Strain Fitness in Dinoroseobacter shibae DFL-12 around Dshi_0195

Experiment: L-Alanine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntDshi_0194 and Dshi_0195 are separated by 37 nucleotidesDshi_0195 and Dshi_0196 are separated by 25 nucleotidesDshi_0196 and Dshi_0197 overlap by 4 nucleotides Dshi_0194: Dshi_0194 - (p)ppGpp synthetase I, SpoT/RelA (RefSeq), at 193,417 to 195,561 _0194 Dshi_0195: Dshi_0195 - hypothetical protein (RefSeq), at 195,599 to 196,255 _0195 Dshi_0196: Dshi_0196 - pyridoxal phosphate biosynthetic protein PdxJ (RefSeq), at 196,281 to 197,045 _0196 Dshi_0197: Dshi_0197 - hypothetical protein (RefSeq), at 197,042 to 197,704 _0197 Position (kb) 195 196 197Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 194.609 kb on + strand, within Dshi_0194at 194.615 kb on + strand, within Dshi_0194at 194.648 kb on - strand, within Dshi_0194at 194.697 kb on + strand, within Dshi_0194at 194.709 kb on + strand, within Dshi_0194at 194.717 kb on - strand, within Dshi_0194at 194.748 kb on + strand, within Dshi_0194at 194.879 kb on - strand, within Dshi_0194at 194.880 kb on - strand, within Dshi_0194at 195.010 kb on + strand, within Dshi_0194at 195.010 kb on + strand, within Dshi_0194at 195.010 kb on + strand, within Dshi_0194at 195.010 kb on + strand, within Dshi_0194at 195.010 kb on + strand, within Dshi_0194at 195.104 kb on + strand, within Dshi_0194at 195.200 kb on + strand, within Dshi_0194at 195.208 kb on - strand, within Dshi_0194at 195.219 kb on - strand, within Dshi_0194at 195.290 kb on + strand, within Dshi_0194at 195.316 kb on + strand, within Dshi_0194at 195.454 kb on + strandat 195.460 kb on - strandat 195.475 kb on - strandat 195.475 kb on - strandat 195.549 kb on - strandat 195.554 kb on + strandat 195.562 kb on - strandat 195.666 kb on - strand, within Dshi_0195at 195.666 kb on - strand, within Dshi_0195at 195.678 kb on + strand, within Dshi_0195at 195.887 kb on - strand, within Dshi_0195at 195.887 kb on - strand, within Dshi_0195at 195.904 kb on + strand, within Dshi_0195at 195.912 kb on - strand, within Dshi_0195at 195.957 kb on + strand, within Dshi_0195at 195.963 kb on - strand, within Dshi_0195at 195.972 kb on + strand, within Dshi_0195at 195.990 kb on - strand, within Dshi_0195at 195.995 kb on + strand, within Dshi_0195at 196.072 kb on + strand, within Dshi_0195at 196.080 kb on - strand, within Dshi_0195at 196.080 kb on - strand, within Dshi_0195at 196.080 kb on - strand, within Dshi_0195at 196.142 kb on - strand, within Dshi_0195at 196.171 kb on + strand, within Dshi_0195at 196.295 kb on - strandat 197.024 kb on + strandat 197.039 kb on + strandat 197.057 kb on - strandat 197.092 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Alanine (N)
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194,609 + Dshi_0194 0.56 -3.1
194,615 + Dshi_0194 0.56 -1.1
194,648 - Dshi_0194 0.57 -2.0
194,697 + Dshi_0194 0.60 -4.1
194,709 + Dshi_0194 0.60 -1.1
194,717 - Dshi_0194 0.61 -2.4
194,748 + Dshi_0194 0.62 -1.7
194,879 - Dshi_0194 0.68 -1.0
194,880 - Dshi_0194 0.68 -0.4
195,010 + Dshi_0194 0.74 -0.6
195,010 + Dshi_0194 0.74 -0.2
195,010 + Dshi_0194 0.74 -0.1
195,010 + Dshi_0194 0.74 -1.2
195,010 + Dshi_0194 0.74 -0.3
195,104 + Dshi_0194 0.79 +0.2
195,200 + Dshi_0194 0.83 +1.4
195,208 - Dshi_0194 0.83 -0.9
195,219 - Dshi_0194 0.84 -0.6
195,290 + Dshi_0194 0.87 -0.1
195,316 + Dshi_0194 0.89 +0.4
195,454 + -0.9
195,460 - -0.5
195,475 - -0.1
195,475 - -0.7
195,549 - -0.8
195,554 + +1.3
195,562 - +1.2
195,666 - Dshi_0195 0.10 +0.7
195,666 - Dshi_0195 0.10 +0.9
195,678 + Dshi_0195 0.12 -0.2
195,887 - Dshi_0195 0.44 +0.9
195,887 - Dshi_0195 0.44 +0.5
195,904 + Dshi_0195 0.46 +1.0
195,912 - Dshi_0195 0.48 -0.6
195,957 + Dshi_0195 0.54 +0.6
195,963 - Dshi_0195 0.55 +3.0
195,972 + Dshi_0195 0.57 +0.8
195,990 - Dshi_0195 0.60 +0.3
195,995 + Dshi_0195 0.60 +0.8
196,072 + Dshi_0195 0.72 +0.4
196,080 - Dshi_0195 0.73 +0.4
196,080 - Dshi_0195 0.73 -0.2
196,080 - Dshi_0195 0.73 +0.3
196,142 - Dshi_0195 0.83 +0.7
196,171 + Dshi_0195 0.87 +0.8
196,295 - -1.2
197,024 + +0.0
197,039 + -0.3
197,057 - -0.3
197,092 - +0.1

Or see this region's nucleotide sequence