Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2197 and Dshi_2198 are separated by 79 nucleotides Dshi_2198 and Dshi_2199 are separated by 218 nucleotides Dshi_2199 and Dshi_2200 are separated by 77 nucleotides
Dshi_2197: Dshi_2197 - ABC transporter related (RefSeq), at 2,324,302 to 2,325,957
_2197
Dshi_2198: Dshi_2198 - MOSC domain containing protein (RefSeq), at 2,326,037 to 2,326,516
_2198
Dshi_2199: Dshi_2199 - aspartate racemase (RefSeq), at 2,326,735 to 2,327,424
_2199
Dshi_2200: Dshi_2200 - regulatory protein TetR (RefSeq), at 2,327,502 to 2,328,092
_2200
Position (kb)
2326
2327 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2325.037 kb on + strand, within Dshi_2197 at 2325.451 kb on - strand, within Dshi_2197 at 2325.461 kb on - strand, within Dshi_2197 at 2325.533 kb on + strand, within Dshi_2197 at 2325.555 kb on + strand, within Dshi_2197 at 2325.598 kb on + strand, within Dshi_2197 at 2325.696 kb on + strand, within Dshi_2197 at 2325.766 kb on - strand, within Dshi_2197 at 2325.766 kb on - strand, within Dshi_2197 at 2325.804 kb on + strand at 2325.869 kb on - strand at 2325.954 kb on + strand at 2325.969 kb on + strand at 2325.995 kb on + strand at 2326.003 kb on - strand at 2326.003 kb on - strand at 2326.018 kb on + strand at 2326.084 kb on + strand at 2326.142 kb on + strand, within Dshi_2198 at 2326.258 kb on + strand, within Dshi_2198 at 2326.271 kb on + strand, within Dshi_2198 at 2326.372 kb on - strand, within Dshi_2198 at 2326.607 kb on - strand at 2326.712 kb on + strand at 2326.785 kb on + strand at 2326.790 kb on + strand at 2326.798 kb on - strand at 2326.798 kb on - strand at 2326.827 kb on - strand, within Dshi_2199 at 2326.908 kb on - strand, within Dshi_2199 at 2326.921 kb on + strand, within Dshi_2199 at 2326.931 kb on + strand, within Dshi_2199 at 2327.017 kb on - strand, within Dshi_2199 at 2327.059 kb on + strand, within Dshi_2199 at 2327.216 kb on + strand, within Dshi_2199 at 2327.226 kb on + strand, within Dshi_2199 at 2327.235 kb on + strand, within Dshi_2199 at 2327.344 kb on + strand, within Dshi_2199 at 2327.385 kb on + strand at 2327.440 kb on - strand at 2327.503 kb on - strand at 2327.503 kb on - strand at 2327.503 kb on - strand at 2327.509 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 2,325,037 + Dshi_2197 0.44 -3.1 2,325,451 - Dshi_2197 0.69 -1.0 2,325,461 - Dshi_2197 0.70 -0.2 2,325,533 + Dshi_2197 0.74 -0.3 2,325,555 + Dshi_2197 0.76 -1.5 2,325,598 + Dshi_2197 0.78 -0.8 2,325,696 + Dshi_2197 0.84 -2.0 2,325,766 - Dshi_2197 0.88 -1.9 2,325,766 - Dshi_2197 0.88 +0.8 2,325,804 + -0.8 2,325,869 - -1.6 2,325,954 + -0.4 2,325,969 + -0.6 2,325,995 + -1.8 2,326,003 - +0.4 2,326,003 - -0.3 2,326,018 + -0.0 2,326,084 + -1.4 2,326,142 + Dshi_2198 0.22 -1.9 2,326,258 + Dshi_2198 0.46 -0.3 2,326,271 + Dshi_2198 0.49 +0.3 2,326,372 - Dshi_2198 0.70 -1.4 2,326,607 - -1.3 2,326,712 + +0.2 2,326,785 + +0.1 2,326,790 + -0.7 2,326,798 - +0.9 2,326,798 - -0.5 2,326,827 - Dshi_2199 0.13 -0.1 2,326,908 - Dshi_2199 0.25 -0.2 2,326,921 + Dshi_2199 0.27 -0.5 2,326,931 + Dshi_2199 0.28 -0.6 2,327,017 - Dshi_2199 0.41 +3.1 2,327,059 + Dshi_2199 0.47 +0.4 2,327,216 + Dshi_2199 0.70 -0.5 2,327,226 + Dshi_2199 0.71 -0.4 2,327,235 + Dshi_2199 0.72 +0.5 2,327,344 + Dshi_2199 0.88 +0.2 2,327,385 + -0.1 2,327,440 - -1.8 2,327,503 - +0.1 2,327,503 - -0.0 2,327,503 - -0.0 2,327,509 + -0.4
Or see this region's nucleotide sequence