Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_R0043 and Dshi_2313 are separated by 60 nucleotides Dshi_2313 and Dshi_2314 overlap by 4 nucleotides Dshi_2314 and Dshi_2315 are separated by 52 nucleotides
Dshi_R0043: Dshi_R0043 - tRNA-Ser (RefSeq), at 2,444,564 to 2,444,653
_R0043
Dshi_2313: Dshi_2313 - Threonyl/alanyl tRNA synthetase SAD (RefSeq), at 2,444,714 to 2,445,433
_2313
Dshi_2314: Dshi_2314 - Pyridoxal-5'-phosphate-dependent protein beta subunit (RefSeq), at 2,445,430 to 2,446,467
_2314
Dshi_2315: Dshi_2315 - NUDIX hydrolase (RefSeq), at 2,446,520 to 2,447,656
_2315
Position (kb)
2445
2446
2447 Strain fitness (log2 ratio)
-1
0
1 at 2444.435 kb on - strand at 2444.446 kb on - strand at 2444.495 kb on - strand at 2444.500 kb on - strand at 2444.501 kb on - strand at 2444.700 kb on + strand at 2444.797 kb on - strand, within Dshi_2313 at 2444.812 kb on - strand, within Dshi_2313 at 2444.828 kb on - strand, within Dshi_2313 at 2444.887 kb on - strand, within Dshi_2313 at 2444.910 kb on + strand, within Dshi_2313 at 2444.918 kb on - strand, within Dshi_2313 at 2444.918 kb on - strand, within Dshi_2313 at 2445.031 kb on + strand, within Dshi_2313 at 2445.124 kb on - strand, within Dshi_2313 at 2445.124 kb on - strand, within Dshi_2313 at 2445.161 kb on + strand, within Dshi_2313 at 2445.180 kb on - strand, within Dshi_2313 at 2445.209 kb on - strand, within Dshi_2313 at 2445.442 kb on + strand at 2445.482 kb on - strand at 2446.477 kb on + strand at 2446.544 kb on + strand at 2446.552 kb on - strand at 2446.558 kb on + strand at 2446.731 kb on - strand, within Dshi_2315 at 2446.731 kb on - strand, within Dshi_2315 at 2446.731 kb on - strand, within Dshi_2315 at 2446.761 kb on + strand, within Dshi_2315 at 2446.908 kb on - strand, within Dshi_2315 at 2447.010 kb on + strand, within Dshi_2315 at 2447.122 kb on + strand, within Dshi_2315 at 2447.159 kb on - strand, within Dshi_2315 at 2447.307 kb on - strand, within Dshi_2315 at 2447.323 kb on - strand, within Dshi_2315 at 2447.333 kb on - strand, within Dshi_2315
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 2,444,435 - +0.1 2,444,446 - +0.1 2,444,495 - +0.0 2,444,500 - -0.2 2,444,501 - +0.5 2,444,700 + +0.7 2,444,797 - Dshi_2313 0.12 +0.1 2,444,812 - Dshi_2313 0.14 +0.7 2,444,828 - Dshi_2313 0.16 -1.1 2,444,887 - Dshi_2313 0.24 -0.2 2,444,910 + Dshi_2313 0.27 -0.7 2,444,918 - Dshi_2313 0.28 +0.1 2,444,918 - Dshi_2313 0.28 -0.1 2,445,031 + Dshi_2313 0.44 +0.7 2,445,124 - Dshi_2313 0.57 +0.0 2,445,124 - Dshi_2313 0.57 -1.5 2,445,161 + Dshi_2313 0.62 -1.4 2,445,180 - Dshi_2313 0.65 +0.1 2,445,209 - Dshi_2313 0.69 +0.0 2,445,442 + +1.0 2,445,482 - +0.6 2,446,477 + +0.4 2,446,544 + -0.9 2,446,552 - +0.9 2,446,558 + +0.4 2,446,731 - Dshi_2315 0.19 +0.5 2,446,731 - Dshi_2315 0.19 -0.7 2,446,731 - Dshi_2315 0.19 -0.8 2,446,761 + Dshi_2315 0.21 -0.8 2,446,908 - Dshi_2315 0.34 +0.4 2,447,010 + Dshi_2315 0.43 -0.4 2,447,122 + Dshi_2315 0.53 -0.5 2,447,159 - Dshi_2315 0.56 -0.5 2,447,307 - Dshi_2315 0.69 +0.8 2,447,323 - Dshi_2315 0.71 -0.5 2,447,333 - Dshi_2315 0.72 -0.2
Or see this region's nucleotide sequence