Experiment: L-Malic Acid (C) and N2; anaerobic; high light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2281 and Rru_A2282 are separated by 61 nucleotides Rru_A2282 and Rru_A2283 are separated by 72 nucleotides Rru_A2283 and Rru_A2284 overlap by 1 nucleotides Rru_A2284 and Rru_A2285 overlap by 8 nucleotides
Rru_A2281: Rru_A2281 - NifQ (NCBI), at 2,641,157 to 2,641,825
_A2281
Rru_A2282: Rru_A2282 - 4Fe-4S ferredoxin, iron-sulfur binding (NCBI), at 2,641,887 to 2,642,192
_A2282
Rru_A2283: Rru_A2283 - Protein of unknown function DUF269 (NCBI), at 2,642,265 to 2,642,738
_A2283
Rru_A2284: Rru_A2284 - Dinitrogenase iron-molybdenum cofactor biosynthesis (NCBI), at 2,642,738 to 2,643,226
_A2284
Rru_A2285: Rru_A2285 - Nitrogenase molybdenum-iron cofactor biosynthesis protein (NCBI), at 2,643,219 to 2,644,616
_A2285
Position (kb)
2642
2643
2644 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2642.023 kb on + strand, within Rru_A2282 at 2642.024 kb on - strand, within Rru_A2282 at 2642.140 kb on + strand, within Rru_A2282 at 2642.140 kb on + strand, within Rru_A2282 at 2642.140 kb on + strand, within Rru_A2282 at 2642.141 kb on - strand, within Rru_A2282 at 2642.141 kb on - strand, within Rru_A2282 at 2642.386 kb on + strand, within Rru_A2283 at 2642.386 kb on + strand, within Rru_A2283 at 2642.386 kb on + strand, within Rru_A2283 at 2642.387 kb on - strand, within Rru_A2283 at 2642.387 kb on - strand, within Rru_A2283 at 2642.387 kb on - strand, within Rru_A2283 at 2642.387 kb on - strand, within Rru_A2283 at 2642.512 kb on + strand, within Rru_A2283 at 2642.513 kb on - strand, within Rru_A2283 at 2642.513 kb on - strand, within Rru_A2283 at 2642.537 kb on + strand, within Rru_A2283 at 2642.538 kb on - strand, within Rru_A2283 at 2642.641 kb on + strand, within Rru_A2283 at 2642.641 kb on + strand, within Rru_A2283 at 2642.641 kb on + strand, within Rru_A2283 at 2642.641 kb on + strand, within Rru_A2283 at 2642.642 kb on - strand, within Rru_A2283 at 2642.642 kb on - strand, within Rru_A2283 at 2642.645 kb on + strand, within Rru_A2283 at 2642.645 kb on + strand, within Rru_A2283 at 2642.646 kb on - strand, within Rru_A2283 at 2642.758 kb on + strand at 2642.758 kb on + strand at 2642.758 kb on + strand at 2642.759 kb on - strand at 2643.084 kb on + strand, within Rru_A2284 at 2643.085 kb on - strand, within Rru_A2284 at 2643.105 kb on + strand, within Rru_A2284 at 2643.106 kb on - strand, within Rru_A2284 at 2643.106 kb on - strand, within Rru_A2284 at 2643.106 kb on - strand, within Rru_A2284 at 2643.106 kb on - strand, within Rru_A2284 at 2643.106 kb on - strand, within Rru_A2284 at 2643.194 kb on - strand at 2643.372 kb on + strand, within Rru_A2285
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic Acid (C) and N2; anaerobic; high light remove 2,642,023 + Rru_A2282 0.44 +0.2 2,642,024 - Rru_A2282 0.45 +0.9 2,642,140 + Rru_A2282 0.83 -0.4 2,642,140 + Rru_A2282 0.83 -0.5 2,642,140 + Rru_A2282 0.83 -0.8 2,642,141 - Rru_A2282 0.83 +0.9 2,642,141 - Rru_A2282 0.83 -0.4 2,642,386 + Rru_A2283 0.26 +0.7 2,642,386 + Rru_A2283 0.26 -0.9 2,642,386 + Rru_A2283 0.26 -1.3 2,642,387 - Rru_A2283 0.26 -1.1 2,642,387 - Rru_A2283 0.26 -1.0 2,642,387 - Rru_A2283 0.26 -0.6 2,642,387 - Rru_A2283 0.26 +0.9 2,642,512 + Rru_A2283 0.52 +0.9 2,642,513 - Rru_A2283 0.52 -0.4 2,642,513 - Rru_A2283 0.52 -1.6 2,642,537 + Rru_A2283 0.57 -1.2 2,642,538 - Rru_A2283 0.58 -1.4 2,642,641 + Rru_A2283 0.79 -1.0 2,642,641 + Rru_A2283 0.79 -1.1 2,642,641 + Rru_A2283 0.79 -3.2 2,642,641 + Rru_A2283 0.79 -1.5 2,642,642 - Rru_A2283 0.80 -0.8 2,642,642 - Rru_A2283 0.80 -0.4 2,642,645 + Rru_A2283 0.80 -0.8 2,642,645 + Rru_A2283 0.80 -2.1 2,642,646 - Rru_A2283 0.80 -0.4 2,642,758 + -0.7 2,642,758 + -0.7 2,642,758 + -1.1 2,642,759 - -0.3 2,643,084 + Rru_A2284 0.71 -1.9 2,643,085 - Rru_A2284 0.71 -1.1 2,643,105 + Rru_A2284 0.75 -1.0 2,643,106 - Rru_A2284 0.75 -1.4 2,643,106 - Rru_A2284 0.75 +0.2 2,643,106 - Rru_A2284 0.75 -3.2 2,643,106 - Rru_A2284 0.75 -1.3 2,643,106 - Rru_A2284 0.75 -2.4 2,643,194 - -0.1 2,643,372 + Rru_A2285 0.11 -3.0
Or see this region's nucleotide sequence