Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS18655

Experiment: Growth at pH6 and (C) D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS18645 and PFLU_RS18650 are separated by 11 nucleotidesPFLU_RS18650 and PFLU_RS18655 are separated by 9 nucleotidesPFLU_RS18655 and PFLU_RS18660 are separated by 2 nucleotidesPFLU_RS18660 and PFLU_RS18665 overlap by 4 nucleotides PFLU_RS18645: PFLU_RS18645 - NADH-quinone oxidoreductase subunit NuoH, at 4,221,409 to 4,222,416 _RS18645 PFLU_RS18650: PFLU_RS18650 - NADH-quinone oxidoreductase subunit NuoI, at 4,222,428 to 4,222,976 _RS18650 PFLU_RS18655: PFLU_RS18655 - NADH-quinone oxidoreductase subunit J, at 4,222,986 to 4,223,489 _RS18655 PFLU_RS18660: PFLU_RS18660 - NADH-quinone oxidoreductase subunit NuoK, at 4,223,492 to 4,223,800 _RS18660 PFLU_RS18665: PFLU_RS18665 - NADH-quinone oxidoreductase subunit L, at 4,223,797 to 4,225,650 _RS18665 Position (kb) 4222 4223 4224Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4222.002 kb on + strand, within PFLU_RS18645at 4222.003 kb on - strand, within PFLU_RS18645at 4222.085 kb on - strand, within PFLU_RS18645at 4222.097 kb on - strand, within PFLU_RS18645at 4222.135 kb on + strand, within PFLU_RS18645at 4222.135 kb on + strand, within PFLU_RS18645at 4222.136 kb on - strand, within PFLU_RS18645at 4222.136 kb on - strand, within PFLU_RS18645at 4222.136 kb on - strand, within PFLU_RS18645at 4222.136 kb on - strand, within PFLU_RS18645at 4222.437 kb on + strandat 4222.438 kb on - strandat 4222.549 kb on - strand, within PFLU_RS18650at 4222.876 kb on - strand, within PFLU_RS18650at 4222.885 kb on - strand, within PFLU_RS18650at 4222.898 kb on + strand, within PFLU_RS18650at 4222.898 kb on + strand, within PFLU_RS18650at 4222.974 kb on + strandat 4222.974 kb on + strandat 4223.076 kb on + strand, within PFLU_RS18655at 4223.076 kb on + strand, within PFLU_RS18655at 4223.076 kb on + strand, within PFLU_RS18655at 4223.161 kb on - strand, within PFLU_RS18655at 4223.310 kb on + strand, within PFLU_RS18655at 4223.310 kb on + strand, within PFLU_RS18655at 4223.310 kb on + strand, within PFLU_RS18655at 4223.311 kb on - strand, within PFLU_RS18655at 4223.311 kb on - strand, within PFLU_RS18655at 4223.311 kb on - strand, within PFLU_RS18655at 4223.311 kb on - strand, within PFLU_RS18655at 4223.501 kb on - strandat 4223.747 kb on + strand, within PFLU_RS18660at 4223.936 kb on - strandat 4223.936 kb on - strandat 4224.040 kb on + strand, within PFLU_RS18665at 4224.118 kb on + strand, within PFLU_RS18665at 4224.119 kb on - strand, within PFLU_RS18665at 4224.119 kb on - strand, within PFLU_RS18665at 4224.119 kb on - strand, within PFLU_RS18665at 4224.119 kb on - strand, within PFLU_RS18665at 4224.139 kb on + strand, within PFLU_RS18665at 4224.157 kb on + strand, within PFLU_RS18665at 4224.157 kb on + strand, within PFLU_RS18665at 4224.157 kb on + strand, within PFLU_RS18665at 4224.253 kb on + strand, within PFLU_RS18665at 4224.253 kb on + strand, within PFLU_RS18665at 4224.254 kb on - strand, within PFLU_RS18665at 4224.274 kb on + strand, within PFLU_RS18665at 4224.275 kb on - strand, within PFLU_RS18665at 4224.275 kb on - strand, within PFLU_RS18665at 4224.275 kb on - strand, within PFLU_RS18665at 4224.306 kb on + strand, within PFLU_RS18665at 4224.307 kb on - strand, within PFLU_RS18665at 4224.316 kb on - strand, within PFLU_RS18665

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Per-strain Table

Position Strand Gene LocusTag Fraction Growth at pH6 and (C) D-Glucose
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4,222,002 + PFLU_RS18645 0.59 +0.7
4,222,003 - PFLU_RS18645 0.59 -1.0
4,222,085 - PFLU_RS18645 0.67 -0.6
4,222,097 - PFLU_RS18645 0.68 +0.0
4,222,135 + PFLU_RS18645 0.72 -0.3
4,222,135 + PFLU_RS18645 0.72 -1.0
4,222,136 - PFLU_RS18645 0.72 -1.6
4,222,136 - PFLU_RS18645 0.72 +0.0
4,222,136 - PFLU_RS18645 0.72 -1.3
4,222,136 - PFLU_RS18645 0.72 -1.6
4,222,437 + +1.0
4,222,438 - -1.6
4,222,549 - PFLU_RS18650 0.22 +1.0
4,222,876 - PFLU_RS18650 0.82 +1.0
4,222,885 - PFLU_RS18650 0.83 -0.6
4,222,898 + PFLU_RS18650 0.86 +0.6
4,222,898 + PFLU_RS18650 0.86 +0.6
4,222,974 + -1.6
4,222,974 + -2.6
4,223,076 + PFLU_RS18655 0.18 -1.0
4,223,076 + PFLU_RS18655 0.18 -0.6
4,223,076 + PFLU_RS18655 0.18 -0.6
4,223,161 - PFLU_RS18655 0.35 -1.3
4,223,310 + PFLU_RS18655 0.64 +1.0
4,223,310 + PFLU_RS18655 0.64 +1.0
4,223,310 + PFLU_RS18655 0.64 +0.0
4,223,311 - PFLU_RS18655 0.64 -0.6
4,223,311 - PFLU_RS18655 0.64 -1.0
4,223,311 - PFLU_RS18655 0.64 -0.2
4,223,311 - PFLU_RS18655 0.64 -0.6
4,223,501 - +0.0
4,223,747 + PFLU_RS18660 0.83 -0.6
4,223,936 - -2.0
4,223,936 - +2.0
4,224,040 + PFLU_RS18665 0.13 -0.6
4,224,118 + PFLU_RS18665 0.17 +3.3
4,224,119 - PFLU_RS18665 0.17 +1.0
4,224,119 - PFLU_RS18665 0.17 -0.6
4,224,119 - PFLU_RS18665 0.17 -0.6
4,224,119 - PFLU_RS18665 0.17 +0.0
4,224,139 + PFLU_RS18665 0.18 +0.0
4,224,157 + PFLU_RS18665 0.19 +0.0
4,224,157 + PFLU_RS18665 0.19 +0.0
4,224,157 + PFLU_RS18665 0.19 +3.3
4,224,253 + PFLU_RS18665 0.25 -0.6
4,224,253 + PFLU_RS18665 0.25 -0.6
4,224,254 - PFLU_RS18665 0.25 -0.6
4,224,274 + PFLU_RS18665 0.26 +1.0
4,224,275 - PFLU_RS18665 0.26 -0.8
4,224,275 - PFLU_RS18665 0.26 -2.0
4,224,275 - PFLU_RS18665 0.26 -0.6
4,224,306 + PFLU_RS18665 0.27 -1.0
4,224,307 - PFLU_RS18665 0.28 +0.0
4,224,316 - PFLU_RS18665 0.28 -1.0

Or see this region's nucleotide sequence