Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1017 and Dshi_1018 are separated by 59 nucleotides Dshi_1018 and Dshi_1019 are separated by 11 nucleotides Dshi_1019 and Dshi_1020 are separated by 136 nucleotides
Dshi_1017: Dshi_1017 - TonB-dependent heme/hemoglobin receptor family protein (RefSeq), at 1,051,254 to 1,053,368
_1017
Dshi_1018: Dshi_1018 - hypothetical protein (RefSeq), at 1,053,428 to 1,053,700
_1018
Dshi_1019: Dshi_1019 - RNA modification enzyme, MiaB family (RefSeq), at 1,053,712 to 1,055,025
_1019
Dshi_1020: Dshi_1020 - hypothetical protein (RefSeq), at 1,055,162 to 1,056,058
_1020
Position (kb)
1053
1054
1055
1056 Strain fitness (log2 ratio)
-2
-1
0
1 at 1052.716 kb on - strand, within Dshi_1017 at 1052.742 kb on + strand, within Dshi_1017 at 1052.746 kb on + strand, within Dshi_1017 at 1052.810 kb on + strand, within Dshi_1017 at 1052.828 kb on - strand, within Dshi_1017 at 1052.867 kb on - strand, within Dshi_1017 at 1052.880 kb on + strand, within Dshi_1017 at 1052.880 kb on + strand, within Dshi_1017 at 1052.888 kb on - strand, within Dshi_1017 at 1052.911 kb on - strand, within Dshi_1017 at 1052.943 kb on + strand, within Dshi_1017 at 1053.007 kb on + strand, within Dshi_1017 at 1053.007 kb on - strand, within Dshi_1017 at 1053.015 kb on - strand, within Dshi_1017 at 1053.046 kb on + strand, within Dshi_1017 at 1053.091 kb on + strand, within Dshi_1017 at 1053.091 kb on + strand, within Dshi_1017 at 1053.177 kb on + strand at 1053.220 kb on - strand at 1053.248 kb on + strand at 1053.304 kb on + strand at 1053.312 kb on - strand at 1053.312 kb on - strand at 1053.353 kb on + strand at 1053.405 kb on - strand at 1053.545 kb on + strand, within Dshi_1018 at 1053.927 kb on - strand, within Dshi_1019 at 1054.043 kb on + strand, within Dshi_1019 at 1054.048 kb on + strand, within Dshi_1019 at 1054.055 kb on + strand, within Dshi_1019 at 1054.055 kb on + strand, within Dshi_1019 at 1054.055 kb on + strand, within Dshi_1019 at 1054.063 kb on - strand, within Dshi_1019 at 1054.065 kb on + strand, within Dshi_1019 at 1054.073 kb on - strand, within Dshi_1019 at 1054.092 kb on - strand, within Dshi_1019 at 1054.226 kb on + strand, within Dshi_1019 at 1054.448 kb on + strand, within Dshi_1019 at 1054.456 kb on - strand, within Dshi_1019 at 1054.472 kb on + strand, within Dshi_1019 at 1054.614 kb on + strand, within Dshi_1019 at 1054.664 kb on + strand, within Dshi_1019 at 1054.757 kb on + strand, within Dshi_1019 at 1054.757 kb on + strand, within Dshi_1019 at 1054.829 kb on - strand, within Dshi_1019 at 1054.836 kb on + strand, within Dshi_1019 at 1054.844 kb on - strand, within Dshi_1019 at 1054.872 kb on + strand, within Dshi_1019 at 1054.888 kb on - strand, within Dshi_1019 at 1054.893 kb on - strand, within Dshi_1019 at 1054.934 kb on - strand at 1054.945 kb on + strand at 1054.946 kb on + strand at 1054.948 kb on - strand at 1054.952 kb on + strand at 1054.952 kb on + strand at 1054.953 kb on - strand at 1054.966 kb on + strand at 1055.000 kb on + strand at 1055.047 kb on + strand at 1055.049 kb on - strand at 1055.052 kb on + strand at 1055.079 kb on + strand at 1055.080 kb on - strand at 1055.096 kb on + strand at 1055.096 kb on + strand at 1055.104 kb on + strand at 1055.112 kb on - strand at 1055.123 kb on + strand at 1055.170 kb on + strand at 1055.170 kb on + strand at 1055.170 kb on + strand at 1055.173 kb on - strand at 1055.178 kb on - strand at 1055.198 kb on - strand at 1055.242 kb on + strand at 1055.278 kb on + strand, within Dshi_1020 at 1055.283 kb on + strand, within Dshi_1020 at 1055.283 kb on + strand, within Dshi_1020 at 1055.302 kb on - strand, within Dshi_1020 at 1055.302 kb on - strand, within Dshi_1020 at 1055.384 kb on - strand, within Dshi_1020 at 1055.413 kb on + strand, within Dshi_1020 at 1055.484 kb on - strand, within Dshi_1020 at 1055.514 kb on - strand, within Dshi_1020 at 1055.514 kb on - strand, within Dshi_1020 at 1055.537 kb on + strand, within Dshi_1020 at 1055.564 kb on - strand, within Dshi_1020 at 1055.567 kb on - strand, within Dshi_1020 at 1055.574 kb on + strand, within Dshi_1020 at 1055.584 kb on + strand, within Dshi_1020 at 1055.587 kb on - strand, within Dshi_1020 at 1055.594 kb on - strand, within Dshi_1020 at 1055.620 kb on - strand, within Dshi_1020 at 1055.628 kb on + strand, within Dshi_1020 at 1055.642 kb on + strand, within Dshi_1020 at 1055.683 kb on + strand, within Dshi_1020 at 1055.688 kb on + strand, within Dshi_1020 at 1055.707 kb on - strand, within Dshi_1020 at 1055.798 kb on + strand, within Dshi_1020 at 1055.806 kb on - strand, within Dshi_1020 at 1055.811 kb on - strand, within Dshi_1020 at 1055.827 kb on - strand, within Dshi_1020 at 1055.858 kb on + strand, within Dshi_1020 at 1055.890 kb on - strand, within Dshi_1020 at 1055.894 kb on - strand, within Dshi_1020 at 1055.960 kb on - strand, within Dshi_1020 at 1056.022 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 1,052,716 - Dshi_1017 0.69 -0.9 1,052,742 + Dshi_1017 0.70 -0.0 1,052,746 + Dshi_1017 0.71 +0.1 1,052,810 + Dshi_1017 0.74 +0.2 1,052,828 - Dshi_1017 0.74 -0.8 1,052,867 - Dshi_1017 0.76 -0.6 1,052,880 + Dshi_1017 0.77 -0.5 1,052,880 + Dshi_1017 0.77 +0.3 1,052,888 - Dshi_1017 0.77 -0.0 1,052,911 - Dshi_1017 0.78 +1.0 1,052,943 + Dshi_1017 0.80 -0.3 1,053,007 + Dshi_1017 0.83 -0.1 1,053,007 - Dshi_1017 0.83 -0.9 1,053,015 - Dshi_1017 0.83 -0.5 1,053,046 + Dshi_1017 0.85 -0.5 1,053,091 + Dshi_1017 0.87 -1.3 1,053,091 + Dshi_1017 0.87 -1.6 1,053,177 + +0.2 1,053,220 - +0.1 1,053,248 + -1.0 1,053,304 + -0.1 1,053,312 - +0.4 1,053,312 - -0.8 1,053,353 + -0.9 1,053,405 - +0.2 1,053,545 + Dshi_1018 0.43 +0.1 1,053,927 - Dshi_1019 0.16 -2.2 1,054,043 + Dshi_1019 0.25 -0.9 1,054,048 + Dshi_1019 0.26 -0.5 1,054,055 + Dshi_1019 0.26 -0.1 1,054,055 + Dshi_1019 0.26 +0.3 1,054,055 + Dshi_1019 0.26 -0.6 1,054,063 - Dshi_1019 0.27 -1.0 1,054,065 + Dshi_1019 0.27 -2.6 1,054,073 - Dshi_1019 0.27 +1.2 1,054,092 - Dshi_1019 0.29 -1.8 1,054,226 + Dshi_1019 0.39 +0.3 1,054,448 + Dshi_1019 0.56 -1.2 1,054,456 - Dshi_1019 0.57 -1.7 1,054,472 + Dshi_1019 0.58 -0.4 1,054,614 + Dshi_1019 0.69 -0.6 1,054,664 + Dshi_1019 0.72 -0.7 1,054,757 + Dshi_1019 0.80 -0.8 1,054,757 + Dshi_1019 0.80 -0.6 1,054,829 - Dshi_1019 0.85 -0.9 1,054,836 + Dshi_1019 0.86 -1.3 1,054,844 - Dshi_1019 0.86 -0.8 1,054,872 + Dshi_1019 0.88 -0.5 1,054,888 - Dshi_1019 0.89 -1.3 1,054,893 - Dshi_1019 0.90 -0.7 1,054,934 - -1.0 1,054,945 + -0.5 1,054,946 + +0.8 1,054,948 - +0.0 1,054,952 + +0.2 1,054,952 + -0.3 1,054,953 - +0.2 1,054,966 + +0.2 1,055,000 + +0.0 1,055,047 + -1.1 1,055,049 - -1.0 1,055,052 + -1.4 1,055,079 + -0.7 1,055,080 - -1.4 1,055,096 + -1.9 1,055,096 + +0.2 1,055,104 + +0.9 1,055,112 - -0.5 1,055,123 + +0.2 1,055,170 + -0.3 1,055,170 + -0.7 1,055,170 + -0.6 1,055,173 - -1.0 1,055,178 - -0.7 1,055,198 - -0.0 1,055,242 + -0.2 1,055,278 + Dshi_1020 0.13 +0.1 1,055,283 + Dshi_1020 0.13 +0.1 1,055,283 + Dshi_1020 0.13 -0.3 1,055,302 - Dshi_1020 0.16 -1.2 1,055,302 - Dshi_1020 0.16 -0.4 1,055,384 - Dshi_1020 0.25 -0.3 1,055,413 + Dshi_1020 0.28 +0.1 1,055,484 - Dshi_1020 0.36 -0.0 1,055,514 - Dshi_1020 0.39 -1.2 1,055,514 - Dshi_1020 0.39 -2.1 1,055,537 + Dshi_1020 0.42 -1.2 1,055,564 - Dshi_1020 0.45 +0.2 1,055,567 - Dshi_1020 0.45 +0.2 1,055,574 + Dshi_1020 0.46 -0.1 1,055,584 + Dshi_1020 0.47 -1.6 1,055,587 - Dshi_1020 0.47 -0.8 1,055,594 - Dshi_1020 0.48 -0.0 1,055,620 - Dshi_1020 0.51 +0.2 1,055,628 + Dshi_1020 0.52 +0.3 1,055,642 + Dshi_1020 0.54 -0.2 1,055,683 + Dshi_1020 0.58 -0.1 1,055,688 + Dshi_1020 0.59 -0.4 1,055,707 - Dshi_1020 0.61 -0.2 1,055,798 + Dshi_1020 0.71 -1.0 1,055,806 - Dshi_1020 0.72 -0.6 1,055,811 - Dshi_1020 0.72 -0.1 1,055,827 - Dshi_1020 0.74 -0.5 1,055,858 + Dshi_1020 0.78 +0.1 1,055,890 - Dshi_1020 0.81 -0.2 1,055,894 - Dshi_1020 0.82 -0.1 1,055,960 - Dshi_1020 0.89 -0.4 1,056,022 - +0.6
Or see this region's nucleotide sequence