Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt queF and Shew_2655 are separated by 322 nucleotides Shew_2655 and Shew_2656 are separated by 201 nucleotides
Shew_2654: queF - 7-cyano-7-deazaguanine reductase (RefSeq), at 3,127,090 to 3,127,980
queF
Shew_2655: Shew_2655 - hypothetical protein (RefSeq), at 3,128,303 to 3,129,493
_2655
Shew_2656: Shew_2656 - 4'-phosphopantetheinyl transferase (RefSeq), at 3,129,695 to 3,130,606
_2656
Position (kb)
3128
3129
3130 Strain fitness (log2 ratio)
-1
0
1 at 3127.427 kb on + strand, within queF at 3127.676 kb on + strand, within queF at 3127.676 kb on + strand, within queF at 3127.684 kb on - strand, within queF at 3128.051 kb on + strand at 3128.059 kb on - strand at 3128.059 kb on - strand at 3128.069 kb on - strand at 3128.071 kb on - strand at 3128.442 kb on + strand, within Shew_2655 at 3128.500 kb on - strand, within Shew_2655 at 3128.636 kb on + strand, within Shew_2655 at 3128.643 kb on - strand, within Shew_2655 at 3128.752 kb on - strand, within Shew_2655 at 3128.754 kb on - strand, within Shew_2655 at 3128.960 kb on - strand, within Shew_2655 at 3129.179 kb on + strand, within Shew_2655 at 3129.690 kb on + strand at 3129.697 kb on - strand at 3130.038 kb on + strand, within Shew_2656 at 3130.274 kb on - strand, within Shew_2656 at 3130.281 kb on + strand, within Shew_2656 at 3130.289 kb on - strand, within Shew_2656 at 3130.329 kb on - strand, within Shew_2656 at 3130.429 kb on + strand, within Shew_2656
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,127,427 + queF Shew_2654 0.38 +0.0 3,127,676 + queF Shew_2654 0.66 +0.3 3,127,676 + queF Shew_2654 0.66 +1.3 3,127,684 - queF Shew_2654 0.67 +0.5 3,128,051 + +0.6 3,128,059 - -0.1 3,128,059 - +0.2 3,128,069 - -0.0 3,128,071 - -0.1 3,128,442 + Shew_2655 0.12 +0.2 3,128,500 - Shew_2655 0.17 -0.0 3,128,636 + Shew_2655 0.28 +1.0 3,128,643 - Shew_2655 0.29 +0.2 3,128,752 - Shew_2655 0.38 +0.1 3,128,754 - Shew_2655 0.38 +1.3 3,128,960 - Shew_2655 0.55 +0.1 3,129,179 + Shew_2655 0.74 +0.1 3,129,690 + -0.1 3,129,697 - +0.0 3,130,038 + Shew_2656 0.38 +0.4 3,130,274 - Shew_2656 0.63 +0.6 3,130,281 + Shew_2656 0.64 +0.1 3,130,289 - Shew_2656 0.65 +0.3 3,130,329 - Shew_2656 0.70 +0.9 3,130,429 + Shew_2656 0.80 +0.2
Or see this region's nucleotide sequence