Experiment: MoYLS4 with methylglyoxal 0.0156 %
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2219 and DvMF_2220 are separated by 85 nucleotides DvMF_2220 and DvMF_2221 are separated by 178 nucleotides DvMF_2221 and DvMF_2222 overlap by 4 nucleotides
DvMF_2219: DvMF_2219 - nuclease (SNase domain protein) (RefSeq), at 2,779,206 to 2,779,823
_2219
DvMF_2220: DvMF_2220 - lipolytic protein G-D-S-L family (RefSeq), at 2,779,909 to 2,780,553
_2220
DvMF_2221: DvMF_2221 - ABC transporter related (RefSeq), at 2,780,732 to 2,781,490
_2221
DvMF_2222: DvMF_2222 - protein of unknown function DUF214 (RefSeq), at 2,781,487 to 2,784,294
_2222
Position (kb)
2779
2780
2781 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2778.965 kb on - strand at 2779.110 kb on + strand at 2779.202 kb on - strand at 2779.315 kb on + strand, within DvMF_2219 at 2779.687 kb on - strand, within DvMF_2219 at 2780.026 kb on + strand, within DvMF_2220 at 2780.034 kb on - strand, within DvMF_2220 at 2780.282 kb on - strand, within DvMF_2220 at 2780.620 kb on - strand at 2780.803 kb on + strand at 2780.886 kb on + strand, within DvMF_2221 at 2781.040 kb on + strand, within DvMF_2221 at 2781.050 kb on - strand, within DvMF_2221 at 2781.060 kb on + strand, within DvMF_2221
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with methylglyoxal 0.0156 % remove 2,778,965 - +1.3 2,779,110 + -1.4 2,779,202 - -1.1 2,779,315 + DvMF_2219 0.18 -0.5 2,779,687 - DvMF_2219 0.78 -0.7 2,780,026 + DvMF_2220 0.18 +0.1 2,780,034 - DvMF_2220 0.19 -0.1 2,780,282 - DvMF_2220 0.58 +0.9 2,780,620 - -0.6 2,780,803 + -1.2 2,780,886 + DvMF_2221 0.20 -0.1 2,781,040 + DvMF_2221 0.41 -3.2 2,781,050 - DvMF_2221 0.42 -0.5 2,781,060 + DvMF_2221 0.43 -1.2
Or see this region's nucleotide sequence