Experiment: H2 deepwell acetate and vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06505 and MMP_RS06510 are separated by 48 nucleotides MMP_RS06510 and MMP_RS06515 are separated by 96 nucleotides MMP_RS06515 and MMP_RS06520 are separated by 138 nucleotides
MMP_RS06505: MMP_RS06505 - Glu-tRNA(Gln) amidotransferase subunit GatE, at 1,248,402 to 1,250,297
_RS06505
MMP_RS06510: MMP_RS06510 - Glu-tRNA(Gln) amidotransferase subunit GatD, at 1,250,346 to 1,251,602
_RS06510
MMP_RS06515: MMP_RS06515 - UPF0489 family protein, at 1,251,699 to 1,252,409
_RS06515
MMP_RS06520: MMP_RS06520 - DUF1786 domain-containing protein, at 1,252,548 to 1,253,564
_RS06520
Position (kb)
1250
1251
1252 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1249.718 kb on - strand at 1250.832 kb on + strand at 1251.556 kb on - strand at 1251.626 kb on - strand at 1251.661 kb on + strand at 1251.687 kb on + strand at 1251.701 kb on + strand at 1251.989 kb on + strand, within MMP_RS06515 at 1252.104 kb on + strand, within MMP_RS06515 at 1252.277 kb on - strand, within MMP_RS06515 at 1252.460 kb on - strand at 1252.461 kb on + strand at 1252.558 kb on - strand at 1252.559 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 deepwell acetate and vitamin FREE remove 1,249,718 - -3.4 1,250,832 + -2.7 1,251,556 - -1.9 1,251,626 - -2.7 1,251,661 + -2.3 1,251,687 + +0.5 1,251,701 + +1.1 1,251,989 + MMP_RS06515 0.41 +0.2 1,252,104 + MMP_RS06515 0.57 +2.3 1,252,277 - MMP_RS06515 0.81 +0.9 1,252,460 - +0.5 1,252,461 + -0.2 1,252,558 - -2.1 1,252,559 + -0.1
Or see this region's nucleotide sequence