Experiment: Growth at pH9 and (C) D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS26575 and PFLU_RS26580 are separated by 123 nucleotides PFLU_RS26580 and PFLU_RS26585 are separated by 98 nucleotides PFLU_RS26585 and PFLU_RS26590 are separated by 262 nucleotides
PFLU_RS26575: PFLU_RS26575 - apolipoprotein N-acyltransferase, at 5,939,950 to 5,941,473
_RS26575
PFLU_RS26580: PFLU_RS26580 - YdcF family protein, at 5,941,597 to 5,942,358
_RS26580
PFLU_RS26585: PFLU_RS26585 - hypothetical protein, at 5,942,457 to 5,942,774
_RS26585
PFLU_RS26590: PFLU_RS26590 - leucine--tRNA ligase, at 5,943,037 to 5,945,643
_RS26590
Position (kb) 5941
5942
5943 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 5941.486 kb on + strand at 5941.486 kb on + strand at 5941.486 kb on + strand at 5941.486 kb on + strand at 5941.487 kb on - strand at 5941.487 kb on - strand at 5941.487 kb on - strand at 5941.487 kb on - strand at 5941.487 kb on - strand at 5941.490 kb on + strand at 5941.490 kb on + strand at 5941.490 kb on + strand at 5941.491 kb on - strand at 5941.491 kb on - strand at 5941.491 kb on - strand at 5941.491 kb on - strand at 5941.491 kb on - strand at 5941.491 kb on - strand at 5941.491 kb on - strand at 5941.491 kb on - strand at 5941.491 kb on - strand at 5941.491 kb on - strand at 5941.491 kb on - strand at 5941.491 kb on - strand at 5941.491 kb on - strand at 5941.491 kb on - strand at 5941.491 kb on - strand at 5941.543 kb on + strand at 5941.543 kb on + strand at 5941.543 kb on + strand at 5941.549 kb on + strand at 5941.550 kb on - strand at 5941.550 kb on - strand at 5941.550 kb on - strand at 5941.550 kb on - strand at 5941.550 kb on - strand at 5941.550 kb on - strand at 5941.550 kb on - strand at 5941.550 kb on - strand at 5941.550 kb on - strand at 5941.550 kb on - strand at 5941.605 kb on - strand at 5941.621 kb on + strand at 5941.621 kb on + strand at 5941.621 kb on + strand at 5941.621 kb on + strand at 5941.621 kb on + strand at 5941.621 kb on + strand at 5941.622 kb on - strand at 5941.622 kb on - strand at 5941.622 kb on - strand at 5941.622 kb on - strand at 5941.622 kb on - strand at 5941.747 kb on - strand, within PFLU_RS26580 at 5941.785 kb on + strand, within PFLU_RS26580 at 5941.786 kb on - strand, within PFLU_RS26580 at 5941.786 kb on - strand, within PFLU_RS26580 at 5941.786 kb on - strand, within PFLU_RS26580 at 5941.786 kb on - strand, within PFLU_RS26580 at 5941.786 kb on - strand, within PFLU_RS26580 at 5941.821 kb on + strand, within PFLU_RS26580 at 5941.873 kb on - strand, within PFLU_RS26580 at 5941.873 kb on - strand, within PFLU_RS26580 at 5941.873 kb on - strand, within PFLU_RS26580 at 5941.873 kb on - strand, within PFLU_RS26580 at 5941.964 kb on + strand, within PFLU_RS26580 at 5942.024 kb on + strand, within PFLU_RS26580 at 5942.025 kb on - strand, within PFLU_RS26580 at 5942.272 kb on - strand, within PFLU_RS26580 at 5942.345 kb on + strand at 5942.345 kb on + strand at 5942.345 kb on + strand at 5942.345 kb on + strand at 5942.345 kb on + strand at 5942.346 kb on - strand at 5942.346 kb on - strand at 5942.352 kb on + strand at 5942.353 kb on - strand at 5942.371 kb on + strand at 5942.371 kb on + strand at 5942.371 kb on + strand at 5942.371 kb on + strand at 5942.371 kb on + strand at 5942.371 kb on + strand at 5942.371 kb on + strand at 5942.371 kb on + strand at 5942.371 kb on + strand at 5942.371 kb on + strand at 5942.371 kb on + strand at 5942.371 kb on + strand at 5942.371 kb on + strand at 5942.371 kb on + strand at 5942.371 kb on + strand at 5942.371 kb on + strand at 5942.371 kb on + strand at 5942.371 kb on + strand at 5942.371 kb on + strand at 5942.371 kb on + strand at 5942.371 kb on + strand at 5942.371 kb on + strand at 5942.371 kb on + strand at 5942.372 kb on - strand at 5942.372 kb on - strand at 5942.372 kb on - strand at 5942.372 kb on - strand at 5942.372 kb on - strand at 5942.372 kb on - strand at 5942.372 kb on - strand at 5942.372 kb on - strand at 5942.372 kb on - strand at 5942.372 kb on - strand at 5942.372 kb on - strand at 5942.372 kb on - strand at 5942.372 kb on - strand at 5942.372 kb on - strand at 5942.372 kb on - strand at 5942.372 kb on - strand at 5942.372 kb on - strand at 5942.372 kb on - strand at 5942.372 kb on - strand at 5942.386 kb on + strand at 5942.386 kb on + strand at 5942.387 kb on - strand at 5942.387 kb on - strand at 5942.387 kb on - strand at 5942.393 kb on + strand at 5942.393 kb on + strand at 5942.393 kb on + strand at 5942.394 kb on - strand at 5942.403 kb on + strand at 5942.404 kb on - strand at 5942.416 kb on + strand at 5942.416 kb on + strand at 5942.417 kb on - strand at 5942.458 kb on + strand at 5942.458 kb on + strand at 5942.458 kb on + strand at 5942.458 kb on + strand at 5942.458 kb on + strand at 5942.458 kb on + strand at 5942.458 kb on + strand at 5942.458 kb on + strand at 5942.458 kb on + strand at 5942.458 kb on + strand at 5942.459 kb on - strand at 5942.459 kb on - strand at 5942.459 kb on - strand at 5942.459 kb on - strand at 5942.459 kb on - strand at 5942.465 kb on - strand at 5942.540 kb on + strand, within PFLU_RS26585 at 5942.540 kb on + strand, within PFLU_RS26585 at 5942.540 kb on + strand, within PFLU_RS26585 at 5942.541 kb on - strand, within PFLU_RS26585 at 5942.541 kb on - strand, within PFLU_RS26585 at 5942.541 kb on - strand, within PFLU_RS26585 at 5942.541 kb on - strand, within PFLU_RS26585 at 5942.591 kb on + strand, within PFLU_RS26585 at 5942.591 kb on + strand, within PFLU_RS26585 at 5942.591 kb on + strand, within PFLU_RS26585 at 5942.591 kb on + strand, within PFLU_RS26585 at 5942.592 kb on - strand, within PFLU_RS26585 at 5942.592 kb on - strand, within PFLU_RS26585 at 5942.592 kb on - strand, within PFLU_RS26585 at 5942.592 kb on - strand, within PFLU_RS26585 at 5942.592 kb on - strand, within PFLU_RS26585 at 5942.592 kb on - strand, within PFLU_RS26585 at 5942.592 kb on - strand, within PFLU_RS26585 at 5942.592 kb on - strand, within PFLU_RS26585 at 5942.592 kb on - strand, within PFLU_RS26585 at 5942.592 kb on - strand, within PFLU_RS26585 at 5942.602 kb on + strand, within PFLU_RS26585 at 5942.602 kb on + strand, within PFLU_RS26585 at 5942.603 kb on - strand, within PFLU_RS26585 at 5942.603 kb on - strand, within PFLU_RS26585 at 5942.603 kb on - strand, within PFLU_RS26585 at 5942.603 kb on - strand, within PFLU_RS26585 at 5942.734 kb on + strand, within PFLU_RS26585 at 5942.734 kb on + strand, within PFLU_RS26585 at 5942.734 kb on + strand, within PFLU_RS26585 at 5942.734 kb on + strand, within PFLU_RS26585 at 5942.734 kb on + strand, within PFLU_RS26585 at 5942.734 kb on + strand, within PFLU_RS26585 at 5942.734 kb on + strand, within PFLU_RS26585 at 5942.734 kb on + strand, within PFLU_RS26585 at 5942.734 kb on + strand, within PFLU_RS26585 at 5942.734 kb on + strand, within PFLU_RS26585 at 5942.734 kb on + strand, within PFLU_RS26585 at 5942.734 kb on + strand, within PFLU_RS26585 at 5942.734 kb on + strand, within PFLU_RS26585 at 5942.735 kb on - strand, within PFLU_RS26585 at 5942.735 kb on - strand, within PFLU_RS26585 at 5942.735 kb on - strand, within PFLU_RS26585 at 5942.735 kb on - strand, within PFLU_RS26585 at 5942.735 kb on - strand, within PFLU_RS26585 at 5942.735 kb on - strand, within PFLU_RS26585 at 5942.735 kb on - strand, within PFLU_RS26585 at 5942.735 kb on - strand, within PFLU_RS26585 at 5942.735 kb on - strand, within PFLU_RS26585 at 5942.735 kb on - strand, within PFLU_RS26585 at 5942.735 kb on - strand, within PFLU_RS26585 at 5942.735 kb on - strand, within PFLU_RS26585 at 5942.735 kb on - strand, within PFLU_RS26585 at 5942.735 kb on - strand, within PFLU_RS26585 at 5942.735 kb on - strand, within PFLU_RS26585 at 5942.735 kb on - strand, within PFLU_RS26585 at 5942.735 kb on - strand, within PFLU_RS26585 at 5942.739 kb on - strand, within PFLU_RS26585 at 5942.794 kb on + strand at 5942.794 kb on + strand at 5942.794 kb on + strand at 5942.794 kb on + strand at 5942.794 kb on + strand at 5942.794 kb on + strand at 5942.794 kb on + strand at 5942.794 kb on + strand at 5942.794 kb on + strand at 5942.795 kb on - strand at 5942.795 kb on - strand at 5942.795 kb on - strand at 5942.795 kb on - strand at 5942.811 kb on + strand at 5942.812 kb on - strand at 5942.812 kb on - strand at 5942.820 kb on + strand at 5942.835 kb on + strand at 5942.835 kb on + strand at 5942.835 kb on + strand at 5942.835 kb on + strand at 5942.835 kb on + strand at 5942.835 kb on + strand at 5942.836 kb on - strand at 5942.836 kb on - strand at 5942.836 kb on - strand at 5942.891 kb on + strand at 5942.891 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.906 kb on + strand at 5942.907 kb on - strand at 5942.907 kb on - strand at 5942.907 kb on - strand at 5942.907 kb on - strand at 5942.907 kb on - strand at 5942.907 kb on - strand at 5942.925 kb on + strand at 5942.925 kb on + strand at 5942.925 kb on + strand at 5942.925 kb on + strand at 5942.925 kb on + strand at 5942.925 kb on + strand at 5943.006 kb on + strand at 5943.017 kb on + strand at 5943.017 kb on + strand at 5943.017 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at pH9 and (C) D-Glucose remove 5,941,486 + -0.1 5,941,486 + -2.1 5,941,486 + -1.5 5,941,486 + +0.9 5,941,487 - +1.3 5,941,487 - +3.2 5,941,487 - -1.2 5,941,487 - -3.1 5,941,487 - +1.6 5,941,490 + -1.9 5,941,490 + +1.6 5,941,490 + -1.2 5,941,491 - -0.9 5,941,491 - -1.3 5,941,491 - +0.8 5,941,491 - +1.3 5,941,491 - +0.8 5,941,491 - -1.4 5,941,491 - -0.1 5,941,491 - +1.1 5,941,491 - -0.3 5,941,491 - -0.7 5,941,491 - +0.5 5,941,491 - +0.7 5,941,491 - -0.5 5,941,491 - -1.4 5,941,491 - +2.2 5,941,543 + -0.9 5,941,543 + +0.7 5,941,543 + -1.2 5,941,549 + +1.3 5,941,550 - -2.2 5,941,550 - -1.2 5,941,550 - +1.3 5,941,550 - +2.9 5,941,550 - -1.3 5,941,550 - -0.4 5,941,550 - -2.1 5,941,550 - -0.1 5,941,550 - -0.4 5,941,550 - +0.6 5,941,605 - +1.3 5,941,621 + +0.3 5,941,621 + +0.6 5,941,621 + +1.3 5,941,621 + +0.6 5,941,621 + +2.7 5,941,621 + +0.5 5,941,622 - -2.8 5,941,622 - -1.7 5,941,622 - -1.7 5,941,622 - -0.5 5,941,622 - -0.5 5,941,747 - PFLU_RS26580 0.20 -2.1 5,941,785 + PFLU_RS26580 0.25 -0.6 5,941,786 - PFLU_RS26580 0.25 +1.9 5,941,786 - PFLU_RS26580 0.25 -0.2 5,941,786 - PFLU_RS26580 0.25 -0.7 5,941,786 - PFLU_RS26580 0.25 -0.9 5,941,786 - PFLU_RS26580 0.25 -1.4 5,941,821 + PFLU_RS26580 0.29 -0.4 5,941,873 - PFLU_RS26580 0.36 -0.7 5,941,873 - PFLU_RS26580 0.36 +0.1 5,941,873 - PFLU_RS26580 0.36 -0.5 5,941,873 - PFLU_RS26580 0.36 +2.7 5,941,964 + PFLU_RS26580 0.48 -1.9 5,942,024 + PFLU_RS26580 0.56 +0.6 5,942,025 - PFLU_RS26580 0.56 +0.9 5,942,272 - PFLU_RS26580 0.89 -2.5 5,942,345 + +0.3 5,942,345 + +2.3 5,942,345 + -0.8 5,942,345 + +1.7 5,942,345 + -1.5 5,942,346 - +0.0 5,942,346 - -1.2 5,942,352 + +0.9 5,942,353 - +0.7 5,942,371 + +1.3 5,942,371 + +0.3 5,942,371 + -0.5 5,942,371 + -1.4 5,942,371 + -0.4 5,942,371 + -0.7 5,942,371 + +0.6 5,942,371 + +2.1 5,942,371 + +1.5 5,942,371 + -0.5 5,942,371 + -1.0 5,942,371 + +1.9 5,942,371 + -0.1 5,942,371 + -0.3 5,942,371 + -0.5 5,942,371 + -1.6 5,942,371 + +0.7 5,942,371 + +0.7 5,942,371 + -2.4 5,942,371 + +0.3 5,942,371 + -0.2 5,942,371 + -0.7 5,942,371 + -0.8 5,942,372 - -1.8 5,942,372 - +0.7 5,942,372 - +2.4 5,942,372 - +0.6 5,942,372 - +0.9 5,942,372 - -0.5 5,942,372 - +0.3 5,942,372 - -1.1 5,942,372 - -1.7 5,942,372 - -1.1 5,942,372 - -0.2 5,942,372 - +1.7 5,942,372 - +1.3 5,942,372 - +1.6 5,942,372 - -1.1 5,942,372 - -2.8 5,942,372 - -0.5 5,942,372 - +2.3 5,942,372 - +3.0 5,942,386 + +1.3 5,942,386 + -0.2 5,942,387 - -0.7 5,942,387 - +1.1 5,942,387 - +0.5 5,942,393 + +1.5 5,942,393 + -0.5 5,942,393 + +0.3 5,942,394 - +0.9 5,942,403 + +0.3 5,942,404 - -2.2 5,942,416 + +1.5 5,942,416 + +1.0 5,942,417 - -0.4 5,942,458 + -0.5 5,942,458 + -0.1 5,942,458 + -0.8 5,942,458 + +0.6 5,942,458 + -0.1 5,942,458 + +0.2 5,942,458 + -0.6 5,942,458 + +0.5 5,942,458 + +1.4 5,942,458 + +0.6 5,942,459 - +0.5 5,942,459 - -2.4 5,942,459 - -0.2 5,942,459 - -0.7 5,942,459 - -1.4 5,942,465 - +1.4 5,942,540 + PFLU_RS26585 0.26 -2.3 5,942,540 + PFLU_RS26585 0.26 +2.7 5,942,540 + PFLU_RS26585 0.26 -1.3 5,942,541 - PFLU_RS26585 0.26 -1.0 5,942,541 - PFLU_RS26585 0.26 -1.6 5,942,541 - PFLU_RS26585 0.26 +0.2 5,942,541 - PFLU_RS26585 0.26 +0.4 5,942,591 + PFLU_RS26585 0.42 +1.2 5,942,591 + PFLU_RS26585 0.42 +0.4 5,942,591 + PFLU_RS26585 0.42 +1.7 5,942,591 + PFLU_RS26585 0.42 -0.4 5,942,592 - PFLU_RS26585 0.42 +0.5 5,942,592 - PFLU_RS26585 0.42 -2.7 5,942,592 - PFLU_RS26585 0.42 -0.5 5,942,592 - PFLU_RS26585 0.42 -0.5 5,942,592 - PFLU_RS26585 0.42 +0.8 5,942,592 - PFLU_RS26585 0.42 -0.5 5,942,592 - PFLU_RS26585 0.42 -2.4 5,942,592 - PFLU_RS26585 0.42 -2.3 5,942,592 - PFLU_RS26585 0.42 -0.1 5,942,592 - PFLU_RS26585 0.42 -0.8 5,942,602 + PFLU_RS26585 0.46 +0.9 5,942,602 + PFLU_RS26585 0.46 +0.5 5,942,603 - PFLU_RS26585 0.46 -0.1 5,942,603 - PFLU_RS26585 0.46 -0.1 5,942,603 - PFLU_RS26585 0.46 -2.0 5,942,603 - PFLU_RS26585 0.46 +2.4 5,942,734 + PFLU_RS26585 0.87 -0.5 5,942,734 + PFLU_RS26585 0.87 -0.1 5,942,734 + PFLU_RS26585 0.87 +0.5 5,942,734 + PFLU_RS26585 0.87 -2.5 5,942,734 + PFLU_RS26585 0.87 +1.2 5,942,734 + PFLU_RS26585 0.87 -0.6 5,942,734 + PFLU_RS26585 0.87 +1.3 5,942,734 + PFLU_RS26585 0.87 -1.1 5,942,734 + PFLU_RS26585 0.87 -0.3 5,942,734 + PFLU_RS26585 0.87 -0.9 5,942,734 + PFLU_RS26585 0.87 +2.1 5,942,734 + PFLU_RS26585 0.87 +4.4 5,942,734 + PFLU_RS26585 0.87 -0.4 5,942,735 - PFLU_RS26585 0.87 +0.0 5,942,735 - PFLU_RS26585 0.87 -0.6 5,942,735 - PFLU_RS26585 0.87 -0.5 5,942,735 - PFLU_RS26585 0.87 -1.7 5,942,735 - PFLU_RS26585 0.87 -0.1 5,942,735 - PFLU_RS26585 0.87 -0.9 5,942,735 - PFLU_RS26585 0.87 -1.4 5,942,735 - PFLU_RS26585 0.87 -0.1 5,942,735 - PFLU_RS26585 0.87 +0.9 5,942,735 - PFLU_RS26585 0.87 +0.2 5,942,735 - PFLU_RS26585 0.87 -0.4 5,942,735 - PFLU_RS26585 0.87 -1.6 5,942,735 - PFLU_RS26585 0.87 -1.2 5,942,735 - PFLU_RS26585 0.87 +0.4 5,942,735 - PFLU_RS26585 0.87 +0.1 5,942,735 - PFLU_RS26585 0.87 +1.8 5,942,735 - PFLU_RS26585 0.87 +1.2 5,942,739 - PFLU_RS26585 0.89 -2.4 5,942,794 + -0.8 5,942,794 + +1.7 5,942,794 + +0.3 5,942,794 + -1.2 5,942,794 + +0.6 5,942,794 + +1.3 5,942,794 + +2.3 5,942,794 + +1.3 5,942,794 + -1.5 5,942,795 - -0.5 5,942,795 - -0.1 5,942,795 - +0.6 5,942,795 - -1.9 5,942,811 + -1.7 5,942,812 - -1.8 5,942,812 - -1.4 5,942,820 + +0.5 5,942,835 + +0.3 5,942,835 + -2.3 5,942,835 + +3.9 5,942,835 + +0.5 5,942,835 + +2.5 5,942,835 + +0.0 5,942,836 - +0.6 5,942,836 - +1.7 5,942,836 - +0.7 5,942,891 + +1.2 5,942,891 + +2.9 5,942,906 + -0.5 5,942,906 + -2.2 5,942,906 + -0.3 5,942,906 + +1.3 5,942,906 + +1.1 5,942,906 + +0.3 5,942,906 + +2.0 5,942,906 + -0.1 5,942,906 + +0.7 5,942,906 + +0.3 5,942,906 + -0.1 5,942,906 + +0.7 5,942,906 + +2.3 5,942,906 + -1.5 5,942,906 + +0.2 5,942,906 + +1.3 5,942,906 + +0.3 5,942,906 + -0.5 5,942,906 + -0.1 5,942,906 + +2.3 5,942,906 + -1.2 5,942,906 + +1.7 5,942,906 + -0.7 5,942,906 + +0.6 5,942,906 + -0.2 5,942,906 + +0.7 5,942,906 + +0.2 5,942,906 + +2.5 5,942,906 + +1.3 5,942,906 + +2.3 5,942,907 - +3.9 5,942,907 - +2.3 5,942,907 - +2.3 5,942,907 - +1.3 5,942,907 - +0.7 5,942,907 - +2.7 5,942,925 + +1.0 5,942,925 + +2.3 5,942,925 + -0.5 5,942,925 + -1.3 5,942,925 + -0.3 5,942,925 + +0.9 5,943,006 + +2.4 5,943,017 + +1.6 5,943,017 + +0.4 5,943,017 + +0.1
Or see this region's nucleotide sequence