Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS23860

Experiment: Plant=Tomato_cv_Moneymaker; PlantTreatment=None; Sample=Xylem_sap; GrowthSubstrate=calcine_clay; Collection=direct; 1 dpi

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntRALBFv3_RS23855 and RALBFv3_RS23860 are separated by 53 nucleotidesRALBFv3_RS23860 and RALBFv3_RS09350 overlap by 62 nucleotides RALBFv3_RS23855: RALBFv3_RS23855 - hypothetical protein, at 2,071,583 to 2,072,950 _RS23855 RALBFv3_RS23860: RALBFv3_RS23860 - hypothetical protein, at 2,073,004 to 2,074,131 _RS23860 RALBFv3_RS09350: RALBFv3_RS09350 - TniB family protein, at 2,074,070 to 2,075,095 _RS09350 Position (kb) 2073 2074 2075Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2072.067 kb on + strand, within RALBFv3_RS23855at 2072.067 kb on + strand, within RALBFv3_RS23855at 2072.067 kb on + strand, within RALBFv3_RS23855at 2072.067 kb on + strand, within RALBFv3_RS23855at 2072.068 kb on - strand, within RALBFv3_RS23855at 2072.068 kb on - strand, within RALBFv3_RS23855at 2072.068 kb on - strand, within RALBFv3_RS23855at 2072.068 kb on - strand, within RALBFv3_RS23855at 2072.068 kb on - strand, within RALBFv3_RS23855at 2072.068 kb on - strand, within RALBFv3_RS23855at 2072.068 kb on - strand, within RALBFv3_RS23855at 2072.070 kb on + strand, within RALBFv3_RS23855at 2072.070 kb on + strand, within RALBFv3_RS23855at 2072.129 kb on - strand, within RALBFv3_RS23855at 2072.133 kb on + strand, within RALBFv3_RS23855at 2072.149 kb on - strand, within RALBFv3_RS23855at 2072.331 kb on + strand, within RALBFv3_RS23855at 2072.332 kb on - strand, within RALBFv3_RS23855at 2072.350 kb on + strand, within RALBFv3_RS23855at 2072.441 kb on + strand, within RALBFv3_RS23855at 2072.441 kb on + strand, within RALBFv3_RS23855at 2072.442 kb on - strand, within RALBFv3_RS23855at 2072.451 kb on + strand, within RALBFv3_RS23855at 2072.452 kb on - strand, within RALBFv3_RS23855at 2072.499 kb on - strand, within RALBFv3_RS23855at 2072.499 kb on - strand, within RALBFv3_RS23855at 2072.499 kb on - strand, within RALBFv3_RS23855at 2072.530 kb on + strand, within RALBFv3_RS23855at 2072.530 kb on + strand, within RALBFv3_RS23855at 2072.530 kb on + strand, within RALBFv3_RS23855at 2072.530 kb on + strand, within RALBFv3_RS23855at 2072.531 kb on - strand, within RALBFv3_RS23855at 2072.543 kb on + strand, within RALBFv3_RS23855at 2072.544 kb on - strand, within RALBFv3_RS23855at 2072.544 kb on - strand, within RALBFv3_RS23855at 2072.544 kb on - strand, within RALBFv3_RS23855at 2072.668 kb on - strand, within RALBFv3_RS23855at 2072.668 kb on - strand, within RALBFv3_RS23855at 2072.668 kb on - strand, within RALBFv3_RS23855at 2072.669 kb on - strand, within RALBFv3_RS23855at 2072.691 kb on + strand, within RALBFv3_RS23855at 2072.692 kb on - strand, within RALBFv3_RS23855at 2072.722 kb on + strand, within RALBFv3_RS23855at 2072.744 kb on + strand, within RALBFv3_RS23855at 2072.744 kb on + strand, within RALBFv3_RS23855at 2072.744 kb on + strand, within RALBFv3_RS23855at 2072.745 kb on - strand, within RALBFv3_RS23855at 2072.745 kb on - strand, within RALBFv3_RS23855at 2072.765 kb on + strand, within RALBFv3_RS23855at 2072.765 kb on + strand, within RALBFv3_RS23855at 2072.765 kb on + strand, within RALBFv3_RS23855at 2072.766 kb on - strand, within RALBFv3_RS23855at 2072.766 kb on - strand, within RALBFv3_RS23855at 2072.766 kb on - strand, within RALBFv3_RS23855at 2072.766 kb on - strand, within RALBFv3_RS23855at 2072.766 kb on - strand, within RALBFv3_RS23855at 2072.766 kb on - strand, within RALBFv3_RS23855at 2072.770 kb on + strand, within RALBFv3_RS23855at 2072.771 kb on - strand, within RALBFv3_RS23855at 2072.876 kb on + strandat 2072.876 kb on + strandat 2072.898 kb on + strandat 2072.898 kb on + strandat 2072.899 kb on - strandat 2072.942 kb on + strandat 2072.973 kb on - strandat 2072.973 kb on - strandat 2073.047 kb on - strandat 2073.081 kb on + strandat 2073.082 kb on - strandat 2073.125 kb on + strand, within RALBFv3_RS23860at 2073.155 kb on - strand, within RALBFv3_RS23860at 2073.214 kb on - strand, within RALBFv3_RS23860at 2073.282 kb on - strand, within RALBFv3_RS23860at 2073.301 kb on + strand, within RALBFv3_RS23860at 2073.302 kb on - strand, within RALBFv3_RS23860at 2073.346 kb on - strand, within RALBFv3_RS23860at 2073.346 kb on - strand, within RALBFv3_RS23860at 2073.346 kb on - strand, within RALBFv3_RS23860at 2073.346 kb on - strand, within RALBFv3_RS23860at 2073.357 kb on + strand, within RALBFv3_RS23860at 2073.358 kb on - strand, within RALBFv3_RS23860at 2073.404 kb on + strand, within RALBFv3_RS23860at 2073.404 kb on + strand, within RALBFv3_RS23860at 2073.445 kb on + strand, within RALBFv3_RS23860at 2073.446 kb on - strand, within RALBFv3_RS23860at 2073.446 kb on - strand, within RALBFv3_RS23860at 2073.451 kb on - strand, within RALBFv3_RS23860at 2073.480 kb on + strand, within RALBFv3_RS23860at 2073.544 kb on + strand, within RALBFv3_RS23860at 2073.585 kb on + strand, within RALBFv3_RS23860at 2073.585 kb on + strand, within RALBFv3_RS23860at 2073.586 kb on - strand, within RALBFv3_RS23860at 2073.586 kb on - strand, within RALBFv3_RS23860at 2073.586 kb on - strand, within RALBFv3_RS23860at 2073.610 kb on + strand, within RALBFv3_RS23860at 2073.611 kb on - strand, within RALBFv3_RS23860at 2073.611 kb on - strand, within RALBFv3_RS23860at 2073.611 kb on - strand, within RALBFv3_RS23860at 2073.611 kb on - strand, within RALBFv3_RS23860at 2073.611 kb on - strand, within RALBFv3_RS23860at 2073.718 kb on - strand, within RALBFv3_RS23860at 2073.718 kb on - strand, within RALBFv3_RS23860at 2073.718 kb on - strand, within RALBFv3_RS23860at 2073.727 kb on - strand, within RALBFv3_RS23860at 2073.727 kb on - strand, within RALBFv3_RS23860at 2073.757 kb on + strand, within RALBFv3_RS23860at 2073.757 kb on + strand, within RALBFv3_RS23860at 2073.758 kb on - strand, within RALBFv3_RS23860at 2073.797 kb on + strand, within RALBFv3_RS23860at 2073.797 kb on + strand, within RALBFv3_RS23860at 2073.797 kb on + strand, within RALBFv3_RS23860at 2073.797 kb on + strand, within RALBFv3_RS23860at 2073.798 kb on - strand, within RALBFv3_RS23860at 2073.798 kb on - strand, within RALBFv3_RS23860at 2073.905 kb on - strand, within RALBFv3_RS23860at 2073.909 kb on + strand, within RALBFv3_RS23860at 2073.909 kb on + strand, within RALBFv3_RS23860at 2073.909 kb on + strand, within RALBFv3_RS23860at 2073.909 kb on + strand, within RALBFv3_RS23860at 2073.910 kb on - strand, within RALBFv3_RS23860at 2073.910 kb on - strand, within RALBFv3_RS23860at 2073.911 kb on - strand, within RALBFv3_RS23860at 2074.004 kb on + strand, within RALBFv3_RS23860at 2074.004 kb on + strand, within RALBFv3_RS23860at 2074.004 kb on + strand, within RALBFv3_RS23860at 2074.005 kb on - strand, within RALBFv3_RS23860at 2074.005 kb on - strand, within RALBFv3_RS23860at 2074.009 kb on + strand, within RALBFv3_RS23860at 2074.009 kb on + strand, within RALBFv3_RS23860at 2074.009 kb on + strand, within RALBFv3_RS23860at 2074.010 kb on - strand, within RALBFv3_RS23860at 2074.012 kb on + strand, within RALBFv3_RS23860at 2074.013 kb on - strand, within RALBFv3_RS23860at 2074.106 kb on - strandat 2074.167 kb on - strandat 2074.205 kb on + strand, within RALBFv3_RS09350at 2074.205 kb on + strand, within RALBFv3_RS09350at 2074.206 kb on - strand, within RALBFv3_RS09350at 2074.206 kb on - strand, within RALBFv3_RS09350at 2074.238 kb on + strand, within RALBFv3_RS09350at 2074.238 kb on + strand, within RALBFv3_RS09350at 2074.246 kb on + strand, within RALBFv3_RS09350at 2074.262 kb on - strand, within RALBFv3_RS09350at 2074.267 kb on - strand, within RALBFv3_RS09350at 2074.291 kb on - strand, within RALBFv3_RS09350at 2074.291 kb on - strand, within RALBFv3_RS09350at 2074.291 kb on - strand, within RALBFv3_RS09350at 2074.298 kb on - strand, within RALBFv3_RS09350at 2074.363 kb on + strand, within RALBFv3_RS09350at 2074.366 kb on + strand, within RALBFv3_RS09350at 2074.402 kb on + strand, within RALBFv3_RS09350at 2074.500 kb on - strand, within RALBFv3_RS09350at 2074.557 kb on + strand, within RALBFv3_RS09350at 2074.557 kb on + strand, within RALBFv3_RS09350at 2074.646 kb on + strand, within RALBFv3_RS09350at 2074.646 kb on + strand, within RALBFv3_RS09350at 2074.647 kb on - strand, within RALBFv3_RS09350at 2074.706 kb on + strand, within RALBFv3_RS09350at 2074.706 kb on + strand, within RALBFv3_RS09350at 2074.706 kb on + strand, within RALBFv3_RS09350at 2074.707 kb on - strand, within RALBFv3_RS09350at 2074.709 kb on + strand, within RALBFv3_RS09350at 2074.731 kb on - strand, within RALBFv3_RS09350at 2074.731 kb on - strand, within RALBFv3_RS09350at 2074.755 kb on - strand, within RALBFv3_RS09350at 2074.769 kb on + strand, within RALBFv3_RS09350at 2074.769 kb on + strand, within RALBFv3_RS09350at 2074.769 kb on + strand, within RALBFv3_RS09350at 2074.770 kb on - strand, within RALBFv3_RS09350at 2074.770 kb on - strand, within RALBFv3_RS09350at 2074.859 kb on + strand, within RALBFv3_RS09350at 2074.860 kb on - strand, within RALBFv3_RS09350at 2074.899 kb on + strand, within RALBFv3_RS09350at 2074.918 kb on + strand, within RALBFv3_RS09350at 2074.982 kb on + strand, within RALBFv3_RS09350at 2074.982 kb on + strand, within RALBFv3_RS09350at 2074.996 kb on + strandat 2074.997 kb on - strandat 2075.000 kb on + strandat 2075.014 kb on + strandat 2075.014 kb on + strandat 2075.015 kb on - strandat 2075.023 kb on + strandat 2075.025 kb on - strandat 2075.050 kb on + strandat 2075.095 kb on + strandat 2075.096 kb on - strandat 2075.096 kb on - strandat 2075.096 kb on - strandat 2075.096 kb on - strandat 2075.096 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction Plant=Tomato_cv_Moneymaker; PlantTreatment=None; Sample=Xylem_sap; GrowthSubstrate=calcine_clay; Collection=direct; 1 dpi
remove
2,072,067 + RALBFv3_RS23855 0.35 -0.6
2,072,067 + RALBFv3_RS23855 0.35 +0.2
2,072,067 + RALBFv3_RS23855 0.35 -0.3
2,072,067 + RALBFv3_RS23855 0.35 +2.0
2,072,068 - RALBFv3_RS23855 0.35 -0.4
2,072,068 - RALBFv3_RS23855 0.35 +1.0
2,072,068 - RALBFv3_RS23855 0.35 +0.2
2,072,068 - RALBFv3_RS23855 0.35 +2.0
2,072,068 - RALBFv3_RS23855 0.35 -0.3
2,072,068 - RALBFv3_RS23855 0.35 -0.2
2,072,068 - RALBFv3_RS23855 0.35 +2.8
2,072,070 + RALBFv3_RS23855 0.36 -0.6
2,072,070 + RALBFv3_RS23855 0.36 -0.6
2,072,129 - RALBFv3_RS23855 0.40 -1.8
2,072,133 + RALBFv3_RS23855 0.40 +0.1
2,072,149 - RALBFv3_RS23855 0.41 +0.3
2,072,331 + RALBFv3_RS23855 0.55 +0.1
2,072,332 - RALBFv3_RS23855 0.55 +0.4
2,072,350 + RALBFv3_RS23855 0.56 +0.3
2,072,441 + RALBFv3_RS23855 0.63 +0.5
2,072,441 + RALBFv3_RS23855 0.63 -0.8
2,072,442 - RALBFv3_RS23855 0.63 +0.2
2,072,451 + RALBFv3_RS23855 0.63 -0.6
2,072,452 - RALBFv3_RS23855 0.64 -0.1
2,072,499 - RALBFv3_RS23855 0.67 +0.5
2,072,499 - RALBFv3_RS23855 0.67 -0.3
2,072,499 - RALBFv3_RS23855 0.67 -0.2
2,072,530 + RALBFv3_RS23855 0.69 +0.1
2,072,530 + RALBFv3_RS23855 0.69 +0.2
2,072,530 + RALBFv3_RS23855 0.69 -0.3
2,072,530 + RALBFv3_RS23855 0.69 +0.5
2,072,531 - RALBFv3_RS23855 0.69 +0.3
2,072,543 + RALBFv3_RS23855 0.70 +0.9
2,072,544 - RALBFv3_RS23855 0.70 +0.3
2,072,544 - RALBFv3_RS23855 0.70 +0.3
2,072,544 - RALBFv3_RS23855 0.70 -0.9
2,072,668 - RALBFv3_RS23855 0.79 -0.6
2,072,668 - RALBFv3_RS23855 0.79 -0.2
2,072,668 - RALBFv3_RS23855 0.79 -1.7
2,072,669 - RALBFv3_RS23855 0.79 -0.9
2,072,691 + RALBFv3_RS23855 0.81 -0.6
2,072,692 - RALBFv3_RS23855 0.81 -0.1
2,072,722 + RALBFv3_RS23855 0.83 +2.0
2,072,744 + RALBFv3_RS23855 0.85 -0.4
2,072,744 + RALBFv3_RS23855 0.85 -0.0
2,072,744 + RALBFv3_RS23855 0.85 -0.0
2,072,745 - RALBFv3_RS23855 0.85 +0.1
2,072,745 - RALBFv3_RS23855 0.85 +0.1
2,072,765 + RALBFv3_RS23855 0.86 -0.5
2,072,765 + RALBFv3_RS23855 0.86 +1.0
2,072,765 + RALBFv3_RS23855 0.86 +0.3
2,072,766 - RALBFv3_RS23855 0.86 +0.4
2,072,766 - RALBFv3_RS23855 0.86 +0.0
2,072,766 - RALBFv3_RS23855 0.86 +0.9
2,072,766 - RALBFv3_RS23855 0.86 -0.5
2,072,766 - RALBFv3_RS23855 0.86 -0.8
2,072,766 - RALBFv3_RS23855 0.86 +0.2
2,072,770 + RALBFv3_RS23855 0.87 +0.0
2,072,771 - RALBFv3_RS23855 0.87 +0.4
2,072,876 + +0.8
2,072,876 + +1.8
2,072,898 + -0.5
2,072,898 + +1.0
2,072,899 - -0.3
2,072,942 + +0.6
2,072,973 - +0.3
2,072,973 - +0.4
2,073,047 - +0.4
2,073,081 + +0.0
2,073,082 - -0.1
2,073,125 + RALBFv3_RS23860 0.11 +0.4
2,073,155 - RALBFv3_RS23860 0.13 -0.7
2,073,214 - RALBFv3_RS23860 0.19 -0.6
2,073,282 - RALBFv3_RS23860 0.25 +0.3
2,073,301 + RALBFv3_RS23860 0.26 +0.4
2,073,302 - RALBFv3_RS23860 0.26 -0.1
2,073,346 - RALBFv3_RS23860 0.30 -0.9
2,073,346 - RALBFv3_RS23860 0.30 -0.1
2,073,346 - RALBFv3_RS23860 0.30 +1.1
2,073,346 - RALBFv3_RS23860 0.30 +1.3
2,073,357 + RALBFv3_RS23860 0.31 -0.1
2,073,358 - RALBFv3_RS23860 0.31 +0.3
2,073,404 + RALBFv3_RS23860 0.35 -0.1
2,073,404 + RALBFv3_RS23860 0.35 -0.1
2,073,445 + RALBFv3_RS23860 0.39 -1.1
2,073,446 - RALBFv3_RS23860 0.39 +0.6
2,073,446 - RALBFv3_RS23860 0.39 -0.3
2,073,451 - RALBFv3_RS23860 0.40 +0.3
2,073,480 + RALBFv3_RS23860 0.42 +0.2
2,073,544 + RALBFv3_RS23860 0.48 -0.3
2,073,585 + RALBFv3_RS23860 0.52 -0.4
2,073,585 + RALBFv3_RS23860 0.52 +0.7
2,073,586 - RALBFv3_RS23860 0.52 +0.2
2,073,586 - RALBFv3_RS23860 0.52 -0.2
2,073,586 - RALBFv3_RS23860 0.52 +1.0
2,073,610 + RALBFv3_RS23860 0.54 +0.4
2,073,611 - RALBFv3_RS23860 0.54 +0.2
2,073,611 - RALBFv3_RS23860 0.54 +3.0
2,073,611 - RALBFv3_RS23860 0.54 +0.1
2,073,611 - RALBFv3_RS23860 0.54 +0.4
2,073,611 - RALBFv3_RS23860 0.54 +0.2
2,073,718 - RALBFv3_RS23860 0.63 +3.0
2,073,718 - RALBFv3_RS23860 0.63 +0.0
2,073,718 - RALBFv3_RS23860 0.63 +0.3
2,073,727 - RALBFv3_RS23860 0.64 +0.2
2,073,727 - RALBFv3_RS23860 0.64 -0.3
2,073,757 + RALBFv3_RS23860 0.67 -0.3
2,073,757 + RALBFv3_RS23860 0.67 -0.4
2,073,758 - RALBFv3_RS23860 0.67 -0.5
2,073,797 + RALBFv3_RS23860 0.70 -0.3
2,073,797 + RALBFv3_RS23860 0.70 -0.5
2,073,797 + RALBFv3_RS23860 0.70 +0.5
2,073,797 + RALBFv3_RS23860 0.70 -0.0
2,073,798 - RALBFv3_RS23860 0.70 +0.3
2,073,798 - RALBFv3_RS23860 0.70 +1.2
2,073,905 - RALBFv3_RS23860 0.80 -0.4
2,073,909 + RALBFv3_RS23860 0.80 +1.0
2,073,909 + RALBFv3_RS23860 0.80 +0.4
2,073,909 + RALBFv3_RS23860 0.80 +1.4
2,073,909 + RALBFv3_RS23860 0.80 +0.4
2,073,910 - RALBFv3_RS23860 0.80 +0.2
2,073,910 - RALBFv3_RS23860 0.80 -0.3
2,073,911 - RALBFv3_RS23860 0.80 +0.1
2,074,004 + RALBFv3_RS23860 0.89 -0.5
2,074,004 + RALBFv3_RS23860 0.89 -0.3
2,074,004 + RALBFv3_RS23860 0.89 -0.3
2,074,005 - RALBFv3_RS23860 0.89 -0.6
2,074,005 - RALBFv3_RS23860 0.89 -0.1
2,074,009 + RALBFv3_RS23860 0.89 +0.1
2,074,009 + RALBFv3_RS23860 0.89 -0.6
2,074,009 + RALBFv3_RS23860 0.89 -0.4
2,074,010 - RALBFv3_RS23860 0.89 +0.3
2,074,012 + RALBFv3_RS23860 0.89 -0.1
2,074,013 - RALBFv3_RS23860 0.89 +0.6
2,074,106 - -0.4
2,074,167 - +0.2
2,074,205 + RALBFv3_RS09350 0.13 -0.6
2,074,205 + RALBFv3_RS09350 0.13 +0.7
2,074,206 - RALBFv3_RS09350 0.13 +0.4
2,074,206 - RALBFv3_RS09350 0.13 +0.1
2,074,238 + RALBFv3_RS09350 0.16 -0.6
2,074,238 + RALBFv3_RS09350 0.16 -0.1
2,074,246 + RALBFv3_RS09350 0.17 -1.2
2,074,262 - RALBFv3_RS09350 0.19 +0.0
2,074,267 - RALBFv3_RS09350 0.19 -0.6
2,074,291 - RALBFv3_RS09350 0.22 -0.1
2,074,291 - RALBFv3_RS09350 0.22 -1.2
2,074,291 - RALBFv3_RS09350 0.22 -0.1
2,074,298 - RALBFv3_RS09350 0.22 -0.1
2,074,363 + RALBFv3_RS09350 0.29 -0.1
2,074,366 + RALBFv3_RS09350 0.29 +0.2
2,074,402 + RALBFv3_RS09350 0.32 +0.3
2,074,500 - RALBFv3_RS09350 0.42 +0.1
2,074,557 + RALBFv3_RS09350 0.47 -0.3
2,074,557 + RALBFv3_RS09350 0.47 +0.9
2,074,646 + RALBFv3_RS09350 0.56 +0.1
2,074,646 + RALBFv3_RS09350 0.56 +0.3
2,074,647 - RALBFv3_RS09350 0.56 -0.0
2,074,706 + RALBFv3_RS09350 0.62 +0.1
2,074,706 + RALBFv3_RS09350 0.62 -0.1
2,074,706 + RALBFv3_RS09350 0.62 +0.8
2,074,707 - RALBFv3_RS09350 0.62 -0.1
2,074,709 + RALBFv3_RS09350 0.62 -0.1
2,074,731 - RALBFv3_RS09350 0.64 +0.7
2,074,731 - RALBFv3_RS09350 0.64 -0.0
2,074,755 - RALBFv3_RS09350 0.67 -0.1
2,074,769 + RALBFv3_RS09350 0.68 +1.6
2,074,769 + RALBFv3_RS09350 0.68 -1.2
2,074,769 + RALBFv3_RS09350 0.68 +0.2
2,074,770 - RALBFv3_RS09350 0.68 -0.2
2,074,770 - RALBFv3_RS09350 0.68 +0.2
2,074,859 + RALBFv3_RS09350 0.77 -0.2
2,074,860 - RALBFv3_RS09350 0.77 +0.2
2,074,899 + RALBFv3_RS09350 0.81 +0.0
2,074,918 + RALBFv3_RS09350 0.83 +0.0
2,074,982 + RALBFv3_RS09350 0.89 +0.5
2,074,982 + RALBFv3_RS09350 0.89 -0.2
2,074,996 + +0.9
2,074,997 - -0.1
2,075,000 + +0.2
2,075,014 + +1.0
2,075,014 + -0.0
2,075,015 - +0.0
2,075,023 + +0.3
2,075,025 - -1.0
2,075,050 + +0.2
2,075,095 + +0.2
2,075,096 - +1.1
2,075,096 - -0.4
2,075,096 - +0.7
2,075,096 - +0.7
2,075,096 - +0.8

Or see this region's nucleotide sequence