Experiment: Iron excess (2.5x) , H2 media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01180 and MMJJ_RS01185 are separated by 300 nucleotides MMJJ_RS01185 and MMJJ_RS01190 are separated by 335 nucleotides MMJJ_RS01190 and MMJJ_RS01195 are separated by 76 nucleotides MMJJ_RS01195 and MMJJ_RS01200 are separated by 24 nucleotides
MMJJ_RS01180: MMJJ_RS01180 - hypothetical protein, at 227,490 to 228,200
_RS01180
MMJJ_RS01185: MMJJ_RS01185 - tRNA-Ser, at 228,501 to 228,587
_RS01185
MMJJ_RS01190: MMJJ_RS01190 - hypothetical protein, at 228,923 to 229,852
_RS01190
MMJJ_RS01195: MMJJ_RS01195 - aspartate dehydrogenase, at 229,929 to 230,732
_RS01195
MMJJ_RS01200: MMJJ_RS01200 - PRC-barrel domain-containing protein, at 230,757 to 231,011
_RS01200
Position (kb) 228
229
230 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 227.938 kb on - strand, within MMJJ_RS01180 at 227.973 kb on - strand, within MMJJ_RS01180 at 227.974 kb on + strand, within MMJJ_RS01180 at 227.974 kb on + strand, within MMJJ_RS01180 at 227.974 kb on + strand, within MMJJ_RS01180 at 227.974 kb on + strand, within MMJJ_RS01180 at 227.983 kb on - strand, within MMJJ_RS01180 at 227.984 kb on + strand, within MMJJ_RS01180 at 227.986 kb on - strand, within MMJJ_RS01180 at 227.987 kb on + strand, within MMJJ_RS01180 at 228.019 kb on - strand, within MMJJ_RS01180 at 228.047 kb on - strand, within MMJJ_RS01180 at 228.047 kb on - strand, within MMJJ_RS01180 at 228.048 kb on + strand, within MMJJ_RS01180 at 228.048 kb on + strand, within MMJJ_RS01180 at 228.052 kb on + strand, within MMJJ_RS01180 at 228.058 kb on - strand, within MMJJ_RS01180 at 228.058 kb on - strand, within MMJJ_RS01180 at 228.059 kb on + strand, within MMJJ_RS01180 at 228.071 kb on - strand, within MMJJ_RS01180 at 228.094 kb on - strand, within MMJJ_RS01180 at 228.094 kb on - strand, within MMJJ_RS01180 at 228.094 kb on - strand, within MMJJ_RS01180 at 228.094 kb on - strand, within MMJJ_RS01180 at 228.095 kb on + strand, within MMJJ_RS01180 at 228.095 kb on + strand, within MMJJ_RS01180 at 228.095 kb on + strand, within MMJJ_RS01180 at 228.095 kb on + strand, within MMJJ_RS01180 at 228.101 kb on + strand, within MMJJ_RS01180 at 228.147 kb on + strand at 228.151 kb on - strand at 228.160 kb on - strand at 228.160 kb on - strand at 228.161 kb on + strand at 228.161 kb on + strand at 228.163 kb on - strand at 228.164 kb on + strand at 228.167 kb on - strand at 228.204 kb on - strand at 228.241 kb on - strand at 228.326 kb on + strand at 228.326 kb on + strand at 228.326 kb on + strand at 228.328 kb on - strand at 228.328 kb on - strand at 228.643 kb on + strand at 228.676 kb on + strand at 228.710 kb on - strand at 228.710 kb on - strand at 228.711 kb on + strand at 228.731 kb on - strand at 228.731 kb on - strand at 228.756 kb on + strand at 228.756 kb on + strand at 228.772 kb on + strand at 228.846 kb on + strand at 228.860 kb on - strand at 228.864 kb on - strand at 228.864 kb on - strand at 228.864 kb on - strand at 228.864 kb on - strand at 228.864 kb on - strand at 228.864 kb on - strand at 228.864 kb on - strand at 228.865 kb on + strand at 228.865 kb on + strand at 228.865 kb on + strand at 228.865 kb on + strand at 228.865 kb on + strand at 228.865 kb on + strand at 228.865 kb on + strand at 228.865 kb on + strand at 228.865 kb on + strand at 228.867 kb on - strand at 228.867 kb on - strand at 228.867 kb on - strand at 228.867 kb on - strand at 228.867 kb on - strand at 228.868 kb on + strand at 228.868 kb on + strand at 228.868 kb on + strand at 228.869 kb on - strand at 228.869 kb on - strand at 228.869 kb on - strand at 228.869 kb on - strand at 228.869 kb on - strand at 228.869 kb on - strand at 228.869 kb on - strand at 228.869 kb on - strand at 228.870 kb on + strand at 228.870 kb on + strand at 228.870 kb on + strand at 228.870 kb on + strand at 228.870 kb on + strand at 228.870 kb on + strand at 228.870 kb on + strand at 228.872 kb on + strand at 228.875 kb on - strand at 228.875 kb on - strand at 228.884 kb on + strand at 228.888 kb on - strand at 229.006 kb on - strand at 229.007 kb on + strand at 229.014 kb on + strand at 229.057 kb on - strand, within MMJJ_RS01190 at 229.091 kb on - strand, within MMJJ_RS01190 at 229.094 kb on + strand, within MMJJ_RS01190 at 229.098 kb on - strand, within MMJJ_RS01190 at 229.103 kb on + strand, within MMJJ_RS01190 at 229.103 kb on + strand at 229.113 kb on + strand, within MMJJ_RS01190 at 229.165 kb on - strand, within MMJJ_RS01190 at 229.198 kb on - strand, within MMJJ_RS01190 at 229.198 kb on - strand, within MMJJ_RS01190 at 229.199 kb on + strand, within MMJJ_RS01190 at 229.199 kb on + strand, within MMJJ_RS01190 at 229.199 kb on + strand, within MMJJ_RS01190 at 229.203 kb on - strand, within MMJJ_RS01190 at 229.204 kb on + strand, within MMJJ_RS01190 at 229.204 kb on + strand, within MMJJ_RS01190 at 229.204 kb on + strand, within MMJJ_RS01190 at 229.214 kb on - strand, within MMJJ_RS01190 at 229.243 kb on - strand, within MMJJ_RS01190 at 229.244 kb on + strand, within MMJJ_RS01190 at 229.290 kb on + strand, within MMJJ_RS01190 at 229.293 kb on - strand, within MMJJ_RS01190 at 229.294 kb on + strand, within MMJJ_RS01190 at 229.301 kb on + strand, within MMJJ_RS01190 at 229.315 kb on - strand, within MMJJ_RS01190 at 229.315 kb on - strand, within MMJJ_RS01190 at 229.316 kb on + strand, within MMJJ_RS01190 at 229.348 kb on - strand, within MMJJ_RS01190 at 229.454 kb on - strand, within MMJJ_RS01190 at 229.468 kb on - strand, within MMJJ_RS01190 at 229.495 kb on + strand, within MMJJ_RS01190 at 229.572 kb on - strand, within MMJJ_RS01190 at 229.572 kb on - strand, within MMJJ_RS01190 at 229.611 kb on - strand, within MMJJ_RS01190 at 229.649 kb on - strand, within MMJJ_RS01190 at 229.650 kb on + strand, within MMJJ_RS01190 at 229.753 kb on + strand, within MMJJ_RS01190 at 229.753 kb on + strand, within MMJJ_RS01190 at 229.796 kb on + strand at 229.845 kb on + strand at 229.850 kb on - strand at 229.854 kb on - strand at 229.855 kb on + strand at 229.855 kb on + strand at 229.868 kb on - strand at 229.869 kb on + strand at 229.930 kb on - strand at 229.931 kb on + strand at 230.736 kb on + strand at 230.771 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Iron excess (2.5x) , H2 media remove 227,938 - MMJJ_RS01180 0.63 -0.5 227,973 - MMJJ_RS01180 0.68 -0.7 227,974 + MMJJ_RS01180 0.68 +0.6 227,974 + MMJJ_RS01180 0.68 +0.8 227,974 + MMJJ_RS01180 0.68 -0.3 227,974 + MMJJ_RS01180 0.68 -0.8 227,983 - MMJJ_RS01180 0.69 +0.6 227,984 + MMJJ_RS01180 0.69 +0.2 227,986 - MMJJ_RS01180 0.70 +0.1 227,987 + MMJJ_RS01180 0.70 -0.5 228,019 - MMJJ_RS01180 0.74 +0.7 228,047 - MMJJ_RS01180 0.78 -1.4 228,047 - MMJJ_RS01180 0.78 -0.5 228,048 + MMJJ_RS01180 0.78 -0.3 228,048 + MMJJ_RS01180 0.78 +0.1 228,052 + MMJJ_RS01180 0.79 -0.0 228,058 - MMJJ_RS01180 0.80 +0.5 228,058 - MMJJ_RS01180 0.80 +0.3 228,059 + MMJJ_RS01180 0.80 -0.4 228,071 - MMJJ_RS01180 0.82 -0.1 228,094 - MMJJ_RS01180 0.85 +0.7 228,094 - MMJJ_RS01180 0.85 -0.0 228,094 - MMJJ_RS01180 0.85 +0.7 228,094 - MMJJ_RS01180 0.85 +0.3 228,095 + MMJJ_RS01180 0.85 +0.4 228,095 + MMJJ_RS01180 0.85 -2.8 228,095 + MMJJ_RS01180 0.85 -3.6 228,095 + MMJJ_RS01180 0.85 +0.8 228,101 + MMJJ_RS01180 0.86 +1.1 228,147 + +1.3 228,151 - -3.3 228,160 - +0.5 228,160 - +0.4 228,161 + +0.1 228,161 + -0.2 228,163 - -0.1 228,164 + +1.2 228,167 - -1.0 228,204 - +0.9 228,241 - -1.0 228,326 + -0.2 228,326 + -0.6 228,326 + -0.5 228,328 - +1.3 228,328 - -1.1 228,643 + -0.4 228,676 + +0.7 228,710 - +2.5 228,710 - +0.3 228,711 + -0.6 228,731 - +2.5 228,731 - -0.3 228,756 + -0.1 228,756 + -0.4 228,772 + +1.5 228,846 + +1.3 228,860 - -0.3 228,864 - -0.3 228,864 - +0.8 228,864 - -2.6 228,864 - -0.7 228,864 - -0.4 228,864 - -1.7 228,864 - +1.4 228,865 + +0.8 228,865 + +0.2 228,865 + +1.5 228,865 + -0.3 228,865 + +0.6 228,865 + -0.6 228,865 + +1.8 228,865 + +1.1 228,865 + +0.8 228,867 - +0.0 228,867 - +1.5 228,867 - -0.3 228,867 - -0.1 228,867 - -2.0 228,868 + -0.3 228,868 + +0.7 228,868 + -0.3 228,869 - +1.0 228,869 - -1.0 228,869 - -0.4 228,869 - -1.3 228,869 - -2.7 228,869 - +0.0 228,869 - -0.5 228,869 - -0.2 228,870 + -0.0 228,870 + +1.0 228,870 + +0.4 228,870 + +0.4 228,870 + +0.3 228,870 + -0.6 228,870 + -0.7 228,872 + -0.8 228,875 - +0.9 228,875 - -0.1 228,884 + -1.2 228,888 - -2.8 229,006 - -0.8 229,007 + +0.0 229,014 + +0.5 229,057 - MMJJ_RS01190 0.14 -0.7 229,091 - MMJJ_RS01190 0.18 +0.2 229,094 + MMJJ_RS01190 0.18 -0.9 229,098 - MMJJ_RS01190 0.19 -3.3 229,103 + MMJJ_RS01190 0.19 -0.0 229,103 + -1.0 229,113 + MMJJ_RS01190 0.20 -0.0 229,165 - MMJJ_RS01190 0.26 -0.4 229,198 - MMJJ_RS01190 0.30 +0.4 229,198 - MMJJ_RS01190 0.30 +0.1 229,199 + MMJJ_RS01190 0.30 +0.0 229,199 + MMJJ_RS01190 0.30 +0.6 229,199 + MMJJ_RS01190 0.30 -0.3 229,203 - MMJJ_RS01190 0.30 -0.7 229,204 + MMJJ_RS01190 0.30 +0.5 229,204 + MMJJ_RS01190 0.30 +0.2 229,204 + MMJJ_RS01190 0.30 -0.3 229,214 - MMJJ_RS01190 0.31 +0.8 229,243 - MMJJ_RS01190 0.34 -3.4 229,244 + MMJJ_RS01190 0.35 -0.5 229,290 + MMJJ_RS01190 0.39 -0.1 229,293 - MMJJ_RS01190 0.40 -0.9 229,294 + MMJJ_RS01190 0.40 -0.3 229,301 + MMJJ_RS01190 0.41 +0.8 229,315 - MMJJ_RS01190 0.42 -1.4 229,315 - MMJJ_RS01190 0.42 -1.3 229,316 + MMJJ_RS01190 0.42 -3.4 229,348 - MMJJ_RS01190 0.46 +0.2 229,454 - MMJJ_RS01190 0.57 -0.6 229,468 - MMJJ_RS01190 0.59 -1.0 229,495 + MMJJ_RS01190 0.62 +0.5 229,572 - MMJJ_RS01190 0.70 +0.7 229,572 - MMJJ_RS01190 0.70 -2.0 229,611 - MMJJ_RS01190 0.74 -0.9 229,649 - MMJJ_RS01190 0.78 +1.0 229,650 + MMJJ_RS01190 0.78 -0.2 229,753 + MMJJ_RS01190 0.89 -0.6 229,753 + MMJJ_RS01190 0.89 +0.4 229,796 + -0.4 229,845 + +0.7 229,850 - -0.0 229,854 - +0.3 229,855 + -0.4 229,855 + -0.8 229,868 - -0.3 229,869 + -1.5 229,930 - +0.7 229,931 + +0.5 230,736 + -1.6 230,771 - +3.0
Or see this region's nucleotide sequence