Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2039 and Dshi_2040 are separated by 46 nucleotides Dshi_2040 and Dshi_5005 overlap by 17 nucleotides Dshi_5005 and Dshi_2042 are separated by 132 nucleotides
Dshi_2039: Dshi_2039 - Malate/L-lactate dehydrogenase (RefSeq), at 2,158,316 to 2,159,335
_2039
Dshi_2040: Dshi_2040 - UspA domain protein (RefSeq), at 2,159,382 to 2,159,789
_2040
Dshi_5005: Dshi_5005 - hypothetical protein (RefSeq), at 2,159,773 to 2,160,468
_5005
Dshi_2042: Dshi_2042 - aminotransferase class-III (RefSeq), at 2,160,601 to 2,161,983
_2042
Position (kb)
2159
2160
2161 Strain fitness (log2 ratio)
-2
-1
0
1 at 2158.817 kb on + strand, within Dshi_2039 at 2158.830 kb on - strand, within Dshi_2039 at 2158.864 kb on + strand, within Dshi_2039 at 2158.864 kb on + strand, within Dshi_2039 at 2159.180 kb on - strand, within Dshi_2039 at 2159.516 kb on - strand, within Dshi_2040 at 2159.552 kb on - strand, within Dshi_2040 at 2159.621 kb on + strand, within Dshi_2040 at 2159.624 kb on + strand, within Dshi_2040 at 2159.679 kb on + strand, within Dshi_2040 at 2159.679 kb on + strand, within Dshi_2040 at 2159.790 kb on + strand at 2160.099 kb on + strand, within Dshi_5005 at 2160.188 kb on + strand, within Dshi_5005 at 2160.285 kb on + strand, within Dshi_5005 at 2160.345 kb on + strand, within Dshi_5005 at 2160.377 kb on - strand, within Dshi_5005 at 2160.471 kb on - strand at 2160.529 kb on + strand at 2160.619 kb on + strand at 2160.627 kb on - strand at 2160.838 kb on + strand, within Dshi_2042 at 2160.909 kb on - strand, within Dshi_2042 at 2160.909 kb on - strand, within Dshi_2042 at 2161.000 kb on - strand, within Dshi_2042 at 2161.052 kb on + strand, within Dshi_2042 at 2161.060 kb on + strand, within Dshi_2042 at 2161.060 kb on - strand, within Dshi_2042 at 2161.087 kb on - strand, within Dshi_2042 at 2161.087 kb on - strand, within Dshi_2042 at 2161.116 kb on - strand, within Dshi_2042 at 2161.316 kb on - strand, within Dshi_2042 at 2161.385 kb on + strand, within Dshi_2042 at 2161.390 kb on + strand, within Dshi_2042 at 2161.397 kb on + strand, within Dshi_2042 at 2161.398 kb on - strand, within Dshi_2042 at 2161.401 kb on + strand, within Dshi_2042
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 2,158,817 + Dshi_2039 0.49 +0.4 2,158,830 - Dshi_2039 0.50 +0.3 2,158,864 + Dshi_2039 0.54 -0.3 2,158,864 + Dshi_2039 0.54 +0.2 2,159,180 - Dshi_2039 0.85 -0.1 2,159,516 - Dshi_2040 0.33 -0.8 2,159,552 - Dshi_2040 0.42 -2.1 2,159,621 + Dshi_2040 0.59 +0.3 2,159,624 + Dshi_2040 0.59 +0.2 2,159,679 + Dshi_2040 0.73 -0.6 2,159,679 + Dshi_2040 0.73 +0.7 2,159,790 + +1.1 2,160,099 + Dshi_5005 0.47 -0.4 2,160,188 + Dshi_5005 0.60 -0.9 2,160,285 + Dshi_5005 0.74 -1.3 2,160,345 + Dshi_5005 0.82 +0.4 2,160,377 - Dshi_5005 0.87 -0.9 2,160,471 - -1.5 2,160,529 + +0.1 2,160,619 + +0.2 2,160,627 - -0.8 2,160,838 + Dshi_2042 0.17 -0.5 2,160,909 - Dshi_2042 0.22 +0.7 2,160,909 - Dshi_2042 0.22 -0.2 2,161,000 - Dshi_2042 0.29 -0.1 2,161,052 + Dshi_2042 0.33 +0.0 2,161,060 + Dshi_2042 0.33 +0.3 2,161,060 - Dshi_2042 0.33 -0.3 2,161,087 - Dshi_2042 0.35 +0.2 2,161,087 - Dshi_2042 0.35 +0.7 2,161,116 - Dshi_2042 0.37 +0.5 2,161,316 - Dshi_2042 0.52 +0.9 2,161,385 + Dshi_2042 0.57 -0.8 2,161,390 + Dshi_2042 0.57 +0.3 2,161,397 + Dshi_2042 0.58 -0.9 2,161,398 - Dshi_2042 0.58 -0.1 2,161,401 + Dshi_2042 0.58 -0.3
Or see this region's nucleotide sequence