Experiment: R2A with Chloride 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS02440 and LRK54_RS02445 are separated by 145 nucleotides LRK54_RS02445 and LRK54_RS02450 are separated by 120 nucleotides
LRK54_RS02440: LRK54_RS02440 - RNA polymerase-binding protein DksA, at 530,679 to 531,923
_RS02440
LRK54_RS02445: LRK54_RS02445 - membrane protein insertion efficiency factor YidD, at 532,069 to 532,311
_RS02445
LRK54_RS02450: LRK54_RS02450 - GTP cyclohydrolase FolE2, at 532,432 to 533,379
_RS02450
Position (kb) 532
533 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 531.162 kb on - strand, within LRK54_RS02440 at 531.300 kb on + strand, within LRK54_RS02440 at 531.301 kb on - strand, within LRK54_RS02440 at 531.565 kb on - strand, within LRK54_RS02440 at 531.565 kb on - strand, within LRK54_RS02440 at 531.905 kb on + strand at 531.905 kb on + strand at 531.905 kb on + strand at 531.905 kb on + strand at 531.905 kb on + strand at 531.905 kb on + strand at 531.905 kb on + strand at 531.905 kb on + strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.906 kb on - strand at 531.924 kb on - strand at 531.924 kb on - strand at 531.924 kb on - strand at 531.924 kb on - strand at 531.924 kb on - strand at 531.924 kb on - strand at 531.960 kb on + strand at 531.960 kb on + strand at 531.960 kb on + strand at 531.960 kb on + strand at 531.960 kb on + strand at 531.961 kb on - strand at 531.961 kb on - strand at 531.961 kb on - strand at 531.961 kb on - strand at 531.961 kb on - strand at 531.961 kb on - strand at 531.961 kb on - strand at 531.961 kb on - strand at 531.961 kb on - strand at 531.961 kb on - strand at 531.961 kb on - strand at 531.962 kb on + strand at 531.962 kb on + strand at 531.962 kb on + strand at 531.962 kb on + strand at 531.962 kb on + strand at 531.962 kb on + strand at 531.963 kb on - strand at 531.963 kb on - strand at 531.963 kb on - strand at 531.963 kb on - strand at 531.963 kb on - strand at 531.963 kb on - strand at 531.963 kb on - strand at 531.963 kb on - strand at 531.963 kb on - strand at 531.963 kb on - strand at 531.963 kb on - strand at 531.963 kb on - strand at 531.976 kb on + strand at 531.977 kb on - strand at 531.977 kb on - strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.001 kb on + strand at 532.002 kb on - strand at 532.002 kb on - strand at 532.002 kb on - strand at 532.002 kb on - strand at 532.002 kb on - strand at 532.002 kb on - strand at 532.002 kb on - strand at 532.002 kb on - strand at 532.002 kb on - strand at 532.002 kb on - strand at 532.002 kb on - strand at 532.002 kb on - strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.007 kb on + strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.008 kb on - strand at 532.013 kb on + strand at 532.013 kb on + strand at 532.013 kb on + strand at 532.013 kb on + strand at 532.013 kb on + strand at 532.013 kb on + strand at 532.013 kb on + strand at 532.013 kb on + strand at 532.014 kb on - strand at 532.014 kb on - strand at 532.014 kb on - strand at 532.014 kb on - strand at 532.014 kb on - strand at 532.014 kb on - strand at 532.014 kb on - strand at 532.014 kb on - strand at 532.014 kb on - strand at 532.014 kb on - strand at 532.061 kb on + strand at 532.061 kb on + strand at 532.061 kb on + strand at 532.061 kb on + strand at 532.061 kb on + strand at 532.061 kb on + strand at 532.061 kb on + strand at 532.061 kb on + strand at 532.061 kb on + strand at 532.061 kb on + strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.062 kb on - strand at 532.081 kb on + strand at 532.081 kb on + strand at 532.081 kb on + strand at 532.081 kb on + strand at 532.081 kb on + strand at 532.081 kb on + strand at 532.081 kb on + strand at 532.081 kb on + strand at 532.081 kb on + strand at 532.081 kb on + strand at 532.082 kb on - strand at 532.082 kb on - strand at 532.082 kb on - strand at 532.082 kb on - strand at 532.082 kb on - strand at 532.082 kb on - strand at 532.082 kb on - strand at 532.082 kb on - strand at 532.082 kb on - strand at 532.082 kb on - strand at 532.144 kb on + strand, within LRK54_RS02445 at 532.144 kb on + strand, within LRK54_RS02445 at 532.144 kb on + strand, within LRK54_RS02445 at 532.144 kb on + strand, within LRK54_RS02445 at 532.144 kb on + strand, within LRK54_RS02445 at 532.144 kb on + strand, within LRK54_RS02445 at 532.145 kb on - strand, within LRK54_RS02445 at 532.379 kb on + strand at 532.379 kb on + strand at 532.379 kb on + strand at 532.380 kb on - strand at 532.380 kb on - strand at 532.380 kb on - strand at 532.380 kb on - strand at 532.448 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 250 mM remove 531,162 - LRK54_RS02440 0.39 -0.6 531,300 + LRK54_RS02440 0.50 -1.7 531,301 - LRK54_RS02440 0.50 -0.6 531,565 - LRK54_RS02440 0.71 -0.0 531,565 - LRK54_RS02440 0.71 -0.6 531,905 + +0.4 531,905 + +2.0 531,905 + -1.0 531,905 + -0.4 531,905 + -1.6 531,905 + -1.9 531,905 + -1.2 531,905 + -1.6 531,906 - -0.4 531,906 - -1.9 531,906 - +1.0 531,906 - -0.9 531,906 - -0.9 531,906 - -2.0 531,906 - -2.4 531,906 - -4.7 531,906 - -0.7 531,906 - -1.9 531,906 - -2.0 531,906 - -1.7 531,906 - -0.0 531,906 - -4.7 531,906 - +0.4 531,906 - -2.0 531,906 - -2.6 531,924 - -0.6 531,924 - -2.8 531,924 - -3.2 531,924 - +1.0 531,924 - -1.0 531,924 - -2.0 531,960 + -1.2 531,960 + -2.0 531,960 + +0.4 531,960 + -0.0 531,960 + -1.6 531,961 - -2.1 531,961 - -1.7 531,961 - +1.1 531,961 - -0.2 531,961 - +0.4 531,961 - +2.0 531,961 - -0.7 531,961 - -1.0 531,961 - +0.0 531,961 - -0.3 531,961 - -1.3 531,962 + -1.2 531,962 + -0.0 531,962 + -2.0 531,962 + -1.0 531,962 + -1.1 531,962 + -0.9 531,963 - -0.8 531,963 - +0.4 531,963 - -1.6 531,963 - -1.5 531,963 - -1.8 531,963 - -0.5 531,963 - -0.0 531,963 - -2.4 531,963 - -0.6 531,963 - +1.1 531,963 - +0.1 531,963 - -0.0 531,976 + -2.0 531,977 - -0.6 531,977 - +0.0 532,001 + -0.6 532,001 + -1.5 532,001 + -0.2 532,001 + +0.4 532,001 + -3.0 532,001 + -0.0 532,001 + -0.5 532,001 + -1.5 532,001 + +0.7 532,001 + -3.1 532,001 + -1.8 532,001 + -0.9 532,001 + -0.9 532,001 + -0.6 532,001 + -0.6 532,001 + -0.6 532,001 + -0.6 532,001 + -0.9 532,001 + -2.5 532,001 + -2.1 532,002 - -1.5 532,002 - -2.3 532,002 - -1.9 532,002 - -2.2 532,002 - +1.5 532,002 - -0.0 532,002 - -0.2 532,002 - -0.1 532,002 - -0.7 532,002 - -1.4 532,002 - +2.4 532,002 - -0.7 532,007 + -1.7 532,007 + -0.5 532,007 + -0.9 532,007 + +0.4 532,007 + -1.2 532,007 + +0.4 532,007 + -1.9 532,007 + -1.4 532,007 + -0.3 532,007 + -0.6 532,007 + +0.4 532,007 + +0.4 532,007 + +0.1 532,007 + -1.4 532,007 + -0.9 532,007 + -0.9 532,007 + -0.4 532,007 + -0.0 532,007 + +0.4 532,007 + -1.0 532,007 + -0.9 532,007 + +0.3 532,007 + -1.0 532,008 - +0.1 532,008 - -3.0 532,008 - +0.7 532,008 - -0.4 532,008 - +0.2 532,008 - -2.0 532,008 - +0.0 532,008 - +0.7 532,008 - -0.1 532,008 - +0.3 532,008 - +0.5 532,008 - +0.4 532,008 - +0.1 532,008 - -0.0 532,008 - -1.3 532,008 - +1.7 532,008 - -0.6 532,008 - -0.6 532,008 - +2.5 532,008 - +0.0 532,008 - +0.7 532,008 - -0.6 532,008 - -0.4 532,008 - -0.0 532,008 - -0.2 532,013 + +0.5 532,013 + -1.3 532,013 + -1.3 532,013 + -0.7 532,013 + -0.4 532,013 + -2.3 532,013 + -1.5 532,013 + -0.4 532,014 - -1.0 532,014 - +1.2 532,014 - -0.0 532,014 - -0.5 532,014 - +1.4 532,014 - +0.1 532,014 - -1.2 532,014 - -1.1 532,014 - +2.0 532,014 - +0.1 532,061 + -2.5 532,061 + -0.4 532,061 + -0.1 532,061 + +0.0 532,061 + +1.4 532,061 + -2.0 532,061 + -1.0 532,061 + -1.3 532,061 + -0.7 532,061 + -1.0 532,062 - -0.1 532,062 - +1.4 532,062 - -0.4 532,062 - -2.3 532,062 - -1.5 532,062 - -0.0 532,062 - -0.6 532,062 - +1.0 532,062 - -2.6 532,062 - -1.7 532,062 - -1.4 532,062 - -0.0 532,062 - -0.7 532,062 - +0.0 532,081 + +1.0 532,081 + -0.9 532,081 + -4.0 532,081 + +0.4 532,081 + -2.5 532,081 + -1.0 532,081 + -1.9 532,081 + -1.0 532,081 + -0.9 532,081 + -2.3 532,082 - -1.0 532,082 - -1.5 532,082 - +0.4 532,082 - +1.0 532,082 - +2.0 532,082 - +2.0 532,082 - -0.0 532,082 - -1.5 532,082 - -1.2 532,082 - -2.3 532,144 + LRK54_RS02445 0.31 -3.1 532,144 + LRK54_RS02445 0.31 -0.9 532,144 + LRK54_RS02445 0.31 -1.5 532,144 + LRK54_RS02445 0.31 -2.4 532,144 + LRK54_RS02445 0.31 -2.3 532,144 + LRK54_RS02445 0.31 -2.1 532,145 - LRK54_RS02445 0.31 -1.2 532,379 + -0.4 532,379 + +1.4 532,379 + -1.4 532,380 - -1.2 532,380 - -0.2 532,380 - -1.0 532,380 - -0.6 532,448 + -1.0
Or see this region's nucleotide sequence