Experiment: R2A with sodium bromide 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS15135 and LRK54_RS15140 overlap by 14 nucleotides LRK54_RS15140 and LRK54_RS15145 are separated by 165 nucleotides
LRK54_RS15135: LRK54_RS15135 - bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase, at 3,341,308 to 3,342,801
_RS15135
LRK54_RS15140: LRK54_RS15140 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE, at 3,342,788 to 3,343,273
_RS15140
LRK54_RS15145: LRK54_RS15145 - N-acetylmuramoyl-L-alanine amidase, at 3,343,439 to 3,344,947
_RS15145
Position (kb)
3342
3343
3344 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3341.812 kb on - strand, within LRK54_RS15135 at 3341.886 kb on + strand, within LRK54_RS15135 at 3341.886 kb on + strand, within LRK54_RS15135 at 3341.909 kb on + strand, within LRK54_RS15135 at 3341.909 kb on + strand, within LRK54_RS15135 at 3341.909 kb on + strand, within LRK54_RS15135 at 3341.909 kb on + strand, within LRK54_RS15135 at 3341.909 kb on + strand, within LRK54_RS15135 at 3341.909 kb on + strand, within LRK54_RS15135 at 3341.910 kb on - strand, within LRK54_RS15135 at 3341.910 kb on - strand, within LRK54_RS15135 at 3341.910 kb on - strand, within LRK54_RS15135 at 3341.910 kb on - strand, within LRK54_RS15135 at 3341.910 kb on - strand, within LRK54_RS15135 at 3342.034 kb on + strand, within LRK54_RS15135 at 3342.034 kb on + strand, within LRK54_RS15135 at 3342.034 kb on + strand, within LRK54_RS15135 at 3342.034 kb on + strand, within LRK54_RS15135 at 3342.034 kb on + strand, within LRK54_RS15135 at 3342.034 kb on + strand, within LRK54_RS15135 at 3342.034 kb on + strand, within LRK54_RS15135 at 3342.034 kb on + strand, within LRK54_RS15135 at 3342.034 kb on + strand, within LRK54_RS15135 at 3342.064 kb on + strand, within LRK54_RS15135 at 3342.293 kb on + strand, within LRK54_RS15135 at 3342.293 kb on + strand, within LRK54_RS15135 at 3342.293 kb on + strand, within LRK54_RS15135 at 3342.293 kb on + strand, within LRK54_RS15135 at 3342.293 kb on + strand, within LRK54_RS15135 at 3342.293 kb on + strand, within LRK54_RS15135 at 3342.293 kb on + strand, within LRK54_RS15135 at 3342.293 kb on + strand, within LRK54_RS15135 at 3342.293 kb on + strand, within LRK54_RS15135 at 3342.293 kb on + strand, within LRK54_RS15135 at 3342.293 kb on + strand, within LRK54_RS15135 at 3342.293 kb on + strand, within LRK54_RS15135 at 3342.293 kb on + strand, within LRK54_RS15135 at 3342.293 kb on + strand, within LRK54_RS15135 at 3342.293 kb on + strand, within LRK54_RS15135 at 3342.293 kb on + strand, within LRK54_RS15135 at 3342.294 kb on - strand, within LRK54_RS15135 at 3342.294 kb on - strand, within LRK54_RS15135 at 3342.294 kb on - strand, within LRK54_RS15135 at 3342.294 kb on - strand, within LRK54_RS15135 at 3342.294 kb on - strand, within LRK54_RS15135 at 3342.294 kb on - strand, within LRK54_RS15135 at 3342.493 kb on + strand, within LRK54_RS15135 at 3342.493 kb on + strand, within LRK54_RS15135 at 3342.787 kb on + strand at 3342.787 kb on + strand at 3342.787 kb on + strand at 3342.788 kb on - strand at 3342.788 kb on - strand at 3342.788 kb on - strand at 3343.398 kb on + strand at 3343.398 kb on + strand at 3343.398 kb on + strand at 3343.398 kb on + strand at 3343.398 kb on + strand at 3343.398 kb on + strand at 3343.398 kb on + strand at 3343.399 kb on - strand at 3343.399 kb on - strand at 3343.399 kb on - strand at 3343.399 kb on - strand at 3343.399 kb on - strand at 3343.399 kb on - strand at 3343.399 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium bromide 250 mM remove 3,341,812 - LRK54_RS15135 0.34 +0.8 3,341,886 + LRK54_RS15135 0.39 -1.0 3,341,886 + LRK54_RS15135 0.39 -0.6 3,341,909 + LRK54_RS15135 0.40 -1.2 3,341,909 + LRK54_RS15135 0.40 +0.1 3,341,909 + LRK54_RS15135 0.40 +1.2 3,341,909 + LRK54_RS15135 0.40 -0.7 3,341,909 + LRK54_RS15135 0.40 -0.0 3,341,909 + LRK54_RS15135 0.40 +0.6 3,341,910 - LRK54_RS15135 0.40 -0.1 3,341,910 - LRK54_RS15135 0.40 +1.2 3,341,910 - LRK54_RS15135 0.40 +0.6 3,341,910 - LRK54_RS15135 0.40 +1.0 3,341,910 - LRK54_RS15135 0.40 -1.9 3,342,034 + LRK54_RS15135 0.49 +0.1 3,342,034 + LRK54_RS15135 0.49 +0.5 3,342,034 + LRK54_RS15135 0.49 +0.6 3,342,034 + LRK54_RS15135 0.49 -1.2 3,342,034 + LRK54_RS15135 0.49 +1.0 3,342,034 + LRK54_RS15135 0.49 +1.0 3,342,034 + LRK54_RS15135 0.49 +1.0 3,342,034 + LRK54_RS15135 0.49 -0.5 3,342,034 + LRK54_RS15135 0.49 +0.1 3,342,064 + LRK54_RS15135 0.51 +0.2 3,342,293 + LRK54_RS15135 0.66 +1.2 3,342,293 + LRK54_RS15135 0.66 +0.5 3,342,293 + LRK54_RS15135 0.66 -0.0 3,342,293 + LRK54_RS15135 0.66 +0.8 3,342,293 + LRK54_RS15135 0.66 +0.8 3,342,293 + LRK54_RS15135 0.66 -1.1 3,342,293 + LRK54_RS15135 0.66 +0.2 3,342,293 + LRK54_RS15135 0.66 +0.2 3,342,293 + LRK54_RS15135 0.66 -1.5 3,342,293 + LRK54_RS15135 0.66 +0.3 3,342,293 + LRK54_RS15135 0.66 -1.4 3,342,293 + LRK54_RS15135 0.66 +0.8 3,342,293 + LRK54_RS15135 0.66 +0.4 3,342,293 + LRK54_RS15135 0.66 +0.2 3,342,293 + LRK54_RS15135 0.66 -0.3 3,342,293 + LRK54_RS15135 0.66 -0.9 3,342,294 - LRK54_RS15135 0.66 -0.4 3,342,294 - LRK54_RS15135 0.66 -0.4 3,342,294 - LRK54_RS15135 0.66 -1.1 3,342,294 - LRK54_RS15135 0.66 +0.2 3,342,294 - LRK54_RS15135 0.66 +0.3 3,342,294 - LRK54_RS15135 0.66 -1.9 3,342,493 + LRK54_RS15135 0.79 +2.3 3,342,493 + LRK54_RS15135 0.79 -0.2 3,342,787 + -1.0 3,342,787 + +0.2 3,342,787 + +0.8 3,342,788 - +0.4 3,342,788 - +0.1 3,342,788 - +0.8 3,343,398 + -0.2 3,343,398 + -0.8 3,343,398 + -0.1 3,343,398 + -0.1 3,343,398 + -2.4 3,343,398 + -1.8 3,343,398 + -0.2 3,343,399 - +0.6 3,343,399 - -0.5 3,343,399 - -0.6 3,343,399 - -1.1 3,343,399 - -0.9 3,343,399 - +1.1 3,343,399 - +0.1
Or see this region's nucleotide sequence