Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1469 and Dshi_1470 are separated by 14 nucleotides Dshi_1470 and Dshi_1471 are separated by 29 nucleotides Dshi_1471 and Dshi_1472 are separated by 120 nucleotides Dshi_1472 and Dshi_1473 overlap by 4 nucleotides Dshi_1473 and Dshi_1474 overlap by 37 nucleotides
Dshi_1469: Dshi_1469 - gamma-glutamyl phosphate reductase (RefSeq), at 1,523,915 to 1,525,180
_1469
Dshi_1470: Dshi_1470 - hypothetical protein (RefSeq), at 1,525,195 to 1,525,809
_1470
Dshi_1471: Dshi_1471 - hypothetical protein (RefSeq), at 1,525,839 to 1,526,024
_1471
Dshi_1472: Dshi_1472 - phospholipid/glycerol acyltransferase (RefSeq), at 1,526,145 to 1,526,921
_1472
Dshi_1473: Dshi_1473 - phospholipid/glycerol acyltransferase (RefSeq), at 1,526,918 to 1,527,763
_1473
Dshi_1474: Dshi_1474 - glutamyl-tRNA synthetase class Ic (RefSeq), at 1,527,727 to 1,528,590
_1474
Position (kb)
1526
1527 Strain fitness (log2 ratio)
-1
0
1 at 1525.323 kb on + strand, within Dshi_1470 at 1525.528 kb on - strand, within Dshi_1470 at 1525.545 kb on - strand, within Dshi_1470 at 1525.643 kb on - strand, within Dshi_1470 at 1525.680 kb on + strand, within Dshi_1470 at 1525.688 kb on - strand, within Dshi_1470 at 1525.807 kb on - strand at 1525.807 kb on - strand at 1525.807 kb on - strand at 1526.061 kb on + strand at 1526.140 kb on + strand at 1526.565 kb on + strand, within Dshi_1472 at 1526.735 kb on + strand, within Dshi_1472 at 1526.782 kb on - strand, within Dshi_1472 at 1526.861 kb on + strand at 1526.878 kb on + strand at 1526.886 kb on - strand at 1526.957 kb on - strand at 1527.169 kb on + strand, within Dshi_1473 at 1527.517 kb on + strand, within Dshi_1473 at 1527.684 kb on + strand at 1527.917 kb on + strand, within Dshi_1474
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 1,525,323 + Dshi_1470 0.21 +1.4 1,525,528 - Dshi_1470 0.54 +0.3 1,525,545 - Dshi_1470 0.57 -1.0 1,525,643 - Dshi_1470 0.73 -0.1 1,525,680 + Dshi_1470 0.79 -0.7 1,525,688 - Dshi_1470 0.80 -0.9 1,525,807 - +0.7 1,525,807 - +0.3 1,525,807 - -0.3 1,526,061 + -0.1 1,526,140 + +0.2 1,526,565 + Dshi_1472 0.54 -0.7 1,526,735 + Dshi_1472 0.76 -0.6 1,526,782 - Dshi_1472 0.82 -1.0 1,526,861 + -1.1 1,526,878 + -0.6 1,526,886 - -0.3 1,526,957 - -0.6 1,527,169 + Dshi_1473 0.30 -0.5 1,527,517 + Dshi_1473 0.71 +0.8 1,527,684 + +0.2 1,527,917 + Dshi_1474 0.22 -0.2
Or see this region's nucleotide sequence