Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2390 and pflA are separated by 103 nucleotides pflA and Shew_2392 are separated by 6 nucleotides Shew_2392 and Shew_2393 are separated by 195 nucleotides
Shew_2390: Shew_2390 - formate acetyltransferase (RefSeq), at 2,802,496 to 2,804,778
_2390
Shew_2391: pflA - pyruvate formate lyase-activating enzyme 1 (RefSeq), at 2,804,882 to 2,805,622
pflA
Shew_2392: Shew_2392 - hypothetical protein (RefSeq), at 2,805,629 to 2,806,072
_2392
Shew_2393: Shew_2393 - acetate kinase (RefSeq), at 2,806,268 to 2,807,464
_2393
Position (kb)
2804
2805
2806 Strain fitness (log2 ratio)
-1
0
1 at 2803.985 kb on - strand, within Shew_2390 at 2804.127 kb on + strand, within Shew_2390 at 2804.127 kb on + strand, within Shew_2390 at 2804.495 kb on - strand, within Shew_2390 at 2804.495 kb on - strand, within Shew_2390 at 2804.551 kb on - strand at 2804.663 kb on - strand at 2804.720 kb on - strand at 2805.149 kb on + strand, within pflA at 2805.154 kb on + strand, within pflA at 2805.162 kb on - strand, within pflA at 2805.162 kb on - strand, within pflA at 2805.183 kb on + strand, within pflA at 2806.022 kb on - strand, within Shew_2392 at 2806.107 kb on - strand at 2806.197 kb on + strand at 2806.370 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,803,985 - Shew_2390 0.65 +0.0 2,804,127 + Shew_2390 0.71 +0.1 2,804,127 + Shew_2390 0.71 +0.6 2,804,495 - Shew_2390 0.88 +0.0 2,804,495 - Shew_2390 0.88 -0.1 2,804,551 - -0.4 2,804,663 - +0.0 2,804,720 - -0.1 2,805,149 + pflA Shew_2391 0.36 -0.3 2,805,154 + pflA Shew_2391 0.37 -0.3 2,805,162 - pflA Shew_2391 0.38 -0.2 2,805,162 - pflA Shew_2391 0.38 -1.3 2,805,183 + pflA Shew_2391 0.41 +0.2 2,806,022 - Shew_2392 0.89 -0.2 2,806,107 - -0.1 2,806,197 + -0.1 2,806,370 - -1.1
Or see this region's nucleotide sequence