Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3091 and Dshi_3092 overlap by 4 nucleotides Dshi_3092 and Dshi_3093 overlap by 4 nucleotides Dshi_3093 and Dshi_3094 overlap by 1 nucleotides Dshi_3094 and Dshi_3095 overlap by 1 nucleotides Dshi_3095 and Dshi_3096 are separated by 2 nucleotides
Dshi_3091: Dshi_3091 - aspartate carbamoyltransferase (RefSeq), at 3,260,134 to 3,261,084
_3091
Dshi_3092: Dshi_3092 - hypothetical protein (RefSeq), at 3,261,081 to 3,261,506
_3092
Dshi_3093: Dshi_3093 - hypothetical protein (RefSeq), at 3,261,503 to 3,262,081
_3093
Dshi_3094: Dshi_3094 - dihydroorotase, multifunctional complex type (RefSeq), at 3,262,081 to 3,263,379
_3094
Dshi_3095: Dshi_3095 - protein of unknown function DUF205 (RefSeq), at 3,263,379 to 3,263,990
_3095
Dshi_3096: Dshi_3096 - protein of unknown function DUF805 (RefSeq), at 3,263,993 to 3,264,499
_3096
Position (kb)
3262
3263
3264 Strain fitness (log2 ratio)
-2
-1
0
1 at 3261.093 kb on - strand at 3261.237 kb on - strand, within Dshi_3092 at 3261.285 kb on - strand, within Dshi_3092 at 3261.359 kb on + strand, within Dshi_3092 at 3261.422 kb on + strand, within Dshi_3092 at 3261.589 kb on - strand, within Dshi_3093 at 3262.039 kb on - strand at 3262.209 kb on + strand at 3262.440 kb on + strand, within Dshi_3094 at 3262.527 kb on + strand, within Dshi_3094 at 3263.344 kb on - strand at 3263.998 kb on - strand at 3264.004 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 3,261,093 - -0.8 3,261,237 - Dshi_3092 0.37 +0.6 3,261,285 - Dshi_3092 0.48 -0.3 3,261,359 + Dshi_3092 0.65 -0.5 3,261,422 + Dshi_3092 0.80 -1.1 3,261,589 - Dshi_3093 0.15 -0.3 3,262,039 - +0.4 3,262,209 + -2.1 3,262,440 + Dshi_3094 0.28 -2.5 3,262,527 + Dshi_3094 0.34 -1.0 3,263,344 - -1.8 3,263,998 - +0.3 3,264,004 - +1.0
Or see this region's nucleotide sequence