Experiment: casaminos
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10630 and BPHYT_RS10635 are separated by 308 nucleotides BPHYT_RS10635 and BPHYT_RS10640 are separated by 123 nucleotides BPHYT_RS10640 and BPHYT_RS10645 are separated by 39 nucleotides
BPHYT_RS10630: BPHYT_RS10630 - oxidoreductase, at 2,422,655 to 2,423,401
_RS10630
BPHYT_RS10635: BPHYT_RS10635 - transcriptional regulator, at 2,423,710 to 2,424,624
_RS10635
BPHYT_RS10640: BPHYT_RS10640 - carbonic anhydrase, at 2,424,748 to 2,425,407
_RS10640
BPHYT_RS10645: BPHYT_RS10645 - cyanate hydratase, at 2,425,447 to 2,425,917
_RS10645
Position (kb)
2423
2424
2425 Strain fitness (log2 ratio)
-1
0
1 at 2422.941 kb on + strand, within BPHYT_RS10630 at 2422.941 kb on + strand, within BPHYT_RS10630 at 2422.942 kb on - strand, within BPHYT_RS10630 at 2423.157 kb on + strand, within BPHYT_RS10630 at 2423.415 kb on - strand at 2423.495 kb on + strand at 2423.496 kb on - strand at 2423.496 kb on - strand at 2423.500 kb on - strand at 2423.536 kb on - strand at 2423.540 kb on - strand at 2423.655 kb on - strand at 2423.667 kb on + strand at 2423.832 kb on + strand, within BPHYT_RS10635 at 2424.435 kb on - strand, within BPHYT_RS10635 at 2425.120 kb on + strand, within BPHYT_RS10640 at 2425.429 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction casaminos remove 2,422,941 + BPHYT_RS10630 0.38 +0.2 2,422,941 + BPHYT_RS10630 0.38 +0.6 2,422,942 - BPHYT_RS10630 0.38 -0.3 2,423,157 + BPHYT_RS10630 0.67 +0.4 2,423,415 - +0.8 2,423,495 + +0.1 2,423,496 - -0.5 2,423,496 - -0.4 2,423,500 - -1.1 2,423,536 - -0.2 2,423,540 - -0.0 2,423,655 - -0.4 2,423,667 + +0.9 2,423,832 + BPHYT_RS10635 0.13 -0.7 2,424,435 - BPHYT_RS10635 0.79 -0.8 2,425,120 + BPHYT_RS10640 0.56 -0.8 2,425,429 - +0.1
Or see this region's nucleotide sequence