Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_R0049 and Dshi_2988 are separated by 259 nucleotides Dshi_2988 and Dshi_2989 are separated by 264 nucleotides Dshi_2989 and Dshi_2990 are separated by 155 nucleotides
Dshi_R0049: Dshi_R0049 - tRNA-Ala (RefSeq), at 3,153,418 to 3,153,493
_R0049
Dshi_2988: Dshi_2988 - protein of unknown function DUF74 (RefSeq), at 3,153,753 to 3,154,094
_2988
Dshi_2989: Dshi_2989 - hypothetical protein (RefSeq), at 3,154,359 to 3,154,565
_2989
Dshi_2990: Dshi_2990 - transcriptional regulator, LysR family (RefSeq), at 3,154,721 to 3,155,620
_2990
Position (kb)
3154
3155 Strain fitness (log2 ratio)
-2
-1
0
1 at 3153.677 kb on + strand at 3153.690 kb on - strand at 3153.690 kb on - strand at 3153.708 kb on + strand at 3153.744 kb on + strand at 3153.786 kb on - strand at 3153.786 kb on - strand at 3153.786 kb on - strand at 3153.808 kb on + strand, within Dshi_2988 at 3153.812 kb on + strand, within Dshi_2988 at 3153.813 kb on + strand, within Dshi_2988 at 3153.897 kb on + strand, within Dshi_2988 at 3153.969 kb on + strand, within Dshi_2988 at 3153.994 kb on + strand, within Dshi_2988 at 3154.059 kb on + strand, within Dshi_2988 at 3154.078 kb on + strand at 3154.078 kb on + strand at 3154.078 kb on + strand at 3154.078 kb on + strand at 3154.081 kb on - strand at 3154.086 kb on - strand at 3154.086 kb on - strand at 3154.086 kb on - strand at 3154.086 kb on - strand at 3154.086 kb on - strand at 3154.086 kb on - strand at 3154.132 kb on + strand at 3154.172 kb on - strand at 3154.217 kb on - strand at 3154.224 kb on + strand at 3154.253 kb on - strand at 3154.281 kb on + strand at 3154.320 kb on + strand at 3154.328 kb on - strand at 3154.328 kb on - strand at 3154.330 kb on + strand at 3154.338 kb on - strand at 3154.370 kb on - strand at 3154.416 kb on + strand, within Dshi_2989 at 3154.444 kb on - strand, within Dshi_2989 at 3154.444 kb on - strand, within Dshi_2989 at 3154.502 kb on - strand, within Dshi_2989 at 3154.519 kb on - strand, within Dshi_2989 at 3154.529 kb on - strand, within Dshi_2989 at 3154.620 kb on + strand at 3154.620 kb on + strand at 3154.639 kb on + strand at 3154.639 kb on + strand at 3154.715 kb on - strand at 3154.730 kb on + strand at 3154.746 kb on + strand at 3154.755 kb on - strand at 3154.762 kb on - strand at 3154.899 kb on - strand, within Dshi_2990 at 3154.979 kb on - strand, within Dshi_2990 at 3154.991 kb on - strand, within Dshi_2990 at 3155.024 kb on - strand, within Dshi_2990 at 3155.060 kb on - strand, within Dshi_2990 at 3155.081 kb on - strand, within Dshi_2990 at 3155.081 kb on - strand, within Dshi_2990 at 3155.099 kb on + strand, within Dshi_2990 at 3155.160 kb on - strand, within Dshi_2990 at 3155.160 kb on - strand at 3155.328 kb on + strand, within Dshi_2990 at 3155.360 kb on + strand, within Dshi_2990 at 3155.431 kb on - strand, within Dshi_2990 at 3155.540 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 3,153,677 + -1.4 3,153,690 - -1.0 3,153,690 - +0.2 3,153,708 + -0.8 3,153,744 + +0.3 3,153,786 - -0.6 3,153,786 - +0.6 3,153,786 - -0.1 3,153,808 + Dshi_2988 0.16 -0.5 3,153,812 + Dshi_2988 0.17 -0.3 3,153,813 + Dshi_2988 0.18 +0.2 3,153,897 + Dshi_2988 0.42 -1.8 3,153,969 + Dshi_2988 0.63 -0.4 3,153,994 + Dshi_2988 0.70 +1.2 3,154,059 + Dshi_2988 0.89 +0.6 3,154,078 + -0.6 3,154,078 + -0.6 3,154,078 + -0.1 3,154,078 + -0.2 3,154,081 - -1.9 3,154,086 - -1.8 3,154,086 - +0.9 3,154,086 - -0.4 3,154,086 - -0.4 3,154,086 - +0.6 3,154,086 - -0.2 3,154,132 + -1.4 3,154,172 - +0.0 3,154,217 - +0.2 3,154,224 + +0.3 3,154,253 - -0.4 3,154,281 + -0.1 3,154,320 + +0.1 3,154,328 - -0.8 3,154,328 - +0.4 3,154,330 + -0.4 3,154,338 - -0.0 3,154,370 - -2.6 3,154,416 + Dshi_2989 0.28 -0.8 3,154,444 - Dshi_2989 0.41 -0.1 3,154,444 - Dshi_2989 0.41 -1.1 3,154,502 - Dshi_2989 0.69 -0.8 3,154,519 - Dshi_2989 0.77 -0.4 3,154,529 - Dshi_2989 0.82 -0.4 3,154,620 + +0.7 3,154,620 + +0.4 3,154,639 + -1.4 3,154,639 + -0.2 3,154,715 - +0.8 3,154,730 + -0.6 3,154,746 + +0.9 3,154,755 - -0.0 3,154,762 - +1.2 3,154,899 - Dshi_2990 0.20 -0.2 3,154,979 - Dshi_2990 0.29 -0.2 3,154,991 - Dshi_2990 0.30 -0.3 3,155,024 - Dshi_2990 0.34 -0.8 3,155,060 - Dshi_2990 0.38 -0.8 3,155,081 - Dshi_2990 0.40 +0.6 3,155,081 - Dshi_2990 0.40 -0.4 3,155,099 + Dshi_2990 0.42 -1.8 3,155,160 - Dshi_2990 0.49 -0.6 3,155,160 - +0.6 3,155,328 + Dshi_2990 0.67 -1.7 3,155,360 + Dshi_2990 0.71 -0.5 3,155,431 - Dshi_2990 0.79 -0.5 3,155,540 + -0.4
Or see this region's nucleotide sequence