Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2539 and Dshi_2540 are separated by 65 nucleotides Dshi_2540 and Dshi_2541 are separated by 154 nucleotides Dshi_2541 and Dshi_2542 are separated by 183 nucleotides
Dshi_2539: Dshi_2539 - GntR domain protein (RefSeq), at 2,696,193 to 2,696,882
_2539
Dshi_2540: Dshi_2540 - hypothetical protein (RefSeq), at 2,696,948 to 2,697,499
_2540
Dshi_2541: Dshi_2541 - GntR domain protein (RefSeq), at 2,697,654 to 2,698,334
_2541
Dshi_2542: Dshi_2542 - 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase (RefSeq), at 2,698,518 to 2,699,444
_2542
Position (kb)
2697
2698
2699 Strain fitness (log2 ratio)
-2
-1
0
1 at 2696.655 kb on + strand, within Dshi_2539 at 2696.686 kb on + strand, within Dshi_2539 at 2696.712 kb on + strand, within Dshi_2539 at 2696.720 kb on - strand, within Dshi_2539 at 2696.733 kb on - strand, within Dshi_2539 at 2696.757 kb on + strand, within Dshi_2539 at 2696.772 kb on - strand, within Dshi_2539 at 2696.772 kb on - strand, within Dshi_2539 at 2696.809 kb on + strand, within Dshi_2539 at 2696.843 kb on + strand at 2696.884 kb on - strand at 2696.889 kb on - strand at 2696.905 kb on - strand at 2696.924 kb on + strand at 2697.012 kb on + strand, within Dshi_2540 at 2697.038 kb on + strand, within Dshi_2540 at 2697.038 kb on + strand, within Dshi_2540 at 2697.049 kb on - strand, within Dshi_2540 at 2697.098 kb on - strand, within Dshi_2540 at 2697.113 kb on + strand, within Dshi_2540 at 2697.157 kb on - strand, within Dshi_2540 at 2697.282 kb on + strand, within Dshi_2540 at 2697.301 kb on - strand, within Dshi_2540 at 2697.384 kb on + strand, within Dshi_2540 at 2697.434 kb on + strand, within Dshi_2540 at 2697.434 kb on + strand, within Dshi_2540 at 2697.434 kb on + strand, within Dshi_2540 at 2697.442 kb on - strand, within Dshi_2540 at 2697.442 kb on - strand, within Dshi_2540 at 2697.459 kb on - strand at 2697.517 kb on + strand at 2697.524 kb on + strand at 2697.525 kb on - strand at 2697.543 kb on + strand at 2697.641 kb on - strand at 2697.649 kb on - strand at 2697.649 kb on - strand at 2697.652 kb on + strand at 2697.652 kb on + strand at 2697.669 kb on - strand at 2697.673 kb on + strand at 2697.725 kb on - strand, within Dshi_2541 at 2697.744 kb on + strand, within Dshi_2541 at 2697.821 kb on - strand, within Dshi_2541 at 2697.832 kb on - strand, within Dshi_2541 at 2697.835 kb on - strand, within Dshi_2541 at 2697.888 kb on + strand, within Dshi_2541 at 2697.935 kb on + strand, within Dshi_2541 at 2697.935 kb on + strand, within Dshi_2541 at 2697.971 kb on + strand, within Dshi_2541 at 2697.974 kb on - strand, within Dshi_2541 at 2698.027 kb on + strand, within Dshi_2541 at 2698.056 kb on - strand, within Dshi_2541 at 2698.063 kb on + strand, within Dshi_2541 at 2698.063 kb on + strand, within Dshi_2541 at 2698.071 kb on - strand, within Dshi_2541 at 2698.231 kb on + strand, within Dshi_2541 at 2698.279 kb on - strand at 2698.383 kb on - strand at 2698.407 kb on - strand at 2698.409 kb on + strand at 2698.442 kb on - strand at 2698.466 kb on - strand at 2698.508 kb on + strand at 2698.516 kb on + strand at 2698.558 kb on - strand at 2698.584 kb on + strand at 2698.584 kb on + strand at 2698.597 kb on - strand at 2698.643 kb on - strand, within Dshi_2542 at 2698.674 kb on + strand, within Dshi_2542 at 2698.774 kb on - strand, within Dshi_2542 at 2698.809 kb on + strand, within Dshi_2542 at 2698.809 kb on + strand, within Dshi_2542 at 2698.809 kb on + strand, within Dshi_2542 at 2698.809 kb on + strand, within Dshi_2542 at 2698.809 kb on + strand at 2698.817 kb on - strand, within Dshi_2542 at 2698.829 kb on - strand, within Dshi_2542 at 2698.855 kb on - strand, within Dshi_2542 at 2698.932 kb on + strand, within Dshi_2542 at 2698.980 kb on - strand, within Dshi_2542 at 2699.004 kb on - strand, within Dshi_2542 at 2699.014 kb on + strand, within Dshi_2542 at 2699.045 kb on + strand, within Dshi_2542 at 2699.088 kb on - strand, within Dshi_2542 at 2699.131 kb on + strand, within Dshi_2542 at 2699.131 kb on + strand, within Dshi_2542 at 2699.131 kb on + strand, within Dshi_2542 at 2699.131 kb on + strand, within Dshi_2542 at 2699.139 kb on - strand, within Dshi_2542 at 2699.139 kb on - strand, within Dshi_2542 at 2699.139 kb on - strand, within Dshi_2542 at 2699.290 kb on - strand, within Dshi_2542 at 2699.296 kb on + strand, within Dshi_2542
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 2,696,655 + Dshi_2539 0.67 -0.8 2,696,686 + Dshi_2539 0.71 -0.4 2,696,712 + Dshi_2539 0.75 +0.7 2,696,720 - Dshi_2539 0.76 -0.7 2,696,733 - Dshi_2539 0.78 +0.3 2,696,757 + Dshi_2539 0.82 -1.0 2,696,772 - Dshi_2539 0.84 -0.3 2,696,772 - Dshi_2539 0.84 -0.7 2,696,809 + Dshi_2539 0.89 -0.7 2,696,843 + -0.3 2,696,884 - -0.5 2,696,889 - -0.0 2,696,905 - -0.3 2,696,924 + +0.8 2,697,012 + Dshi_2540 0.12 +0.4 2,697,038 + Dshi_2540 0.16 -1.3 2,697,038 + Dshi_2540 0.16 -0.3 2,697,049 - Dshi_2540 0.18 +1.0 2,697,098 - Dshi_2540 0.27 -0.1 2,697,113 + Dshi_2540 0.30 -0.6 2,697,157 - Dshi_2540 0.38 -0.6 2,697,282 + Dshi_2540 0.61 -0.2 2,697,301 - Dshi_2540 0.64 +0.3 2,697,384 + Dshi_2540 0.79 -0.7 2,697,434 + Dshi_2540 0.88 +0.5 2,697,434 + Dshi_2540 0.88 -0.5 2,697,434 + Dshi_2540 0.88 -1.9 2,697,442 - Dshi_2540 0.89 -1.2 2,697,442 - Dshi_2540 0.89 -1.7 2,697,459 - -0.5 2,697,517 + -0.2 2,697,524 + -0.7 2,697,525 - +0.4 2,697,543 + -0.2 2,697,641 - -1.3 2,697,649 - -0.6 2,697,649 - -0.4 2,697,652 + +0.2 2,697,652 + -0.6 2,697,669 - -0.4 2,697,673 + -0.5 2,697,725 - Dshi_2541 0.10 -0.6 2,697,744 + Dshi_2541 0.13 +0.3 2,697,821 - Dshi_2541 0.25 -0.3 2,697,832 - Dshi_2541 0.26 +0.1 2,697,835 - Dshi_2541 0.27 -0.7 2,697,888 + Dshi_2541 0.34 -0.5 2,697,935 + Dshi_2541 0.41 +0.2 2,697,935 + Dshi_2541 0.41 -0.4 2,697,971 + Dshi_2541 0.47 -1.7 2,697,974 - Dshi_2541 0.47 -0.5 2,698,027 + Dshi_2541 0.55 -1.0 2,698,056 - Dshi_2541 0.59 -2.1 2,698,063 + Dshi_2541 0.60 -0.2 2,698,063 + Dshi_2541 0.60 -1.0 2,698,071 - Dshi_2541 0.61 -0.9 2,698,231 + Dshi_2541 0.85 -2.3 2,698,279 - -0.1 2,698,383 - +0.1 2,698,407 - +0.1 2,698,409 + +0.3 2,698,442 - -0.9 2,698,466 - -0.3 2,698,508 + +0.4 2,698,516 + +0.1 2,698,558 - -0.8 2,698,584 + -0.4 2,698,584 + -0.4 2,698,597 - -0.3 2,698,643 - Dshi_2542 0.13 -0.3 2,698,674 + Dshi_2542 0.17 -0.5 2,698,774 - Dshi_2542 0.28 -0.1 2,698,809 + Dshi_2542 0.31 -2.2 2,698,809 + Dshi_2542 0.31 +0.3 2,698,809 + Dshi_2542 0.31 -0.4 2,698,809 + Dshi_2542 0.31 +0.0 2,698,809 + -0.3 2,698,817 - Dshi_2542 0.32 -1.1 2,698,829 - Dshi_2542 0.34 +0.6 2,698,855 - Dshi_2542 0.36 -0.1 2,698,932 + Dshi_2542 0.45 -0.5 2,698,980 - Dshi_2542 0.50 -1.0 2,699,004 - Dshi_2542 0.52 -0.4 2,699,014 + Dshi_2542 0.54 -0.6 2,699,045 + Dshi_2542 0.57 -1.6 2,699,088 - Dshi_2542 0.61 -0.0 2,699,131 + Dshi_2542 0.66 +0.6 2,699,131 + Dshi_2542 0.66 +0.2 2,699,131 + Dshi_2542 0.66 -0.7 2,699,131 + Dshi_2542 0.66 -0.2 2,699,139 - Dshi_2542 0.67 -0.0 2,699,139 - Dshi_2542 0.67 +0.7 2,699,139 - Dshi_2542 0.67 +0.0 2,699,290 - Dshi_2542 0.83 +0.6 2,699,296 + Dshi_2542 0.84 -0.6
Or see this region's nucleotide sequence