Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS12530

Experiment: Min media with glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS12525 and BPHYT_RS12530 are separated by 204 nucleotidesBPHYT_RS12530 and BPHYT_RS12535 are separated by 281 nucleotidesBPHYT_RS12535 and BPHYT_RS12540 are separated by 44 nucleotides BPHYT_RS12525: BPHYT_RS12525 - adenylosuccinate synthetase, at 2,859,313 to 2,860,659 _RS12525 BPHYT_RS12530: BPHYT_RS12530 - ATP phosphoribosyltransferase regulatory subunit, at 2,860,864 to 2,862,015 _RS12530 BPHYT_RS12535: BPHYT_RS12535 - membrane protein, at 2,862,297 to 2,862,488 _RS12535 BPHYT_RS12540: BPHYT_RS12540 - membrane protein, at 2,862,533 to 2,863,435 _RS12540 Position (kb) 2860 2861 2862 2863Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 2860.692 kb on - strandat 2860.696 kb on - strandat 2860.740 kb on - strandat 2860.796 kb on + strandat 2861.222 kb on + strand, within BPHYT_RS12530at 2861.688 kb on + strand, within BPHYT_RS12530at 2861.709 kb on - strand, within BPHYT_RS12530at 2861.874 kb on + strand, within BPHYT_RS12530at 2861.875 kb on - strand, within BPHYT_RS12530at 2861.875 kb on - strand, within BPHYT_RS12530at 2861.875 kb on - strand, within BPHYT_RS12530at 2861.875 kb on - strandat 2861.876 kb on + strand, within BPHYT_RS12530at 2862.001 kb on + strandat 2862.001 kb on + strandat 2862.001 kb on + strandat 2862.028 kb on - strandat 2862.382 kb on - strand, within BPHYT_RS12535at 2862.496 kb on - strandat 2862.587 kb on - strandat 2862.676 kb on - strand, within BPHYT_RS12540

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Per-strain Table

Position Strand Gene LocusTag Fraction Min media with glucose
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2,860,692 - -0.1
2,860,696 - -0.5
2,860,740 - +0.4
2,860,796 + -0.6
2,861,222 + BPHYT_RS12530 0.31 -2.7
2,861,688 + BPHYT_RS12530 0.72 -3.7
2,861,709 - BPHYT_RS12530 0.73 -4.3
2,861,874 + BPHYT_RS12530 0.88 -6.5
2,861,875 - BPHYT_RS12530 0.88 -5.2
2,861,875 - BPHYT_RS12530 0.88 -3.7
2,861,875 - BPHYT_RS12530 0.88 -4.0
2,861,875 - -1.4
2,861,876 + BPHYT_RS12530 0.88 -5.3
2,862,001 + -4.4
2,862,001 + -1.1
2,862,001 + -1.7
2,862,028 - +0.0
2,862,382 - BPHYT_RS12535 0.44 -0.2
2,862,496 - -0.1
2,862,587 - +0.3
2,862,676 - BPHYT_RS12540 0.16 -2.4

Or see this region's nucleotide sequence