Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1937 and Dshi_1938 are separated by 116 nucleotides Dshi_1938 and Dshi_1939 are separated by 116 nucleotides Dshi_1939 and Dshi_1940 are separated by 39 nucleotides
Dshi_1937: Dshi_1937 - protein of unknown function DUF463 YcjX family protein (RefSeq), at 2,025,991 to 2,027,403
_1937
Dshi_1938: Dshi_1938 - tRNA pseudouridine synthase A (RefSeq), at 2,027,520 to 2,028,293
_1938
Dshi_1939: Dshi_1939 - binding-protein-dependent transport systems inner membrane component (RefSeq), at 2,028,410 to 2,029,231
_1939
Dshi_1940: Dshi_1940 - ABC transporter related (RefSeq), at 2,029,271 to 2,030,071
_1940
Position (kb)
2027
2028
2029 Strain fitness (log2 ratio)
-2
-1
0
1 at 2026.617 kb on - strand, within Dshi_1937 at 2026.642 kb on - strand, within Dshi_1937 at 2026.677 kb on + strand, within Dshi_1937 at 2026.757 kb on - strand, within Dshi_1937 at 2027.290 kb on + strand at 2027.298 kb on - strand at 2027.358 kb on - strand at 2027.358 kb on - strand at 2027.358 kb on - strand at 2027.439 kb on - strand at 2027.504 kb on + strand at 2027.515 kb on + strand at 2027.701 kb on - strand, within Dshi_1938 at 2027.752 kb on - strand, within Dshi_1938 at 2027.854 kb on - strand, within Dshi_1938 at 2028.580 kb on - strand, within Dshi_1939 at 2028.596 kb on - strand, within Dshi_1939 at 2028.678 kb on + strand, within Dshi_1939 at 2028.866 kb on - strand, within Dshi_1939 at 2028.966 kb on - strand, within Dshi_1939 at 2028.985 kb on - strand, within Dshi_1939 at 2029.024 kb on + strand, within Dshi_1939 at 2029.107 kb on - strand, within Dshi_1939 at 2029.148 kb on + strand, within Dshi_1939 at 2029.154 kb on - strand at 2029.173 kb on - strand at 2029.212 kb on + strand at 2029.261 kb on + strand at 2029.281 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 2,026,617 - Dshi_1937 0.44 -0.1 2,026,642 - Dshi_1937 0.46 -0.0 2,026,677 + Dshi_1937 0.49 -0.1 2,026,757 - Dshi_1937 0.54 -1.0 2,027,290 + -0.9 2,027,298 - -0.8 2,027,358 - -0.9 2,027,358 - -0.8 2,027,358 - +0.3 2,027,439 - +0.1 2,027,504 + -0.3 2,027,515 + -1.7 2,027,701 - Dshi_1938 0.23 -1.2 2,027,752 - Dshi_1938 0.30 -1.2 2,027,854 - Dshi_1938 0.43 -2.3 2,028,580 - Dshi_1939 0.21 +0.5 2,028,596 - Dshi_1939 0.23 -0.7 2,028,678 + Dshi_1939 0.33 -0.1 2,028,866 - Dshi_1939 0.55 +0.1 2,028,966 - Dshi_1939 0.68 -0.9 2,028,985 - Dshi_1939 0.70 -0.8 2,029,024 + Dshi_1939 0.75 +0.6 2,029,107 - Dshi_1939 0.85 -0.2 2,029,148 + Dshi_1939 0.90 -0.8 2,029,154 - -0.6 2,029,173 - +0.3 2,029,212 + +0.4 2,029,261 + +0.2 2,029,281 + -0.0
Or see this region's nucleotide sequence