Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS04715

Experiment: Growth at pH6 and (C) D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS04710 and PFLU_RS04715 overlap by 4 nucleotidesPFLU_RS04715 and PFLU_RS04720 are separated by 3 nucleotidesPFLU_RS04720 and PFLU_RS04725 are separated by 15 nucleotides PFLU_RS04710: PFLU_RS04710 - UDP-N-acetylmuramate--L-alanine ligase, at 1,052,520 to 1,053,965 _RS04710 PFLU_RS04715: PFLU_RS04715 - D-alanine--D-alanine ligase, at 1,053,962 to 1,054,918 _RS04715 PFLU_RS04720: PFLU_RS04720 - cell division protein FtsQ/DivIB, at 1,054,922 to 1,055,791 _RS04720 PFLU_RS04725: PFLU_RS04725 - cell division protein FtsA, at 1,055,807 to 1,057,069 _RS04725 Position (kb) 1053 1054 1055Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1054.498 kb on + strand, within PFLU_RS04715at 1054.502 kb on + strand, within PFLU_RS04715at 1054.502 kb on + strand, within PFLU_RS04715at 1054.502 kb on + strand, within PFLU_RS04715at 1054.575 kb on + strand, within PFLU_RS04715at 1054.613 kb on + strand, within PFLU_RS04715at 1054.619 kb on + strand, within PFLU_RS04715at 1054.619 kb on + strand, within PFLU_RS04715at 1054.631 kb on + strand, within PFLU_RS04715at 1054.631 kb on + strand, within PFLU_RS04715at 1054.631 kb on + strand, within PFLU_RS04715at 1054.631 kb on + strand, within PFLU_RS04715at 1054.631 kb on + strand, within PFLU_RS04715at 1054.631 kb on + strand, within PFLU_RS04715at 1054.631 kb on + strand, within PFLU_RS04715at 1054.631 kb on + strand, within PFLU_RS04715at 1054.631 kb on + strand, within PFLU_RS04715at 1054.631 kb on + strand, within PFLU_RS04715at 1054.833 kb on + strandat 1054.833 kb on + strandat 1054.833 kb on + strandat 1054.833 kb on + strandat 1054.833 kb on + strandat 1054.833 kb on + strandat 1054.833 kb on + strandat 1054.837 kb on + strandat 1054.837 kb on + strandat 1054.837 kb on + strandat 1054.837 kb on + strandat 1054.916 kb on + strandat 1054.916 kb on + strandat 1054.916 kb on + strandat 1055.789 kb on + strandat 1055.789 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Growth at pH6 and (C) D-Glucose
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1,054,498 + PFLU_RS04715 0.56 -2.0
1,054,502 + PFLU_RS04715 0.56 -0.4
1,054,502 + PFLU_RS04715 0.56 -0.1
1,054,502 + PFLU_RS04715 0.56 -2.0
1,054,575 + PFLU_RS04715 0.64 -1.4
1,054,613 + PFLU_RS04715 0.68 -0.8
1,054,619 + PFLU_RS04715 0.69 +0.0
1,054,619 + PFLU_RS04715 0.69 -3.3
1,054,631 + PFLU_RS04715 0.70 -1.2
1,054,631 + PFLU_RS04715 0.70 -0.7
1,054,631 + PFLU_RS04715 0.70 -2.1
1,054,631 + PFLU_RS04715 0.70 -1.2
1,054,631 + PFLU_RS04715 0.70 -4.0
1,054,631 + PFLU_RS04715 0.70 -0.9
1,054,631 + PFLU_RS04715 0.70 -1.8
1,054,631 + PFLU_RS04715 0.70 -1.8
1,054,631 + PFLU_RS04715 0.70 -2.5
1,054,631 + PFLU_RS04715 0.70 -1.8
1,054,833 + -0.4
1,054,833 + +0.6
1,054,833 + -2.9
1,054,833 + -3.5
1,054,833 + -0.5
1,054,833 + -0.2
1,054,833 + -1.6
1,054,837 + -2.2
1,054,837 + -0.9
1,054,837 + -2.8
1,054,837 + -1.8
1,054,916 + -0.3
1,054,916 + -0.8
1,054,916 + +1.1
1,055,789 + -0.8
1,055,789 + -2.2

Or see this region's nucleotide sequence