Experiment: Vanillin 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2858 and Shew_2859 are separated by 20 nucleotides Shew_2859 and Shew_2860 overlap by 25 nucleotides Shew_2860 and Shew_2861 overlap by 4 nucleotides
Shew_2858: Shew_2858 - acetyl-CoA acetyltransferase (RefSeq), at 3,389,550 to 3,390,734
_2858
Shew_2859: Shew_2859 - phosphoglycerate mutase (RefSeq), at 3,390,755 to 3,391,429
_2859
Shew_2860: Shew_2860 - acyl-CoA dehydrogenase domain-containing protein (RefSeq), at 3,391,405 to 3,392,703
_2860
Shew_2861: Shew_2861 - aminoglycoside phosphotransferase (RefSeq), at 3,392,700 to 3,393,737
_2861
Position (kb)
3391
3392
3393 Strain fitness (log2 ratio)
-1
0
1 at 3390.432 kb on + strand, within Shew_2858 at 3391.069 kb on + strand, within Shew_2859 at 3391.668 kb on - strand, within Shew_2860 at 3392.075 kb on - strand, within Shew_2860 at 3392.527 kb on + strand, within Shew_2860 at 3392.818 kb on - strand, within Shew_2861 at 3392.848 kb on + strand, within Shew_2861 at 3392.856 kb on - strand, within Shew_2861 at 3392.961 kb on + strand, within Shew_2861 at 3392.961 kb on + strand, within Shew_2861 at 3393.091 kb on - strand, within Shew_2861 at 3393.139 kb on + strand, within Shew_2861 at 3393.139 kb on + strand, within Shew_2861 at 3393.309 kb on - strand, within Shew_2861 at 3393.369 kb on + strand, within Shew_2861 at 3393.371 kb on + strand, within Shew_2861
Per-strain Table
Position Strand Gene LocusTag Fraction Vanillin 2 mM remove 3,390,432 + Shew_2858 0.74 -0.2 3,391,069 + Shew_2859 0.47 +0.0 3,391,668 - Shew_2860 0.20 +0.3 3,392,075 - Shew_2860 0.52 +1.2 3,392,527 + Shew_2860 0.86 -0.2 3,392,818 - Shew_2861 0.11 -0.6 3,392,848 + Shew_2861 0.14 -0.1 3,392,856 - Shew_2861 0.15 -1.3 3,392,961 + Shew_2861 0.25 -0.2 3,392,961 + Shew_2861 0.25 -0.3 3,393,091 - Shew_2861 0.38 +0.2 3,393,139 + Shew_2861 0.42 +0.1 3,393,139 + Shew_2861 0.42 +0.5 3,393,309 - Shew_2861 0.59 +0.1 3,393,369 + Shew_2861 0.64 -1.0 3,393,371 + Shew_2861 0.65 -0.0
Or see this region's nucleotide sequence