Experiment: Starch from potato (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3226 and BT3227 are separated by 65 nucleotides BT3227 and BT3228 overlap by 4 nucleotides BT3228 and BT3229 are separated by 28 nucleotides BT3229 and BT3230 are separated by 84 nucleotides
BT3226: BT3226 - hypothetical protein (NCBI ptt file), at 4,117,392 to 4,117,883
BT3226
BT3227: BT3227 - putative ribonuclease P protein component (NCBI ptt file), at 4,117,949 to 4,118,278
BT3227
BT3228: BT3228 - conserved hypothetical protein (NCBI ptt file), at 4,118,275 to 4,118,550
BT3228
BT3229: BT3229 - TatD-related DNase (NCBI ptt file), at 4,118,579 to 4,119,268
BT3229
BT3230: BT3230 - tyrosyl-tRNA synthetase (NCBI ptt file), at 4,119,353 to 4,120,645
BT3230
Position (kb)
4118
4119
4120 Strain fitness (log2 ratio)
-2
-1
0
1 at 4118.268 kb on + strand at 4118.351 kb on + strand, within BT3228 at 4118.483 kb on - strand, within BT3228 at 4118.559 kb on + strand at 4118.565 kb on - strand at 4118.579 kb on - strand at 4118.579 kb on - strand at 4118.582 kb on - strand at 4118.599 kb on + strand at 4118.600 kb on - strand at 4118.614 kb on + strand at 4118.615 kb on - strand at 4118.730 kb on - strand, within BT3229 at 4118.887 kb on - strand, within BT3229 at 4118.942 kb on - strand, within BT3229 at 4119.072 kb on - strand, within BT3229 at 4119.241 kb on + strand at 4119.242 kb on - strand at 4119.275 kb on + strand at 4119.326 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Starch from potato (C) remove 4,118,268 + -0.5 4,118,351 + BT3228 0.28 -0.4 4,118,483 - BT3228 0.75 +1.0 4,118,559 + +1.6 4,118,565 - -0.1 4,118,579 - +1.2 4,118,579 - +0.5 4,118,582 - +1.2 4,118,599 + -1.2 4,118,600 - +0.6 4,118,614 + -1.1 4,118,615 - -1.5 4,118,730 - BT3229 0.22 -1.8 4,118,887 - BT3229 0.45 -2.5 4,118,942 - BT3229 0.53 -0.7 4,119,072 - BT3229 0.71 -0.1 4,119,241 + -0.3 4,119,242 - -1.3 4,119,275 + -1.6 4,119,326 + +0.8
Or see this region's nucleotide sequence