Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1235 and Dshi_1236 overlap by 37 nucleotides Dshi_1236 and Dshi_1237 are separated by 3 nucleotides Dshi_1237 and Dshi_1238 are separated by 82 nucleotides
Dshi_1235: Dshi_1235 - xylulokinase (RefSeq), at 1,279,366 to 1,280,808
_1235
Dshi_1236: Dshi_1236 - DMSO reductase anchor subunit (DmsC) (RefSeq), at 1,280,772 to 1,281,659
_1236
Dshi_1237: Dshi_1237 - 4Fe-4S ferredoxin iron-sulfur binding domain protein (RefSeq), at 1,281,663 to 1,282,409
_1237
Dshi_1238: Dshi_1238 - molybdopterin oxidoreductase Fe4S4 region (RefSeq), at 1,282,492 to 1,285,278
_1238
Position (kb)
1280
1281
1282 Strain fitness (log2 ratio)
-2
-1
0
1 at 1280.620 kb on - strand, within Dshi_1235 at 1280.824 kb on + strand at 1280.896 kb on + strand, within Dshi_1236 at 1280.966 kb on - strand, within Dshi_1236 at 1281.141 kb on - strand, within Dshi_1236 at 1281.333 kb on - strand, within Dshi_1236 at 1281.539 kb on - strand, within Dshi_1236 at 1281.576 kb on - strand at 1281.576 kb on - strand at 1281.627 kb on + strand at 1281.669 kb on - strand at 1281.679 kb on - strand at 1281.682 kb on + strand at 1281.769 kb on - strand, within Dshi_1237 at 1281.918 kb on + strand, within Dshi_1237 at 1282.046 kb on - strand, within Dshi_1237 at 1282.198 kb on - strand, within Dshi_1237 at 1282.214 kb on + strand, within Dshi_1237 at 1282.291 kb on + strand, within Dshi_1237 at 1282.291 kb on + strand, within Dshi_1237 at 1282.291 kb on + strand, within Dshi_1237 at 1282.291 kb on + strand, within Dshi_1237 at 1282.313 kb on - strand, within Dshi_1237 at 1282.450 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 1,280,620 - Dshi_1235 0.87 +0.0 1,280,824 + -0.0 1,280,896 + Dshi_1236 0.14 -0.7 1,280,966 - Dshi_1236 0.22 -1.6 1,281,141 - Dshi_1236 0.42 +0.2 1,281,333 - Dshi_1236 0.63 -0.4 1,281,539 - Dshi_1236 0.86 -1.0 1,281,576 - +0.2 1,281,576 - -0.4 1,281,627 + +0.1 1,281,669 - -0.0 1,281,679 - -0.2 1,281,682 + +1.0 1,281,769 - Dshi_1237 0.14 +1.1 1,281,918 + Dshi_1237 0.34 -0.4 1,282,046 - Dshi_1237 0.51 -0.0 1,282,198 - Dshi_1237 0.72 -0.8 1,282,214 + Dshi_1237 0.74 -0.8 1,282,291 + Dshi_1237 0.84 +0.5 1,282,291 + Dshi_1237 0.84 +1.0 1,282,291 + Dshi_1237 0.84 +0.5 1,282,291 + Dshi_1237 0.84 +0.3 1,282,313 - Dshi_1237 0.87 -2.1 1,282,450 - +0.1
Or see this region's nucleotide sequence