Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_4111 and Dshi_4112 are separated by 636 nucleotides Dshi_4112 and Dshi_4113 overlap by 4 nucleotides Dshi_4113 and Dshi_4114 are separated by 186 nucleotides Dshi_4114 and Dshi_4115 are separated by 4 nucleotides
Dshi_4111: Dshi_4111 - integrase family protein (RefSeq), at 41,989 to 44,979
_4111
Dshi_4112: Dshi_4112 - prevent-host-death family protein (RefSeq), at 45,616 to 45,843
_4112
Dshi_4113: Dshi_4113 - death-on-curing family protein (RefSeq), at 45,840 to 46,232
_4113
Dshi_4114: Dshi_4114 - dTDP-4-dehydrorhamnose 3,5-epimerase (RefSeq), at 46,419 to 46,973
_4114
Dshi_4115: Dshi_4115 - dTDP-glucose 4,6-dehydratase (RefSeq), at 46,978 to 48,018
_4115
Position (kb) 45
46
47 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 44.843 kb on + strand at 44.843 kb on - strand at 44.858 kb on + strand at 44.858 kb on + strand at 44.858 kb on + strand at 44.858 kb on + strand at 44.858 kb on + strand at 44.858 kb on + strand at 44.866 kb on - strand at 44.876 kb on - strand at 44.885 kb on - strand at 44.920 kb on + strand at 44.921 kb on - strand at 44.923 kb on - strand at 44.928 kb on - strand at 44.929 kb on - strand at 44.954 kb on + strand at 44.956 kb on - strand at 44.973 kb on + strand at 44.973 kb on + strand at 44.981 kb on - strand at 44.981 kb on - strand at 44.981 kb on - strand at 44.981 kb on - strand at 45.000 kb on + strand at 45.024 kb on + strand at 45.037 kb on + strand at 45.037 kb on + strand at 45.037 kb on + strand at 45.037 kb on + strand at 45.045 kb on - strand at 45.045 kb on - strand at 45.045 kb on - strand at 45.100 kb on + strand at 45.108 kb on - strand at 45.108 kb on - strand at 45.108 kb on - strand at 45.143 kb on + strand at 45.144 kb on + strand at 45.144 kb on + strand at 45.158 kb on - strand at 45.160 kb on + strand at 45.168 kb on - strand at 45.180 kb on + strand at 45.199 kb on - strand at 45.208 kb on - strand at 45.221 kb on - strand at 45.221 kb on - strand at 45.233 kb on - strand at 45.262 kb on + strand at 45.276 kb on + strand at 45.312 kb on - strand at 45.312 kb on - strand at 45.313 kb on + strand at 45.317 kb on + strand at 45.317 kb on + strand at 45.317 kb on - strand at 45.327 kb on + strand at 45.340 kb on - strand at 45.372 kb on + strand at 45.384 kb on + strand at 45.407 kb on - strand at 45.408 kb on - strand at 45.410 kb on + strand at 45.422 kb on - strand at 45.423 kb on + strand at 45.423 kb on - strand at 45.423 kb on - strand at 45.423 kb on - strand at 45.432 kb on - strand at 45.438 kb on - strand at 45.446 kb on + strand at 45.454 kb on - strand at 45.503 kb on + strand at 45.506 kb on - strand at 45.508 kb on + strand at 45.508 kb on + strand at 45.536 kb on + strand at 45.547 kb on + strand at 45.547 kb on + strand at 45.555 kb on - strand at 45.555 kb on - strand at 45.944 kb on - strand, within Dshi_4113 at 45.951 kb on + strand, within Dshi_4113 at 46.229 kb on - strand at 46.435 kb on + strand at 46.528 kb on + strand, within Dshi_4114 at 46.590 kb on + strand, within Dshi_4114 at 47.071 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 44,843 + -0.2 44,843 - -0.7 44,858 + -0.9 44,858 + +1.0 44,858 + -0.5 44,858 + -0.2 44,858 + -1.9 44,858 + -0.5 44,866 - +0.1 44,876 - -0.5 44,885 - +0.1 44,920 + +0.3 44,921 - -0.1 44,923 - +0.1 44,928 - -0.5 44,929 - -0.3 44,954 + +1.2 44,956 - -0.8 44,973 + -0.0 44,973 + -0.1 44,981 - -0.3 44,981 - -1.3 44,981 - -1.5 44,981 - -1.1 45,000 + +0.5 45,024 + -1.3 45,037 + -1.1 45,037 + -0.4 45,037 + +1.4 45,037 + -2.2 45,045 - -0.5 45,045 - -0.7 45,045 - +1.1 45,100 + -0.2 45,108 - -0.5 45,108 - -0.2 45,108 - +0.4 45,143 + -0.8 45,144 + -0.4 45,144 + -0.2 45,158 - +0.1 45,160 + -0.7 45,168 - +0.5 45,180 + +0.1 45,199 - -0.4 45,208 - -0.6 45,221 - -0.8 45,221 - -0.4 45,233 - -0.5 45,262 + +0.5 45,276 + +0.8 45,312 - -1.1 45,312 - +1.3 45,313 + -0.2 45,317 + -0.1 45,317 + -0.1 45,317 - +1.6 45,327 + -0.2 45,340 - +0.2 45,372 + -1.1 45,384 + +1.5 45,407 - +0.6 45,408 - +0.1 45,410 + +0.3 45,422 - -0.3 45,423 + -2.9 45,423 - -0.2 45,423 - -0.2 45,423 - +0.2 45,432 - -0.3 45,438 - -0.9 45,446 + +0.6 45,454 - +1.8 45,503 + +0.7 45,506 - -0.1 45,508 + -2.5 45,508 + +2.0 45,536 + -1.0 45,547 + -0.9 45,547 + +1.1 45,555 - +0.7 45,555 - -0.3 45,944 - Dshi_4113 0.26 -0.5 45,951 + Dshi_4113 0.28 -0.5 46,229 - -1.5 46,435 + +0.1 46,528 + Dshi_4114 0.20 -0.9 46,590 + Dshi_4114 0.31 +0.2 47,071 + -0.2
Or see this region's nucleotide sequence