Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0847 and Dshi_0848 overlap by 4 nucleotides Dshi_0848 and Dshi_0849 are separated by 125 nucleotides Dshi_0849 and Dshi_0850 are separated by 59 nucleotides
Dshi_0847: Dshi_0847 - Dihydroorotate oxidase (RefSeq), at 856,488 to 857,546
_0847
Dshi_0848: Dshi_0848 - protein of unknown function DUF952 (RefSeq), at 857,543 to 857,878
_0848
Dshi_0849: Dshi_0849 - 5'-Nucleotidase domain protein (RefSeq), at 858,004 to 859,593
_0849
Dshi_0850: Dshi_0850 - DEAD/DEAH box helicase domain protein (RefSeq), at 859,653 to 861,845
_0850
Position (kb) 858
859
860 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 857.103 kb on - strand, within Dshi_0847 at 857.277 kb on + strand, within Dshi_0847 at 857.341 kb on - strand, within Dshi_0847 at 857.388 kb on - strand, within Dshi_0847 at 857.443 kb on - strand at 857.463 kb on - strand at 857.611 kb on - strand, within Dshi_0848 at 857.673 kb on - strand, within Dshi_0848 at 857.934 kb on + strand at 858.046 kb on - strand at 858.051 kb on - strand at 858.194 kb on - strand, within Dshi_0849 at 858.307 kb on - strand, within Dshi_0849 at 858.627 kb on + strand, within Dshi_0849 at 858.748 kb on + strand, within Dshi_0849 at 858.781 kb on - strand, within Dshi_0849 at 858.819 kb on + strand, within Dshi_0849 at 858.819 kb on + strand, within Dshi_0849 at 858.912 kb on - strand, within Dshi_0849 at 858.981 kb on + strand, within Dshi_0849 at 858.987 kb on - strand, within Dshi_0849 at 859.235 kb on + strand, within Dshi_0849 at 859.388 kb on + strand, within Dshi_0849 at 859.467 kb on - strand at 859.550 kb on + strand at 859.554 kb on + strand at 859.558 kb on - strand at 859.597 kb on + strand at 859.651 kb on - strand at 859.959 kb on + strand, within Dshi_0850 at 860.044 kb on - strand, within Dshi_0850 at 860.297 kb on - strand, within Dshi_0850 at 860.297 kb on - strand, within Dshi_0850 at 860.324 kb on + strand, within Dshi_0850 at 860.341 kb on - strand, within Dshi_0850 at 860.349 kb on - strand, within Dshi_0850 at 860.385 kb on - strand, within Dshi_0850 at 860.490 kb on - strand, within Dshi_0850 at 860.492 kb on + strand, within Dshi_0850
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 857,103 - Dshi_0847 0.58 -2.9 857,277 + Dshi_0847 0.75 -3.9 857,341 - Dshi_0847 0.81 -2.8 857,388 - Dshi_0847 0.85 -4.0 857,443 - -3.5 857,463 - -3.6 857,611 - Dshi_0848 0.20 -2.4 857,673 - Dshi_0848 0.39 -2.9 857,934 + +1.5 858,046 - -0.2 858,051 - -0.5 858,194 - Dshi_0849 0.12 -0.4 858,307 - Dshi_0849 0.19 -0.5 858,627 + Dshi_0849 0.39 -1.4 858,748 + Dshi_0849 0.47 -0.0 858,781 - Dshi_0849 0.49 -0.1 858,819 + Dshi_0849 0.51 -0.5 858,819 + Dshi_0849 0.51 -0.5 858,912 - Dshi_0849 0.57 -0.7 858,981 + Dshi_0849 0.61 -0.0 858,987 - Dshi_0849 0.62 -1.8 859,235 + Dshi_0849 0.77 -1.6 859,388 + Dshi_0849 0.87 +0.2 859,467 - +0.2 859,550 + -0.5 859,554 + +0.2 859,558 - +0.0 859,597 + -0.0 859,651 - +0.0 859,959 + Dshi_0850 0.14 -0.0 860,044 - Dshi_0850 0.18 -0.2 860,297 - Dshi_0850 0.29 -0.2 860,297 - Dshi_0850 0.29 +0.3 860,324 + Dshi_0850 0.31 -0.8 860,341 - Dshi_0850 0.31 -0.1 860,349 - Dshi_0850 0.32 -2.2 860,385 - Dshi_0850 0.33 +0.4 860,490 - Dshi_0850 0.38 -0.4 860,492 + Dshi_0850 0.38 +0.1
Or see this region's nucleotide sequence