Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3089 and Dshi_3090 overlap by 4 nucleotides Dshi_3090 and Dshi_3091 are separated by 109 nucleotides Dshi_3091 and Dshi_3092 overlap by 4 nucleotides Dshi_3092 and Dshi_3093 overlap by 4 nucleotides Dshi_3093 and Dshi_3094 overlap by 1 nucleotides
Dshi_3089: Dshi_3089 - molybdenum cofactor biosynthesis protein B (RefSeq), at 3,258,688 to 3,259,230
_3089
Dshi_3090: Dshi_3090 - phage SPO1 DNA polymerase-related protein (RefSeq), at 3,259,227 to 3,260,024
_3090
Dshi_3091: Dshi_3091 - aspartate carbamoyltransferase (RefSeq), at 3,260,134 to 3,261,084
_3091
Dshi_3092: Dshi_3092 - hypothetical protein (RefSeq), at 3,261,081 to 3,261,506
_3092
Dshi_3093: Dshi_3093 - hypothetical protein (RefSeq), at 3,261,503 to 3,262,081
_3093
Dshi_3094: Dshi_3094 - dihydroorotase, multifunctional complex type (RefSeq), at 3,262,081 to 3,263,379
_3094
Position (kb)
3260
3261
3262 Strain fitness (log2 ratio)
-2
-1
0
1 at 3259.212 kb on - strand at 3260.036 kb on + strand at 3260.115 kb on + strand at 3260.115 kb on + strand at 3260.128 kb on - strand at 3260.267 kb on + strand, within Dshi_3091 at 3260.267 kb on + strand, within Dshi_3091 at 3260.353 kb on - strand, within Dshi_3091 at 3260.398 kb on + strand, within Dshi_3091 at 3260.659 kb on - strand, within Dshi_3091 at 3260.708 kb on + strand, within Dshi_3091 at 3260.746 kb on + strand, within Dshi_3091 at 3260.757 kb on + strand, within Dshi_3091 at 3260.772 kb on + strand, within Dshi_3091 at 3261.093 kb on - strand at 3261.237 kb on - strand, within Dshi_3092 at 3261.285 kb on - strand, within Dshi_3092 at 3261.359 kb on + strand, within Dshi_3092 at 3261.422 kb on + strand, within Dshi_3092 at 3261.589 kb on - strand, within Dshi_3093 at 3262.039 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 3,259,212 - -0.5 3,260,036 + +1.5 3,260,115 + -1.3 3,260,115 + +0.7 3,260,128 - -1.2 3,260,267 + Dshi_3091 0.14 -2.3 3,260,267 + Dshi_3091 0.14 -0.6 3,260,353 - Dshi_3091 0.23 -2.6 3,260,398 + Dshi_3091 0.28 -1.0 3,260,659 - Dshi_3091 0.55 -1.6 3,260,708 + Dshi_3091 0.60 -1.6 3,260,746 + Dshi_3091 0.64 -2.2 3,260,757 + Dshi_3091 0.66 -0.8 3,260,772 + Dshi_3091 0.67 +0.7 3,261,093 - -0.8 3,261,237 - Dshi_3092 0.37 +0.6 3,261,285 - Dshi_3092 0.48 -0.3 3,261,359 + Dshi_3092 0.65 -0.5 3,261,422 + Dshi_3092 0.80 -1.1 3,261,589 - Dshi_3093 0.15 -0.3 3,262,039 - +0.4
Or see this region's nucleotide sequence