Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2566 and Dshi_2567 are separated by 397 nucleotides Dshi_2567 and Dshi_2568 overlap by 8 nucleotides Dshi_2568 and Dshi_2569 are separated by 8 nucleotides
Dshi_2566: Dshi_2566 - Transketolase central region (RefSeq), at 2,731,137 to 2,733,368
_2566
Dshi_2567: Dshi_2567 - multi-sensor signal transduction histidine kinase (RefSeq), at 2,733,766 to 2,736,072
_2567
Dshi_2568: Dshi_2568 - two component transcriptional regulator, LuxR family (RefSeq), at 2,736,065 to 2,736,709
_2568
Dshi_2569: Dshi_2569 - putative ammonia monooxygenase (RefSeq), at 2,736,718 to 2,737,746
_2569
Position (kb)
2733
2734
2735
2736
2737 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2732.848 kb on + strand, within Dshi_2566 at 2732.853 kb on + strand, within Dshi_2566 at 2732.883 kb on - strand, within Dshi_2566 at 2733.229 kb on - strand at 2733.250 kb on - strand at 2733.401 kb on + strand at 2733.428 kb on + strand at 2733.428 kb on + strand at 2733.436 kb on - strand at 2733.436 kb on - strand at 2733.480 kb on + strand at 2733.490 kb on + strand at 2733.514 kb on + strand at 2733.522 kb on - strand at 2733.634 kb on + strand at 2733.634 kb on + strand at 2733.719 kb on + strand at 2733.725 kb on - strand at 2733.727 kb on - strand at 2733.742 kb on + strand at 2733.756 kb on + strand at 2733.765 kb on + strand at 2733.805 kb on + strand at 2733.805 kb on + strand at 2733.857 kb on - strand at 2733.892 kb on + strand at 2733.931 kb on + strand at 2734.006 kb on + strand, within Dshi_2567 at 2734.017 kb on + strand, within Dshi_2567 at 2734.036 kb on + strand, within Dshi_2567 at 2734.051 kb on + strand, within Dshi_2567 at 2734.173 kb on + strand, within Dshi_2567 at 2734.179 kb on - strand, within Dshi_2567 at 2734.232 kb on + strand, within Dshi_2567 at 2734.232 kb on + strand, within Dshi_2567 at 2734.240 kb on - strand, within Dshi_2567 at 2734.240 kb on - strand, within Dshi_2567 at 2734.240 kb on - strand, within Dshi_2567 at 2734.240 kb on - strand, within Dshi_2567 at 2734.240 kb on - strand, within Dshi_2567 at 2734.240 kb on - strand, within Dshi_2567 at 2734.259 kb on + strand, within Dshi_2567 at 2734.265 kb on - strand, within Dshi_2567 at 2734.287 kb on + strand, within Dshi_2567 at 2734.295 kb on - strand, within Dshi_2567 at 2734.359 kb on + strand, within Dshi_2567 at 2734.367 kb on - strand, within Dshi_2567 at 2734.421 kb on - strand, within Dshi_2567 at 2734.441 kb on + strand, within Dshi_2567 at 2734.464 kb on - strand, within Dshi_2567 at 2734.488 kb on - strand, within Dshi_2567 at 2734.496 kb on - strand, within Dshi_2567 at 2734.521 kb on + strand, within Dshi_2567 at 2734.612 kb on - strand, within Dshi_2567 at 2734.618 kb on + strand, within Dshi_2567 at 2734.705 kb on + strand, within Dshi_2567 at 2734.795 kb on - strand, within Dshi_2567 at 2734.821 kb on - strand, within Dshi_2567 at 2734.832 kb on - strand, within Dshi_2567 at 2734.859 kb on + strand, within Dshi_2567 at 2734.859 kb on + strand, within Dshi_2567 at 2734.920 kb on - strand, within Dshi_2567 at 2734.925 kb on - strand, within Dshi_2567 at 2735.008 kb on + strand, within Dshi_2567 at 2735.069 kb on - strand, within Dshi_2567 at 2735.107 kb on - strand, within Dshi_2567 at 2735.211 kb on - strand, within Dshi_2567 at 2735.308 kb on - strand, within Dshi_2567 at 2735.329 kb on + strand, within Dshi_2567 at 2735.337 kb on - strand, within Dshi_2567 at 2735.337 kb on - strand, within Dshi_2567 at 2735.342 kb on - strand, within Dshi_2567 at 2735.362 kb on + strand, within Dshi_2567 at 2735.370 kb on - strand, within Dshi_2567 at 2735.447 kb on + strand, within Dshi_2567 at 2735.604 kb on + strand, within Dshi_2567 at 2735.670 kb on + strand, within Dshi_2567 at 2735.680 kb on + strand, within Dshi_2567 at 2735.714 kb on - strand, within Dshi_2567 at 2735.714 kb on - strand, within Dshi_2567 at 2735.720 kb on - strand, within Dshi_2567 at 2735.793 kb on - strand, within Dshi_2567 at 2735.800 kb on + strand, within Dshi_2567 at 2735.808 kb on - strand, within Dshi_2567 at 2735.889 kb on - strand at 2735.966 kb on + strand at 2735.966 kb on + strand at 2735.966 kb on + strand at 2735.966 kb on + strand at 2735.966 kb on + strand at 2735.966 kb on + strand at 2735.974 kb on - strand at 2736.027 kb on - strand at 2736.131 kb on - strand, within Dshi_2568 at 2736.136 kb on + strand, within Dshi_2568 at 2736.143 kb on + strand, within Dshi_2568 at 2736.218 kb on + strand, within Dshi_2568 at 2736.218 kb on + strand, within Dshi_2568 at 2736.226 kb on - strand, within Dshi_2568 at 2736.226 kb on - strand, within Dshi_2568 at 2736.226 kb on - strand, within Dshi_2568 at 2736.226 kb on - strand, within Dshi_2568 at 2736.503 kb on - strand, within Dshi_2568 at 2736.672 kb on - strand at 2736.691 kb on + strand at 2736.691 kb on + strand at 2736.691 kb on + strand at 2736.699 kb on - strand at 2736.699 kb on - strand at 2736.699 kb on - strand at 2736.709 kb on + strand at 2736.711 kb on + strand at 2736.789 kb on + strand at 2736.811 kb on + strand at 2736.819 kb on - strand at 2736.905 kb on - strand, within Dshi_2569
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 2,732,848 + Dshi_2566 0.77 -1.4 2,732,853 + Dshi_2566 0.77 -0.2 2,732,883 - Dshi_2566 0.78 +0.8 2,733,229 - -0.7 2,733,250 - -0.9 2,733,401 + -0.1 2,733,428 + +0.3 2,733,428 + -0.9 2,733,436 - +0.2 2,733,436 - +0.9 2,733,480 + +1.7 2,733,490 + +1.3 2,733,514 + +0.3 2,733,522 - -1.8 2,733,634 + +0.0 2,733,634 + +0.3 2,733,719 + +1.1 2,733,725 - -0.2 2,733,727 - -0.5 2,733,742 + -0.3 2,733,756 + +0.1 2,733,765 + -0.3 2,733,805 + -1.5 2,733,805 + -1.3 2,733,857 - -1.6 2,733,892 + -0.0 2,733,931 + -0.0 2,734,006 + Dshi_2567 0.10 -1.0 2,734,017 + Dshi_2567 0.11 -0.6 2,734,036 + Dshi_2567 0.12 -0.3 2,734,051 + Dshi_2567 0.12 -1.0 2,734,173 + Dshi_2567 0.18 +0.1 2,734,179 - Dshi_2567 0.18 +1.0 2,734,232 + Dshi_2567 0.20 -2.8 2,734,232 + Dshi_2567 0.20 -1.8 2,734,240 - Dshi_2567 0.21 +0.3 2,734,240 - Dshi_2567 0.21 -0.6 2,734,240 - Dshi_2567 0.21 +0.6 2,734,240 - Dshi_2567 0.21 -1.5 2,734,240 - Dshi_2567 0.21 +0.0 2,734,240 - Dshi_2567 0.21 -0.7 2,734,259 + Dshi_2567 0.21 -0.2 2,734,265 - Dshi_2567 0.22 +0.1 2,734,287 + Dshi_2567 0.23 -0.4 2,734,295 - Dshi_2567 0.23 -0.0 2,734,359 + Dshi_2567 0.26 -1.6 2,734,367 - Dshi_2567 0.26 -0.0 2,734,421 - Dshi_2567 0.28 +0.3 2,734,441 + Dshi_2567 0.29 -1.4 2,734,464 - Dshi_2567 0.30 -1.4 2,734,488 - Dshi_2567 0.31 -0.2 2,734,496 - Dshi_2567 0.32 -1.7 2,734,521 + Dshi_2567 0.33 -0.3 2,734,612 - Dshi_2567 0.37 -0.1 2,734,618 + Dshi_2567 0.37 -1.4 2,734,705 + Dshi_2567 0.41 -0.3 2,734,795 - Dshi_2567 0.45 +0.5 2,734,821 - Dshi_2567 0.46 -1.2 2,734,832 - Dshi_2567 0.46 +0.2 2,734,859 + Dshi_2567 0.47 +0.1 2,734,859 + Dshi_2567 0.47 -0.7 2,734,920 - Dshi_2567 0.50 -2.0 2,734,925 - Dshi_2567 0.50 +0.3 2,735,008 + Dshi_2567 0.54 -2.0 2,735,069 - Dshi_2567 0.56 -1.4 2,735,107 - Dshi_2567 0.58 -1.3 2,735,211 - Dshi_2567 0.63 +1.0 2,735,308 - Dshi_2567 0.67 -1.0 2,735,329 + Dshi_2567 0.68 -1.4 2,735,337 - Dshi_2567 0.68 -0.0 2,735,337 - Dshi_2567 0.68 +0.3 2,735,342 - Dshi_2567 0.68 -0.1 2,735,362 + Dshi_2567 0.69 -1.6 2,735,370 - Dshi_2567 0.70 -0.0 2,735,447 + Dshi_2567 0.73 -0.1 2,735,604 + Dshi_2567 0.80 -2.5 2,735,670 + Dshi_2567 0.83 -0.0 2,735,680 + Dshi_2567 0.83 -2.0 2,735,714 - Dshi_2567 0.84 -1.4 2,735,714 - Dshi_2567 0.84 -2.0 2,735,720 - Dshi_2567 0.85 +2.1 2,735,793 - Dshi_2567 0.88 -0.9 2,735,800 + Dshi_2567 0.88 -0.5 2,735,808 - Dshi_2567 0.89 -0.9 2,735,889 - +2.1 2,735,966 + -2.0 2,735,966 + -1.0 2,735,966 + -1.6 2,735,966 + -1.0 2,735,966 + -0.3 2,735,966 + -1.9 2,735,974 - -2.2 2,736,027 - +0.3 2,736,131 - Dshi_2568 0.10 -0.4 2,736,136 + Dshi_2568 0.11 -0.6 2,736,143 + Dshi_2568 0.12 -0.2 2,736,218 + Dshi_2568 0.24 -0.7 2,736,218 + Dshi_2568 0.24 -0.5 2,736,226 - Dshi_2568 0.25 +0.1 2,736,226 - Dshi_2568 0.25 -0.4 2,736,226 - Dshi_2568 0.25 -0.5 2,736,226 - Dshi_2568 0.25 +1.0 2,736,503 - Dshi_2568 0.68 -0.3 2,736,672 - +0.4 2,736,691 + +1.0 2,736,691 + -0.7 2,736,691 + -0.1 2,736,699 - -0.1 2,736,699 - -1.2 2,736,699 - -0.3 2,736,709 + +1.3 2,736,711 + +0.1 2,736,789 + -0.2 2,736,811 + +0.3 2,736,819 - +1.9 2,736,905 - Dshi_2569 0.18 -1.2
Or see this region's nucleotide sequence