Experiment: Growth at pH6 and (C) D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS01905 and PFLU_RS01910 overlap by 4 nucleotides PFLU_RS01910 and PFLU_RS01915 overlap by 1 nucleotides PFLU_RS01915 and PFLU_RS01920 are separated by 144 nucleotides
PFLU_RS01905: PFLU_RS01905 - ubiquinone biosynthesis regulatory protein kinase UbiB, at 427,935 to 429,539
_RS01905
PFLU_RS01910: PFLU_RS01910 - sterol-binding protein, at 429,536 to 430,159
_RS01910
PFLU_RS01915: PFLU_RS01915 - bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE, at 430,159 to 430,929
_RS01915
PFLU_RS01920: PFLU_RS01920 - hypothetical protein, at 431,074 to 431,349
_RS01920
Position (kb) 429
430
431 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 429.684 kb on + strand, within PFLU_RS01910 at 429.685 kb on - strand, within PFLU_RS01910 at 429.802 kb on - strand, within PFLU_RS01910 at 429.802 kb on - strand, within PFLU_RS01910 at 430.016 kb on - strand, within PFLU_RS01910 at 430.930 kb on - strand at 430.930 kb on - strand at 430.963 kb on + strand at 430.963 kb on + strand at 430.963 kb on + strand at 430.963 kb on + strand at 430.963 kb on + strand at 430.964 kb on - strand at 430.964 kb on - strand at 430.964 kb on - strand at 430.964 kb on - strand at 431.002 kb on + strand at 431.002 kb on + strand at 431.002 kb on + strand at 431.002 kb on + strand at 431.002 kb on + strand at 431.002 kb on + strand at 431.002 kb on + strand at 431.002 kb on + strand at 431.002 kb on + strand at 431.002 kb on + strand at 431.002 kb on + strand at 431.003 kb on - strand at 431.003 kb on - strand at 431.003 kb on - strand at 431.003 kb on - strand at 431.003 kb on - strand at 431.003 kb on - strand at 431.003 kb on - strand at 431.003 kb on - strand at 431.003 kb on - strand at 431.042 kb on + strand at 431.043 kb on - strand at 431.054 kb on + strand at 431.057 kb on - strand at 431.057 kb on - strand at 431.057 kb on - strand at 431.082 kb on + strand at 431.082 kb on + strand at 431.082 kb on + strand at 431.082 kb on + strand at 431.082 kb on + strand at 431.082 kb on + strand at 431.083 kb on - strand at 431.083 kb on - strand at 431.083 kb on - strand at 431.083 kb on - strand at 431.083 kb on - strand at 431.083 kb on - strand at 431.083 kb on - strand at 431.085 kb on - strand at 431.085 kb on - strand at 431.112 kb on + strand, within PFLU_RS01920
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH6 and (C) D-Glucose remove 429,684 + PFLU_RS01910 0.24 +0.2 429,685 - PFLU_RS01910 0.24 -0.7 429,802 - PFLU_RS01910 0.43 -1.1 429,802 - PFLU_RS01910 0.43 -1.4 430,016 - PFLU_RS01910 0.77 -0.6 430,930 - -2.2 430,930 - -0.2 430,963 + -0.5 430,963 + +0.6 430,963 + -0.4 430,963 + +1.5 430,963 + -0.1 430,964 - +0.2 430,964 - -1.1 430,964 - +1.2 430,964 - -1.8 431,002 + +0.2 431,002 + +0.5 431,002 + +0.1 431,002 + +0.1 431,002 + +0.5 431,002 + +0.6 431,002 + -0.2 431,002 + +2.3 431,002 + -0.3 431,002 + +0.9 431,002 + -1.4 431,003 - -1.8 431,003 - -1.1 431,003 - -0.9 431,003 - -0.4 431,003 - +1.5 431,003 - -3.2 431,003 - +1.9 431,003 - +0.2 431,003 - +0.9 431,042 + -0.2 431,043 - +0.1 431,054 + -0.1 431,057 - +0.5 431,057 - +1.2 431,057 - +0.4 431,082 + -0.4 431,082 + -1.2 431,082 + +0.8 431,082 + +1.3 431,082 + -0.2 431,082 + +0.8 431,083 - +0.1 431,083 - +0.0 431,083 - +1.9 431,083 - +0.5 431,083 - +0.1 431,083 - +1.0 431,083 - -0.7 431,085 - -2.1 431,085 - +1.0 431,112 + PFLU_RS01920 0.14 +1.1
Or see this region's nucleotide sequence