) -1
0
1 at 281.108 kb on - strand at 281.415 kb on - strand, within Dshi_0279 at 281.891 kb on - strand, within Dshi_0280 at 281.904 kb on + strand, within Dshi_0280 at 282.017 kb on - strand, within Dshi_0280 at 282.137 kb on - strand at 282.166 kb on + strand at 282.194 kb on + strand at 282.200 kb on + strand at 282.231 kb on - strand at 282.231 kb on - strand at 282.242 kb on + strand at 282.270 kb on - strand at 282.316 kb on + strand at 282.321 kb on + strand at 282.321 kb on + strand at 282.329 kb on - strand at 282.403 kb on + strand, within Dshi_0281 at 282.403 kb on + strand, within Dshi_0281
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (N) remove 281,108 - +0.3 281,415 - Dshi_0279 0.79 +0.4 281,891 - Dshi_0280 0.29 -1.0 281,904 + Dshi_0280 0.33 -0.6 282,017 - Dshi_0280 0.62 -0.2 282,137 - -0.4 282,166 + -0.1 282,194 + +0.8 282,200 + -0.3 282,231 - -0.2 282,231 - -1.1 282,242 + -0.2 282,270 - +0.3 282,316 + +0.2 282,321 + -0.4 282,321 + +0.6 282,329 - +0.3 282,403 + Dshi_0281 0.16 -0.3 282,403 + Dshi_0281 0.16 -0.7
Or see this region's nucleotide sequence