Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1592 and Dshi_1593 are separated by 35 nucleotides Dshi_1593 and Dshi_1594 overlap by 26 nucleotides Dshi_1594 and Dshi_1595 are separated by 81 nucleotides Dshi_1595 and Dshi_1596 overlap by 8 nucleotides
Dshi_1592: Dshi_1592 - hypothetical protein (RefSeq), at 1,658,518 to 1,659,390
_1592
Dshi_1593: Dshi_1593 - Homoserine O-succinyltransferase (RefSeq), at 1,659,426 to 1,660,343
_1593
Dshi_1594: Dshi_1594 - alpha/beta hydrolase fold (RefSeq), at 1,660,318 to 1,661,280
_1594
Dshi_1595: Dshi_1595 - protein of unknown function DUF344 (RefSeq), at 1,661,362 to 1,662,231
_1595
Dshi_1596: Dshi_1596 - transcriptional regulator, TetR family (RefSeq), at 1,662,224 to 1,662,841
_1596
Position (kb)
1660
1661
1662 Strain fitness (log2 ratio)
-1
0
1 at 1660.446 kb on + strand, within Dshi_1594 at 1660.606 kb on + strand, within Dshi_1594 at 1660.741 kb on + strand, within Dshi_1594 at 1660.831 kb on + strand, within Dshi_1594 at 1660.935 kb on + strand, within Dshi_1594 at 1661.002 kb on + strand, within Dshi_1594 at 1661.002 kb on + strand, within Dshi_1594 at 1661.010 kb on - strand, within Dshi_1594 at 1661.031 kb on - strand, within Dshi_1594 at 1661.195 kb on - strand at 1661.324 kb on + strand at 1661.333 kb on + strand at 1661.341 kb on + strand at 1661.349 kb on - strand at 1661.516 kb on + strand, within Dshi_1595 at 1661.556 kb on - strand, within Dshi_1595 at 1661.628 kb on + strand, within Dshi_1595 at 1661.628 kb on + strand, within Dshi_1595 at 1661.656 kb on - strand, within Dshi_1595 at 1661.690 kb on - strand, within Dshi_1595 at 1661.896 kb on - strand, within Dshi_1595 at 1662.146 kb on - strand at 1662.172 kb on - strand at 1662.206 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 1,660,446 + Dshi_1594 0.13 -1.5 1,660,606 + Dshi_1594 0.30 -1.6 1,660,741 + Dshi_1594 0.44 -1.3 1,660,831 + Dshi_1594 0.53 -0.3 1,660,935 + Dshi_1594 0.64 +0.5 1,661,002 + Dshi_1594 0.71 -1.6 1,661,002 + Dshi_1594 0.71 -0.1 1,661,010 - Dshi_1594 0.72 -0.3 1,661,031 - Dshi_1594 0.74 -0.9 1,661,195 - -0.2 1,661,324 + +0.2 1,661,333 + +0.1 1,661,341 + +1.2 1,661,349 - -0.9 1,661,516 + Dshi_1595 0.18 +0.7 1,661,556 - Dshi_1595 0.22 +0.3 1,661,628 + Dshi_1595 0.31 -0.1 1,661,628 + Dshi_1595 0.31 +0.4 1,661,656 - Dshi_1595 0.34 +0.0 1,661,690 - Dshi_1595 0.38 -0.2 1,661,896 - Dshi_1595 0.61 -1.5 1,662,146 - -0.0 1,662,172 - +0.4 1,662,206 - -0.9
Or see this region's nucleotide sequence