Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS05240

Experiment: Min media with glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS05235 and BPHYT_RS05240 are separated by 2 nucleotidesBPHYT_RS05240 and BPHYT_RS05245 are separated by 151 nucleotides BPHYT_RS05235: BPHYT_RS05235 - bifunctional uroporphyrinogen-III synthetase/uroporphyrin-III C-methyltransferase, at 1,207,588 to 1,209,606 _RS05235 BPHYT_RS05240: BPHYT_RS05240 - heme biosynthesis protein HemY, at 1,209,609 to 1,210,799 _RS05240 BPHYT_RS05245: BPHYT_RS05245 - hypothetical protein, at 1,210,951 to 1,211,418 _RS05245 Position (kb) 1209 1210 1211Strain fitness (log2 ratio) -1 0 1at 1209.628 kb on + strandat 1210.860 kb on + strandat 1210.861 kb on - strandat 1211.087 kb on - strand, within BPHYT_RS05245at 1211.275 kb on + strand, within BPHYT_RS05245at 1211.275 kb on + strand, within BPHYT_RS05245at 1211.510 kb on + strandat 1211.510 kb on + strandat 1211.511 kb on - strandat 1211.665 kb on + strandat 1211.666 kb on - strandat 1211.666 kb on - strandat 1211.705 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Min media with glucose
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1,209,628 + -0.7
1,210,860 + -0.9
1,210,861 - -0.8
1,211,087 - BPHYT_RS05245 0.29 +0.4
1,211,275 + BPHYT_RS05245 0.69 +1.1
1,211,275 + BPHYT_RS05245 0.69 -0.8
1,211,510 + -0.6
1,211,510 + -0.4
1,211,511 - -0.6
1,211,665 + +0.5
1,211,666 - -0.5
1,211,666 - +0.4
1,211,705 + +0.1

Or see this region's nucleotide sequence